Results 31 to 40 of about 1,719,427 (359)

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Multiplex PCR: Critical Parameters and Step-by-Step Protocol

BioTechniques, 1997
By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu, N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt   +4 more
doaj   +1 more source

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCR

BMC Bioinformatics, 2023
Background Multiplex PCR amplifies numerous targets in a single tube reaction and is essential in molecular biology and clinical diagnostics. One of its most important applications is in the targeted sequencing of pathogens.
Jason D. Limberis, John Z. Metcalfe
doaj   +1 more source

Multiplex Digital Methylation‐Specific PCR for Noninvasive Screening of Lung Cancer

Advancement of science, 2023
There remains tremendous interest in developing liquid biopsy assays for detection of cancer‐specific alterations, such as mutations and DNA methylation, in cell‐free DNA (cfDNA) obtained through noninvasive blood draws.
Yang Zhao, C. O’Keefe, K. Hsieh, L. Cope, Sonali C. Joyce, T. R. Pisanic, James G Herman, Tza-Huei Wang   +7 more
semanticscholar   +1 more source

Real-Time PCR and Multiplex Approaches [PDF]

, 2011
Analysis of RNA expression levels by real-time reverse-transcription (RT) PCR has become a routine technique in diagnostic and research laboratories. Monitoring of DNA amplification can be done using fluorescent sequence-specific probes, which generate signal only upon binding to their target.
Olga L, Gurvich, Mikhail, Skoblov
openaire   +2 more sources

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente

Perinatología y Reproducción Humana, 2023
Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos.
Marcos R. Escobedo-Guerra, Marcela López-Hurtado, Rodrigo Gutiérrez-Trujillo, Abraham D. Bustos-López, Fernando M. Guerra-Infante   +4 more
doaj   +1 more source

Development of a multiplex DNA-based traceability tool for crop plant materials [PDF]

, 2011
The authenticity of food is of increasing importance for producers, retailers and consumers. All groups benefit from the correct labelling of the contents of food products.
Theo W. Prins, Jeroen P. van Dijk, van Dijk, J.P., van Hoef, A.M.A., Kok, E.J., Ralf Seyfarth, Marleen M. Voorhuijzen, Seyfarth, R., A. M. Angeline Van Hoef, Hoef, A.M.A., van, Dijk, J.P., van, Voorhuijzen, M.M., Prins, T.W., Esther J. Kok   +13 more
core   +1 more source

MULTIPLEX PCR BASED URINARY TRACT INFECTION (UTI) ANALYSIS COMPARED TO TRADITIONAL URINE CULTURE IN IDENTIFYING SIGNIFICANT PATHOGENS IN SYMPTOMATIC PATIENTS.

Urology, 2019
OBJECTIVE To evaluate whether multiplex PCR-based molecular testing is non-inferior to urine culture for detection of bacterial infections in symptomatic patients.
K. Wojno, D. Baunoch, N. Luke, M. Opel, H. Korman, C. Kelly, S. Mohammad A. Jafri, P. Keating, D. Hazelton, S. Hindu, Bridget Makhloouf, David L. Wenzler, M. Sabry, F. Burks, Miguel Penaranda, D. E. Smith, A. Korman, L. Sirls   +17 more
semanticscholar   +1 more source

Primer1: Primer design Web service for Tetra-Primer ARMS-PCR [PDF]

, 2012
Tetra-primer ARMS-PCR is used extensively as a low cost, single PCR assay requiring no post-PCR manipulation. The design of successful primers depends on a number of variables such as melting temperatures, GC content, complementarity and selection of ...
Ke, Xiayi, Collins, Andrew
core   +1 more source