Results 51 to 60 of about 144,579 (267)

A Multiplex RT-PCR Assay for S. aureus, L. monocytogenes, and Salmonella spp. Detection in Raw Milk with Pre-enrichment

Frontiers in Microbiology, 2017
This study firstly developed a multiplex real-time PCR (RT-PCR) technique combined with a pre-enrichment step to simultaneously detect Staphylococcus aureus (S. aureus), Listeria monocytogenes (L. monocytogenes) and Salmonella spp.
Tian Ding, Yuanjie Suo, Zhaohuan Zhang, Donghong Liu, Xingqian Ye, Shiguo Chen, Yong Zhao   +6 more
doaj   +1 more source

Compound Heterozygote Friedreich Ataxia Patients With Covert Proximal FXN Gene Deletions

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT We present Friedreich ataxia patients with frataxin gene deletions. Data and records were collected at the Children's Hospital of Philadelphia from patients enrolled in the FACOMS natural history study. Patients with proximal deletions initially diagnosed with only one GAA expanded allele had more severe disease than their homozygous expansion
Michael P. Lazaropoulos, Morgan C. Devore, Christina Lam, Courtney Park, Sanjay Bidichandani, David R. Lynch   +5 more
wiley   +1 more source

A novel XbaI multiplex PCR method for rapid typing of Klebsiella pneumoniae strains

Scientific Reports
This study focused on exploiting single-nucleotide polymorphism (SNP) variations in and around XbaI-restriction sites (5′….T↓CTAGA….3′) within the genomes of Klebsiella pneumoniae to develop a multiplex PCR genotyping technique that integrates the high ...
Ibrahim Ali Al-Zahrani
doaj   +1 more source

Multiplex PCR for molecular screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp.

Annals of Agricultural and Environmental Medicine, 2015
Introduction Ticks transmit a great variety of pathogenic microorganisms to humans and animals. The detection of tick-borne pathogens (TBP) is mainly by molecular techniques based on polymerase chain reactions (PCR).
Islay Rodríguez, Caroline Burri, Angel A. Noda, Véronique Douet, Lise Gern   +4 more
doaj   +1 more source

The Price of Precision: A Critical Review of Molecular Diagnostics in Glioma, From Guidelines to Global Disparities

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Gliomas have undergone a profound redefinition over the past decade, transitioning from morphology‐based entities to biologically coherent diseases defined by molecular alterations. The 2021 WHO Classification of Tumors of the Central Nervous System and its 2022 update formalize this shift, establishing integrated diagnosis as the global ...
Maria Guarnaccia, Sebastiano Cavallaro
wiley   +1 more source

Impact of Multiplex PCR on Diagnosis of Bacterial and Fungal Infections and Choice of Appropriate Antimicrobial Therapy

Diagnostics
Multiplex Polymerase Chain Reaction (PCR) has significantly impacted the field of infectious disease diagnostics, offering rapid and precise identification of bacterial and fungal pathogens.
Francesca Serapide, Rita Pallone, Angela Quirino, Nadia Marascio, Giorgio Settimo Barreca, Chiara Davoli, Rosaria Lionello, Giovanni Matera, Alessandro Russo   +8 more
doaj   +1 more source

Tuning Mechanoreceptor Signaling Enhances the Adjuvant Effect of Biomaterial Vaccines

Advanced Functional Materials, EarlyView.
Matrix stiffness serves as an independent immunological signal in biomaterial vaccines. Stiffer microporous hydrogel vaccines enhance dendritic cell maturation and migration to draining lymph nodes, promote T follicular helper cell expansion through Yap/Taz signaling, and increase antigen‐specific antibody production.
Lily Shang, Daniel P. Mayer, Prabhat K. Purbey, Yingzi Liu, Kindra Kelly‐Scumpia, Clara Kang, Natalie Tsubamoto, Dhriti Bolangadi, Iqra Arshad, Charisse Ng, Maksim V. Plikus, Dino Di Carlo, Jason S. Weinstein, Philip O. Scumpia   +13 more
wiley   +1 more source

Development of Multiplex PCR for Simultaneous Detection of Trypanosoma evansi and Equine Piroplasma Infection in Horses

Hayati Journal of Biosciences
The identification of equine piroplasmosis and surra disease in infected horses currently presents a significant challenge. Clinical symptoms and blood smears are commonly used to diagnose these two diseases. The objective of this study was to develop a
Arifin Nugraha, Umi Cahyaningsih
doaj   +1 more source

Multifunctional Catechol‐Functionalized Cellulose Hydrogels for the Minimally Invasive Treatment of Acute Optic Nerve Injuries

Advanced Healthcare Materials, EarlyView.
Catechol‐functionalized cellulose hydrogels are developed as injectable, bioadhesive platforms for retinal neuroprotection. The hydrogels exhibit tunable rheological and mechanical properties, strong tissue adhesion, and sustained antioxidative activity.
Kai‐Hsiang Chang, Yi‐Ke Lin, Wei‐Li Chen, Ta‐Ching Chen, Jiashing Yu   +4 more
wiley   +1 more source

Portable Molecularly Imprinted Polymer Sensor for Rapid Swab‐Based Detection of Norovirus

Advanced Healthcare Materials, EarlyView.
This work reports a rapid, portable smartphone‐based sensor capable of detecting four norovirus genotypes from swab samples within 15 min. Sensor selectivity was validated through evaluation against four common live viruses. The swab‐based sampling process enabled simple sample preparation combined with the compact detection system, supporting use of ...
Amy Dann, Pankaj Singla, Minji Kim, Sloane Stoufer, Clare Thomas, Robert D. Crapnell, Craig E. Banks, Jake McClements, Shayan Seyedin, Mark Geoghegan, Samuel T. Jones, Christopher F. Blanford, Matthew D. Moore, Marloes Peeters   +13 more
wiley   +1 more source