Results 41 to 50 of about 3,481,864 (291)
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]
, 1992 Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
Dunham, Ian +7 more
core +1 more source
Biomédica: revista del Instituto Nacional de Salud, 2018 Introduction: Angiostrongyliasis is a disease caused by Angiostrongylus nematodes that is present worldwide. The infections with the highest impact on human and animal health are caused by A. cantonensis, A. costaricensis, and A. vasorum.
Rubén E. Varela-M +2 more
doaj +1 more source
Journal of Microbiology, Immunology and Infection, 2021 Background: Pertussis, is an infectious respiratory disease caused by Bordetella pertussis. The incidence of pertussis has been increasing in South Korea to due to waning vaccine-induced immunity. Culture has a low sensitivity and a long turnaround time (
Se Chang Oh +8 more
doaj +1 more source
A molecular approach (multiplex polymerase chain reaction) for diagnosis of rhinosporidiosis [PDF]
Indian Journal of Otolaryngology and Head and Neck Surgery, 2002 A New multiplex PCR have been developed in our laboratory using primer sets, aiming amplification of both D.N.A target fragments obtained in 18S RNA of commonly encountered fungi in human being and in Rhinosporidium seeberi using F1-fw/F2-rev (500 bp target) and Rhino-fw/ Rhino-rev (.177 bp target).
Somnath, Saha +5 more
openaire +2 more sources
MCMC-ODPR : primer design optimization using Markov Chain Monte Carlo sampling [PDF]
, 2012 Background Next generation sequencing technologies often require numerous primer designs that require good target coverage that can be financially costly.
Kitchen, James +7 more
core +1 more source
Forensic validation studies of a novel 35-InDel multiplex polymerase chain reaction system
Journal of Forensic Science and Medicine, 2023 Background: A difficulty associated with forensic applications is the detection of degraded biological materials. Due to the large amplicon sizes of short tandem repeat alleles, valid genotyping results cannot be obtained from degraded biological ...
Tong Xie +7 more
doaj +1 more source
Molecular Oncology, EarlyView.Detecting circulating tumor cells (CTCs) in blood before surgery may help predict outcomes in patients with head and neck squamous cell carcinoma (HNSCC). Here, we show when combined with tumor size and lymph node involvement from routine imaging, CTC status identifies high‐risk patients with poorer survival—offering a simple, minimally invasive tool ...
Susanne Flach +9 more
wiley +1 more source
Molecular Oncology, EarlyView.High‐risk bladder cancer is typically treated with Bacillus Calmette‐Guérin (BCG), but 30–40% of patients relapse. No FDA‐ or CE‐approved biomarkers currently predict or prognosticate BCG failure. We systematically reviewed the literature and identified 72 eligible studies, revealing several promising biomarkers associated with BCG treatment response ...
Rui Ribeiro‐Pereira +7 more
wiley +1 more source
A comparison of two multiplex-PCR assays for the diagnosis of traveller’s diarrhoea
BMC Infectious Diseases, 2021 Background Numerous multiplex-PCR assays are now available in routine diagnostics but their clinical value is controversial if a clear association between clinical symptoms and the detection of a particular pathogen is missing. The objective of this work
Frieder Schaumburg +2 more
doaj +1 more source
Molecular Oncology, EarlyView.We established the avian chorioallantoic membrane (CAM) assay as a scalable in vivo model for studying circulating tumor cells (CTCs). Human gastrointestinal tumors spontaneously released genetically validated CTCs that were detected across multiple platforms, demonstrating that the CAM model provides an accessible tool for investigating early cancer ...
Dennis Roth +17 more
wiley +1 more source