Results 11 to 20 of about 1,719,427 (359)

Multiplex PCR for molecular screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp.

Annals of Agricultural and Environmental Medicine, 2015
Introduction Ticks transmit a great variety of pathogenic microorganisms to humans and animals. The detection of tick-borne pathogens (TBP) is mainly by molecular techniques based on polymerase chain reactions (PCR).
Islay Rodríguez, Caroline Burri, Angel A. Noda, Véronique Douet, Lise Gern   +4 more
doaj   +2 more sources

Establishment of a Multiplex RT-PCR Method for the Detection of Five Known Genotypes of Porcine Astroviruses

Frontiers in Veterinary Science, 2021
Porcine astroviruses (PAstVs) are prevalent in pigs worldwide, and five genotypes have been reported to circulate in China. However, little is known about the coinfection status of PAstVs.
Xin Liu, Wenchao Zhang, Dongjing Wang, Xinyue Zhu, Ying Chen, Kang Ouyang, Zuzhang Wei, Huan Liu, Weijian Huang   +8 more
doaj   +1 more source

Multicenter evaluation of a syndromic rapid multiplex PCR test for early adaptation of antimicrobial therapy in adult patients with pneumonia

Critical Care, 2020
Background Improving timeliness of pathogen identification is crucial to allow early adaptation of antibiotic therapy and improve prognosis in patients with pneumonia.
Céline Monard, Jonathan Pehlivan, G. Auger, S. Alviset, A. Tran Dinh, Paul Duquaire, N. Gastli, C. d'Humières, A. Maamar, A. Boibieux, M. Baldeyrou, J. Loubinoux, O. Dauwalder, V. Cattoir, L. Armand-Lefèvre, S. Kernéis, Agathe Julien Julien Claude-Alexandre Grégory François Br Becker Charpentier Textoris Gustave Destras Vanden, A. Becker, J. Charpentier, J. Textoris, Claude-Alexandre Gustave, G. Destras, F. Vandenesch, B. Lina, J. Casalegno, M. Lejeune, P. Montravers, C. Poyart, H. Tête, J. Timsit, Thomas Uberti   +30 more
semanticscholar   +1 more source

Improvements to the ARTIC multiplex PCR method for SARS-CoV-2 genome sequencing using nanopore

bioRxiv, 2020
Genome sequencing has been widely deployed to study the evolution of SARS-CoV-2 with more than 90,000 genome sequences uploaded to the GISAID database.
J. R. Tyson, P. James, D. Stoddart, N. Sparks, Arthur Wickenhagen, G. Hall, Ji‐Hyun Choi, H. Lapointe, K. Kamelian, Andrew D. Smith, N. Prystajecky, I. Goodfellow, Sam J. Wilson, R. Harrigan, T. Snutch, N. Loman, J. Quick   +16 more
semanticscholar   +1 more source

Selecting a multiplex PCR panel for accurate molecular diagnosis of intestinal protists: a comparative study of Allplex® (Seegene®), G-DiaParaTrio (Diagenode®), and RIDA®GENE (R-Biopharm®) assays and microscopic examination

Parasite, 2022
Commercial multiplex PCR assay panels were developed to overcome the limitations of microscopic examination for parasitological diagnosis on stool samples. However, given the increased supply of this diagnostic approach, these assays must be evaluated to
Argy Nicolas, Nourrisson Céline, Aboubacar Ahmed, Poirier Philippe, Valot Stéphane, Laude Adrien, Desoubeaux Guillaume, Pomares Christelle, Machouart Marie, Le Govic Yohann, Dalle Frédéric, Botterel Françoise, Bourgeois Nathalie, Cateau Estelle, Leterrier Marion, Le Pape Patrice, Morio Florent, Houze Sandrine   +17 more
doaj   +1 more source

Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples

Nature Protocols, 2017
Genome sequencing has become a powerful tool for studying emerging infectious diseases; however, genome sequencing directly from clinical samples (i.e., without isolation and culture) remains challenging for viruses such as Zika, for which metagenomic ...
J. Quick, N. Grubaugh, S. Pullan, I. Claro, Andrew D. Smith, K. Gangavarapu, G. Oliveira, R. Robles-Sikisaka, T. Rogers, N. Beutler, D. Burton, L. Lewis-Ximenez, J. D. de Jesus, M. Giovanetti, S. Hill, Allison Black, T. Bedford, M. Carroll, M. Nunes, L. Alcântara, E. Sabino, S. Baylis, N. Faria, M. Loose, J. Simpson, O. Pybus, Kristian G. Andersen, N. Loman   +27 more
semanticscholar   +1 more source

Evaluation of the QIAstat-Dx Respiratory SARS-CoV-2 Panel, the First Rapid Multiplex PCR Commercial Assay for SARS-CoV-2 Detection

Journal of Clinical Microbiology, 2020
In the race to contain severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), efficient detection and triage of infected patients must rely on rapid and reliable testing.
B. Visseaux, Q. Le Hingrat, G. Collin, D. Bouzid, S. Lebourgeois, Diane le Pluart, L. Deconinck, F. Lescure, J. Lucet, L. Bouadma, J. Timsit, D. Descamps, Y. Yazdanpanah, E. Casalino, N. Houhou-Fidouh   +14 more
semanticscholar   +1 more source

Multiplex PCR Assay for unequivocal differentiation of Actinobacillus pleuropneumoniae serovars 1 to 3, 5 to 8, 10, and 12 [PDF]

, 2014
This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G003203/1) and the United Kingdom Department for Environment, Food
Maskell, Duncan J, Holden, M T, Chaudhuri, RR, Tucker, AW, Rycroft, Andrew N, Rycroft, AN, Angen, O, Tucker, A W, Weinert, LA, Weinert, Lucy A, Wren, Brendan W, Cambridge, Holden, Matthew T, Bossé, J T, BRaDP1T Consortium, LSHTM, Chaudhuri, R R, Bossé, Janine T, Angen, Oystein, Tucker, Alexander W, Norwegian Vet Instit, Chaudhuri, Roy R, Langford, PR, Holden, MT, Angen, Øystein, Rycroft, A N, Bosse, JT, Holden, Matt T, Wellcome Trust Sanger Institute, Wren, BW, Imperial College London, Williamson, Susanna M, Williamson, S M, Maskell, D J, Wren, B W, Williamson, SM, Weinert, L A, Li, Yanwen, Langford, P R, AHVLA, Maskell, DJ, Langford, Paul R, Holden, Matthew, Li, Y, Angen, Ø   +44 more
core   +1 more source

Two-color quantitative multiplex methylation-specific PCR

BioTechniques, 2006
In recent years, several methylation-specific PCR-based techniques have been developed to identify and characterize hypermethylation of CpG dinucleotides with the primary goal of elucidating a better understanding of the role of DNA methylation in ...
Theresa Swift-Scanlan, Amanda Blackford, Pedram Argani, Saraswati Sukumar, Mary Jo Fackler   +4 more
doaj   +1 more source

Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples. [PDF]

, 2008
peer-reviewedBackground: A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium, Kentucky and ...
Sheila McGuinness, McCabe, Evonne, Paul Whyte, Geraldine Duffy, McGuinness, Sheila, Barry, Thomas, Thomas Barry, Edel O'Regan, Séamus Fanning, Duffy, Geraldine, Whyte, Paul, O'Regan, Edel, Evonne McCabe, Catherine Burgess, Burgess, Catherine, Fanning, Seamus   +15 more
core   +1 more source