Doubling multiplexed imaging capability via spatial expression pattern-guided protein pairing and computational unmixing [PDF]
Communications BiologyThree-dimensional multiplexed fluorescence imaging is an indispensable technique in neuroscience. For two-dimensional multiplexed imaging, cyclic immunofluorescence, which involves repeating staining, imaging, and signal removal over multiple cycles, has
Gyuri Kim +5 more
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A perspective on FAIR quality control in multiplexed imaging data processing [PDF]
Frontiers in BioinformaticsMultiplexed imaging approaches are getting increasingly adopted for imaging of large tissue areas, yielding big imaging datasets both in terms of the number of samples and the size of image data per sample.
Wouter-Michiel A. M. Vierdag +1 more
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Delineating spatial cell-cell interactions in the solid tumour microenvironment through the lens of highly multiplexed imaging [PDF]
Frontiers in Immunology, 2023 The growth and metastasis of solid tumours is known to be facilitated by the tumour microenvironment (TME), which is composed of a highly diverse collection of cell types that interact and communicate with one another extensively.
David E. Cohn +9 more
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Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging [PDF]
Frontiers in ImmunologyDeciphering cellular components and the spatial interaction network of the tumor immune microenvironment (TIME) of solid tumors is pivotal for understanding biologically relevant cross-talks and, ultimately, advancing therapies.
Sophia Scheuermann +12 more
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cytoviewer: an R/Bioconductor package for interactive visualization and exploration of highly multiplexed imaging data [PDF]
BMC BioinformaticsBackground Highly multiplexed imaging enables single-cell-resolved detection of numerous biological molecules in their spatial tissue context. Interactive visualization of multiplexed imaging data is crucial at any step of data analysis to facilitate ...
Lasse Meyer +2 more
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Temporally multiplexed imaging of dynamic signaling networks in living cells [PDF]
Cell, 2023 Yong Qian, Zeguan Wang, Edward Boyden
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Frontiers in Immunology, 2022 Highly multiplexed, single-cell imaging has revolutionized our understanding of spatial cellular interactions associated with health and disease. With ever-increasing numbers of antigens, region sizes, and sample sizes, multiplexed fluorescence imaging ...
Guolan Lu +19 more
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Strategies for Accurate Cell Type Identification in CODEX Multiplexed Imaging Data
Frontiers in Immunology, 2021 Multiplexed imaging is a recently developed and powerful single-cell biology research tool. However, it presents new sources of technical noise that are distinct from other types of single-cell data, necessitating new practices for single-cell ...
John W. Hickey +7 more
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SnapFISH: a computational pipeline to identify chromatin loops from multiplexed DNA FISH data
Nature Communications, 2023 Multiplexed DNA fluorescence in situ hybridization (FISH) imaging technologies have been developed to map the folding of chromatin fibers at tens of nanometers and up to several kilobases in resolution in single cells.
Lindsay Lee +14 more
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Multiplexed structured imaging of laser induced fluorescence at 10 GHz rate
AIP Advances, 2023 The frame rate of conventional high-speed imaging devices is limited by exposure time and signal read-out time. Structured imaging is able to push the frame rate beyond the limit of the imaging device.
Zichen He +4 more
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