Results 51 to 60 of about 957,735 (260)

Successful gene expression analysis by multiplex, real-time, one-step RT-PCR, irrespective of the targets amplified

BioTechniques, 2007
Multiplex, real-time, one-step RT-PCR enables simultaneous quantification of several transcripts in one reaction vessel. This saves time and conserves precious sample material when analyzing the expression of reference and target genes in a particular ...
Holger Engel, Corinna Kueppers, Margaretha Koenig, Dirk Loeffert   +3 more
doaj   +1 more source

Comparison of Application of MD-VF-Auto SEM Method and Plankton Gene Multiplex PCR System in the Diagnosis of Drowning

Fayixue Zazhi, 2022
ObjectiveTo compare the application effect of microwave digestion - vacuum filtration - automated scanning electron microscopy （MD-VF-Auto SEM） method and plankton gene multiplex PCR system in the diagnosis of drowning.MethodsLung, liver and kidney ...
ZHANG Jian-miao, LIN Tian-chun, LIAO Zhen-yu, DU Yu-kun, YU Zhong-hao, LIU Jing-jian, WU Sai-qun, KANG Xiao-dong, XU Qu-yi, SHI He, ZHAO Jian, LIU Chao, GU Dang-en   +12 more
doaj   +1 more source

Optimised padlock probe ligation and microarray detection of multiple (non-authorised) GMOs in a single reaction [PDF]

, 2008
Background To maintain EU GMO regulations, producers of new GM crop varieties need to supply an event-specific method for the new variety. As a result methods are nowadays available for EU-authorised genetically modified organisms (GMOs), but only to a ...
Aarts, H.J.M., AM Angeline Van Hoef, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Esther J Kok, Henriek G Beenen, Schoen, C.D., Beenen, H.G., Cor D Schoen, Henk JM Aarts, Marleen M Voorhuijzen, Hoef, A.M.A., van, Jeroen P van Dijk, Theo W Prins, Dijk, J.P., van, Prins, T.W., Voorhuijzen, M.M.   +17 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Identification and Discrimination of Salmonella enterica Serovar Gallinarum Biovars Pullorum and Gallinarum Based on a One-Step Multiplex PCR Assay

Frontiers in Microbiology, 2018
Salmonella enterica serovar Gallinarum biovars Pullorum (S. Pullorum) and Gallinarum (S. Gallinarum) can result in pullorum disease and fowl typhoid in avian species, respectively, and cause considerable economic losses in poultry in many developing ...
Dan Xiong, Dan Xiong, Dan Xiong, Dan Xiong, Li Song, Li Song, Li Song, Li Song, Zhiming Pan, Zhiming Pan, Zhiming Pan, Zhiming Pan, Xinan Jiao, Xinan Jiao, Xinan Jiao, Xinan Jiao   +15 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Development of novel DNA marker for species discrimination of Fasciola flukes based on the fatty acid binding protein type I gene

Parasites & Vectors, 2022
Background Multiplex polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism (RFLP) for nuclear phosphoenolpyruvate carboxykinase (pepck) and polymerase delta (pold), respectively, have been used to differentiate Fasciola ...
Emi Okamoto, Michiyo Tashiro, Pedro Ortiz, Uday Kumar Mohanta, Cristian Hobán, César A. Murga-Moreno, José M. Angulo-Tisoc, Madoka Ichikawa-Seki   +7 more
doaj   +1 more source

Kesesuaian Uji Antigen Capture Enzyme Linked Immunosorbant Assay dan Nested Multiplex Polymerase Chain Reaction untuk Diagnosis Rutin Bovine Viral Diarrhea

Jurnal Ilmu Peternakan dan Veteriner Tropis, 2022
Bovine Viral Diarrhea (BVD) is one of the main causes of impaired productivity and reproduction of cows. Antigen capture Elisa (ACE) is one of the serological technique that is sensitive, reliable and used regularly for detecting persistent BVD infection
Sri Handayani Irianingsih, Dessie Eri Waluyati, Desi Puspita Sari, Hastari Wuryastuty   +3 more
doaj   +1 more source

Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation-detection assay [PDF]

, 2009
Simultaneous detection and identification of multiple pathogenic microorganisms in complex environmental samples are required in numerous diagnostic fields.
Dullemans, A.M., Slawiak, M., Dullemans, A., Van Doorn, R., Doorn, R., van, Kowalchuk, G.A., Doorn, R. van, Schoen, C.D., Szemes, M., Bonants, P.J.M., Bonants, P.   +10 more
core   +2 more sources

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source