Results 11 to 20 of about 144,579 (267)

Development of multiplex PCR for rapid detection of metallo-β-lactamase genes in clinical isolates of Acinetobacter baumannii

Iranian Journal of Microbiology, 2020
Background and Objectives: Acinetobacter baumannii has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical ...
Reza Ranjbar, Shahin Zayeri, Amir Mirzaie   +2 more
doaj   +1 more source

Molecular diagnosis of the human immunodeficiency, Hepatitis B and C viruses among blood donors in Lomé (Togo) by multiplex real time PCR

The Pan African Medical Journal, 2016
This study aimed to compare the sensitivity of multiplex PCR to ELISA technique in the instantaneous detection of HBV, HCV and HIVin blood samples from donors of the National blood Transfusion Centre in Togo.
Maleki Assih, Lochina Feteke, Cyrille Bisseye, Djeneba Ouermi, Florencia Djigma, Simplice Damintoti Karou, Jacques Simpore   +6 more
doaj   +1 more source

Multiplex PCR: Critical Parameters and Step-by-Step Protocol

BioTechniques, 1997
By simultaneously amplifying more than one locus in the same reaction, multiplex PCR is becoming a rapid and convenient screening assay in both the clinical and the research laboratory.
O. Henegariu, N.A. Heerema, S.R. Dlouhy, G.H. Vance, P.H. Vogt   +4 more
doaj   +1 more source

Development of a Multiplex Crystal Digital RT-PCR for Differential Detection of Classical, Highly Pathogenic, and NADC30-like Porcine Reproductive and Respiratory Syndrome Virus

Animals, 2023
Porcine reproductive and respiratory syndrome virus (PRRSV) type 1 (European genotype) and PRRSV type 2 (North American genotype) are prevalent all over the world.
Feng Long, Yating Chen, Kaichuang Shi, Yanwen Yin, Shuping Feng, Hongbin Si   +5 more
doaj   +1 more source

Fragment Size Difference between Multiplex and Singleplex PCR Products and Their Practical Implications

BioTechniques, 2000
By simultaneously amplifying several loci in the same reaction, multiplex PCR has been used in gene mapping and DNA typing with polymorphic short tandem repeat loci.
H.-W. Deng, Y. Zhou, R.R. Recker, M.L. Johnson, J. Li   +4 more
doaj   +1 more source

Optimizations of high throughput multiplex polymerase chain reaction with simple sequence repeat markers for genotyping of common walnut populations (Juglans regia L.)

Silva Fennica, 2016
Multiplex polymerase chain reaction (PCR) allows amplification of two or more pair of primers in parallel for amplification of multiple target sequences in a single reaction tube.
Nevenka Ćelepirović, Monika Karija Vlahović, Aikaterini Dounavi, Mladen Ivanković   +3 more
doaj   +1 more source

Multiplex PCR assay based on the citE2 gene and intergenic sequence for the rapid detection of Salmonella Pullorum in chickens

Poultry Science, 2022
: Salmonella is one of the most common Gram-negative pathogens and seriously threatens chicken farms and food safety. This study aimed to establish a multiplex polymerase chain reaction (PCR) approach for the identification of different Salmonella ...
Zhike Liu, Yan Yu, Tetiana Fotina, Roman Petrov, Zhanna Klishchova, Anatoliy Fotin, Jinyou Ma   +6 more
doaj   +1 more source

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente

Perinatología y Reproducción Humana, 2023
Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos.
Marcos R. Escobedo-Guerra, Marcela López-Hurtado, Rodrigo Gutiérrez-Trujillo, Abraham D. Bustos-López, Fernando M. Guerra-Infante   +4 more
doaj   +1 more source

Analysis of a novel multiplex polymerase chain reaction assay as a sensitive tool for the diagnosis of indeterminate and tuberculoid forms of leprosy

International Journal of Mycobacteriology, 2017
Objective/Background: Clinical diagnosis of indeterminate and tuberculoid leprosy is often difficult due to limited and confounding signs and symptoms. In the current study, we evaluated the utility of new multiplex polymerase chain reaction (PCR) using
V Sundeep Chaitanya, Luz Cuello, Madhusmita Das, A Sudharsan, Pavithrra Ganesan, K Kanmani, Lakshmi Rajan, Mannam Ebenezer   +7 more
doaj   +1 more source

Fundamentals of multiplexing with digital PCR

Biomolecular Detection and Quantification, 2016
Over the past decade numerous publications have demonstrated how digital PCR (dPCR) enables precise and sensitive quantification of nucleic acids in a wide range of applications in both healthcare and environmental analysis. This has occurred in parallel with the advances in partitioning fluidics that enable a reaction to be subdivided into an ...
Whale, Alexandra S., Huggett, Jim F., Tzonev, Svilen   +2 more
openaire   +2 more sources