Results 41 to 50 of about 1,719,427 (359)

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using
V Sundeep Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
doaj   +1 more source

Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens

Poultry Science, 2022
: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella ...
Zhike Liu, Yan Yu, Tetiana Fotina, Roman Petrov, Zhanna Klishchova, Anatoliy Fotin, Jinyou Ma   +6 more
doaj   +1 more source

Evaluation of multiplex ligation-dependent probe amplification analysis versus multiplex polymerase chain reaction assays in the detection of dystrophin gene rearrangements in an Iranian population subset

Advanced Biomedical Research, 2014
Background: The Duchenne muscular dystrophy (DMD) gene is located in the short arm of the X chromosome (Xp21). It spans 2.4 Mb of the human genomic DNA and is composed of 79 exons.
Nayereh Nouri, Esmat Fazel-Najafabadi, Mansoor Salehi, Majid Hosseinzadeh, Mahdieh Behnam, Mohammad Reza Ghazavi, Maryam Sedghi   +6 more
doaj   +1 more source

Multiplex PCR system for the rapid diagnosis of respiratory virus infection: systematic review and meta-analysis

Clinical Microbiology and Infection, 2017
Objectives To provide a summary of evidence for the diagnostic accuracies of three multiplex PCR systems (mPCRs)—BioFire FilmArray RP (FilmArray), Nanosphere Verigene RV+ test (Verigene RV+) and Hologic Gen-Probe Prodesse assays—on the detection of viral
Huai-Shiuan Huang, Chi-Lin Tsai, Jeffrey S. Chang, T. Hsu, Samuel Lin, Samuel Lin, Chien-Chang Lee   +6 more
semanticscholar   +1 more source

Nanoliter Multiplex PCR Arrays on a SlipChip [PDF]

Analytical Chemistry, 2010
The SlipChip platform was tested to perform high-throughput nanoliter multiplex PCR. The advantages of using the SlipChip platform for multiplex PCR include the ability to preload arrays of dry primers, instrument-free sample manipulation, small sample volume, and high-throughput capacity.
Shen, Feng, Du, Wenbin, Davydova, Elena K., Karymov, Mikhail A., Pandey, Janmajay, Ismagilov, Rustem F.   +5 more
openaire   +3 more sources

Identification of the most common pathogenic bacteria in patients with suspected sepsis by multiplex PCR

Journal of Infection in Developing Countries, 2014
Introduction: Staphylococcus aureus, coagulase-negative staphylococci, Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumanii have been found to be the most prevalent bacteremia-causing bacteria in patients with ...
Mohammad Reza Arabestani, Hossein Fazzeli, Bahram Nasr Esfahani   +2 more
doaj   +1 more source

Applied genomics: data mining reveals species-specific malaria diagnostic targets more sensitive than 18S rRNA. [PDF]

, 2011
Accurate and rapid diagnosis of malaria infections is crucial for implementing species-appropriate treatment and saving lives. Molecular diagnostic tools are the most accurate and sensitive method of detecting Plasmodium, differentiating between ...
Jenna Oberstaller, Ganesh Srinivasamoorthy, Venkatachalam Udhayakumar, John W. Barnwell, Leopoldo Villegas, S. Patrick Kachur, Demas, Allison, Kissinger, Jessica C, Villegas, Leopoldo, Abdunoor M. Kabanywanyi, Jessica C. Kissinger, Deborah Sumari, Escalante, Ananias A, Lucchi, Naomi W, Udhayakumar, Venkatachalam, Sumari, Deborah, Peterson, David S, Ananias A. Escalante, Naomi W. Lucchi, Jeremy DeBarry, DeBarry, Jeremy, David S. Peterson, Oberstaller, Jenna, Allison Demas, Srinivasamoorthy, Ganesh, Kachur, S Patrick, Kabanywanyi, Abdunoor M, Barnwell, John W   +27 more
core   +1 more source

Development of a Multiplex PCR for Identification of Vineyard Mealybugs [PDF]

Environmental Entomology, 2011
A simple molecular tool was developed and tested to identify seven mealybug species found in North American vineyards: Pseudococcus maritimus Ehrhorn, Pseudococcus viburni (Signoret), Pseudococcus longispinus (Targioni-Tozzeti), Pseudococcus calceolariae (Maskell), Planococcus ficus (Signoret), Planococcus citri (Risso), and Ferrisia gilli Gullan.
Daane, K.M., Middleton, M.C., Sforza, R., Cooper, M.L., Walton, V.M., Walsh, D.B., Zaviezo Palacios, Tania, Almeida, R.P.P.   +7 more
openaire   +5 more sources

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction [PDF]

, 2008
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a ...
Aarts, H.J.M., AM Angeline Van Hoef, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Esther J Kok, Henriek G Beenen, Schoen, C.D., Beenen, H.G., Cor D Schoen, Henk JM Aarts, Marleen M Voorhuijzen, Hoef, A.M.A., van, Jeroen P van Dijk, Theo W Prins, Dijk, J.P., van, Prins, T.W., Voorhuijzen, M.M.   +17 more
core   +1 more source

EVALUATION OF MULTIPLEX PCR BASED TEST IN COMPARISON WITH CULTURE METHOD FOR DETECTION OF MYCOPLASMOSIS IN INFECTED CHICKENS [PDF]

Veterinary Medical Journal - Giza, 2011
A total of 94 chicken samples were examined for Mycoplasma gallisepticum and Mycoplasma synoviae either by culturing procedure or by using the multiplex PCR.
Salama S, Afaf Khedr, Shell S
doaj   +1 more source