Results 41 to 50 of about 8,966 (157)

Standardization of a multiplex real-time PCR test for the identification of Angiostrongylus cantonensis, A. costaricensis and A. vasorum

Biomédica: revista del Instituto Nacional de Salud, 2018
Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M, Jinney Stefany Arias, Luz Elena Velásquez   +2 more
doaj   +1 more source

Effect of substrate thermal resistance on space-domain microchannel [PDF]

, 2009
In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a micro-fluidic device is presented capable of performing FMCA within a microchannel.
Dalton, Tara, Kinahan, David J., Davies, Mark   +2 more
core   +1 more source

Multiplex Polymerase Chain Reaction/Pooled Antibiotic Susceptibility Testing Was Not Associated with Increased Antibiotic Resistance in Management of Complicated Urinary Tract Infections

, 2023
Howard J Korman,1 Mohit Mathur,2 Natalie Luke,2 Dakun Wang,3 Xihua Zhao,4 Michael Levin,1 David L Wenzler,1 David Baunoch5 1Comprehensive Urology Division, Michigan Healthcare Professionals, Royal Oak, MI, USA; 2Department of Medical Affairs, Pathnostics,
Luke N, Wenzler DL, Wang D, Korman HJ, Mathur M, Levin M, Zhao X, Baunoch D   +7 more
core  

Effectiveness of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis

Journal of Microbiology, Immunology and Infection, 2021
Background: Pertussis, is an infectious respiratory disease caused by Bordetella pertussis. The incidence of pertussis has been increasing in South Korea to due to waning vaccine-induced immunity. Culture has a low sensitivity and a long turnaround time (
Se Chang Oh, Soo Min Park, Jian Hur, Eun Young Choi, Hyun Jung Jin, Yu Kyung Kim, Jong Ho Lee, Ji Young Ahn, Jae Min Lee   +8 more
doaj   +1 more source

A multiplex polymerase chain reaction assay for antibiotic stewardship in suspected pneumonia

Diagnostic Microbiology and Infectious Disease, 2020
Multiplexed molecular rapid diagnostic tests (RDTs) may allow for rapid and accurate diagnosis of the microbial etiology of pneumonia. However, little data are available on multiplexed RDTs in pneumonia and their impact on clinical practice.This retrospective study analyzed 659 hospitalized patients for microbiological diagnosis of suspected pneumonia ...
Pickens, Chiagozie, Wunderink, Richard G., Qi, Chao, Mopuru, Haritha, Donnelly, Helen, Powell, Kimberly, Sims, Matthew D   +6 more
openaire   +2 more sources

Performance comparison of culture against multiplex real-time polymerase chain reaction (M-qPCR).

, 2015
Performance comparison of culture against multiplex real-time polymerase chain reaction (M-qPCR).
Charlotte Elizabeth Comé (798278), Inácio Mandomando (89296), Cícero Dias (652086), Tomás Francisco Zimba (798280), Eduardo Samo Gudo (353394), Alcides Moniz Munguambe (798277), Aquino Albino Nhantumbo (798274), Milton Ozório Moraes (202227), Cynthia Baltazar Semá (798281), Juliana Caireão (798276), Gabriela do Carmo Pinto (798279), Vlademir Vicente Cantarelli (798275)   +11 more
core   +1 more source

Application of real-time and multiplex PCR assays to study leaf blotch epidemics in barley [PDF]

, 2007
Leaf blotch, caused by Rhynchosporium secalis, was studied in a range of winter barley cultivars using a combination of traditional plant pathological techniques and newly developed multiplex and real-time polymerase chain reaction (PCR) assays.
Shaw, M.W., Ward, E., Fountaine, J. M., Shaw, M. W., Fraaije, B. A., Fountaine, J.M., Napier, B., Ward, E., Fraaije, B.A., Napier, B.   +9 more
core   +1 more source

Numerical and experimental study of a droplet-based PCR chip

, 2007
A two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface.
MacAskill, Alexandra, Goddard, N.J., Emerson, David, Macaskill A, Emerson DR, Day, P. J R, Fielden, P. R., Emerson, D. R., Zhang, Y. H., Mohr S, Day, Philip; id_orcid, Day PJR, Barber RW, Fielden, P.R., Barber, R. W., Zhang YH, Zhang, Yonghao, Goddard NJ, Goddard, N. J., Barber, Robert W., Macaskill, A., Mohr, S., Fielden PR, Day, P.J.R.   +23 more
core   +1 more source

A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea

BMC Infectious Diseases, 2021
Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work
Frieder Schaumburg, Neele Froböse, Robin Köck   +2 more
doaj   +1 more source

Measurement of Gene Expression by Multiplex Competitive Polymerase Chain Reaction

Analytical Biochemistry, 1993
We have developed a polymerase chain reaction (PCR)-based method to measure glutathione peroxidase (GSH-Px) mRNA levels. Expression was measured by multiplex competitive PCR amplification of (a) cDNA from GSH-Px and the "housekeeping" gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and (b) two internal standards consisting of single-base mutants ...
M J, Apostolakos, W H, Schuermann, M W, Frampton, M J, Utell, J C, Willey   +4 more
openaire   +2 more sources