Results 211 to 220 of about 18,173,972 (253)

Bovine tuberculosis in Central Ethiopian slaughterhouses and the identification of causative mycobacteria by multiplex real-time PCR. [PDF]

BMC Microbiol
Fromsa A, Conlan AJK, Srinivasan S, Zeleke M, Worku D, Lakew M, Abdela MG, Bahiru G, Wood JLN, Bakker D, Gumi B, Ameni G, Kapur V.   +12 more
europepmc   +1 more source

Chemotherapy‐Activated GSK3β‐DNMT1 Signaling Upregulates CD47 to Evade Macrophage Phagocytosis and Drive Temozolomide Resistance in Glioblastoma

Advanced Science, EarlyView.
Temozolomide treatment activates GSK3β, driving DNMT1 phosphorylation, destabilization, and CD47 promoter hypomethylation in glioblastoma. This epigenetic shift upregulates CD47, enabling TMZ‐treated GBM cells to evade macrophage phagocytosis, survive chemotherapy, and acquire resistance.
Jie Li, Shuai Ying, Yi Zhang, Jingjing Wang, Quan Wan, Lingli Gong, Daxing Xu, Zhenkun Yang, Hongnian Lu, Ying Yin, Xiangming Fang, Zhening Pu   +11 more
wiley   +1 more source

Performance Assessment of a Multiplex Real-Time PCR Assay for Detection of Viruses Causing Respiratory Tract Infections. [PDF]

Diagnostics (Basel)
Upadhyay P, Surur F, Singh V.
europepmc   +1 more source

Optimized Cas9‐Enriched Nanopore Sequencing and Analysis Workflow for Clinical Diagnosis of Repeat Expansion Disorders

Advanced Science, EarlyView.
An optimized Cas9‐enriched nanopore sequencing workflow, combined with STRiker, enables simultaneous analysis of disease‐associated STR loci from patient blood. The nCATS–STRiker workflow detects repeat expansions, de novo repeat motifs, interruption patterns, and methylation in a single assay, improving the genetic diagnosis of previously undiagnosed ...
Seungbok Lee, Chanju Jung, Minjeong Kim, Narae Kim, Gue‐Ho Hwang, Soon‐Tae Lee, Kon Chu, Sang Kun Lee, Han‐Joon Kim, Jong‐Hee Chae, Sangsu Bae, Jangsup Moon   +11 more
wiley   +1 more source

Development of a multiplex real-time PCR for the simultaneous detection of monkeypox virus clades I, II, and goatpox virus. [PDF]

Front Vet Sci
Lin Y, Guo Z, Chen J, Zhang X, Zhou L, Li Y, Zhang Z.   +6 more
europepmc   +1 more source

A Dual‐Mode In Situ EV‐miRNA Profiling Platform for Machine‐Learning‐Assisted Gastric Cancer Liquid Biopsy

Advanced Science, EarlyView.
An in situ dual‐mode platform integrates fusogenic liposomes with localized autocatalytic DNA circuits for plasma‐derived extracellular vesicle microRNA analysis. Following membrane fusion, target miRNAs trigger feedback‐assisted LECHA amplification inside intact vesicles, generating fluorescence and electrochemical outputs. Machine learning integrates
Wenbin Li, Rui Fan, Kun Xie, Jiehua Zhong, Yitong Zhu, Tingting Ji, Yuanyuan Qin, Shijin Peng, Yu Zhang, Yuhang Guo, Tiange Zhang, Chunchen Liu, Bo Li, Lei Zheng, Ye Zhang, Xiaohui Yan   +15 more
wiley   +1 more source

Kogene PowerChek Multiplex Real-time PCR Kits Versus the BioFire FilmArray Gastrointestinal Panel: Roles of Crossing Point Values and Melting Curves in Interpreting the FilmArray Gastrointestinal Panel. [PDF]

Ann Lab Med
Yoo IY, Jo S, Kwon JA, Han JH, Lee HK, Park YJ.   +5 more
europepmc   +1 more source

Single‐Molecule Imaging of Endogenous RNA Using a Miniaturized and Circularly Permuted CRISPR‐Cas13 Degron‐Masking System CDegSR

Advanced Science, EarlyView.
CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley   +1 more source

Establishment and application of a one-step multiplex real-time PCR assay for detection of A, B, and C subtypes of avian metapneumovirus. [PDF]

Poult Sci
Su M, Cheng J, Xu X, Liu Y, Zhao Y, Wang Y, Du X, Ying J, Yan J, Zheng H, Cheng C, Sun J.   +11 more
europepmc   +1 more source

Nrsn1–Smarcc1 Coupling Regulates Neural Stem Cell Differentiation and Chronic‐phase Recovery After Ischemic Stroke

Advanced Science, EarlyView.
In the post‐stroke brain, Foxa2 induces the transcriptional upregulation of Nrsn1 in NSCs. Nrsn1 functionally couples with Smarcc1, modulating its nuclear availability and protein abundance, thereby influencing Smarcc1‐associated regulatory programs linked to neuronal lineage commitment. Through this coupling, Nrsn1 promotes the differentiation of NSCs
Ruolin Zhang, Chang Liu, Yuneng Zhou, Kaichen Zhao, Muyang Li, Bingcheng Cai, Ying Wang, Zhiyuan Yuan, Zhaoxin Liu, Zilong Yuan, Yao Xiao, Peiyang Zhou, Ke Shui, Wendai Bao, Min Zhang, Jun Qin, Jun Chen, Xin Yang, Zhiqiang Dong   +18 more
wiley   +1 more source