Results 111 to 120 of about 799 (137)

Expression of the myotonin protein kinase gene in preimplantation human embryos

Human Molecular Genetics, 1995
We have examined the expression of the myotonin protein kinase gene (MPK) in individual human preimplantation embryos. An allele-specific, reverse transcriptase polymerase chain reaction assay was developed to the required sensitivity using human buccal cells.
R, Daniels, T, Kinis, P, Serhal, M, Monk
openaire   +2 more sources

Expanded CTG repeats in myotonin protein kinase increase susceptibility to oxidative stress

NeuroReport, 1998
The effect of oxidative stress on myogenic cells with expanded CTG repeats in the myotonin protein kinase (MtPK) gene was investigated using MtPK cDNA-transformants in order to investigate the disease process underlying myotonic dystrophy. We employed methylmercury as a model for reagents that produce reactive oxygen species (ROS).
F, Usuki, S, Ishiura
openaire   +2 more sources

The Myotonin-Protein Kinase Phosphorylates Tyrosine Residues in Normal Human Skeletal Muscle

Biochemical and Biophysical Research Communications, 1994
As a first approach to study the cellular events involved in myotonic dystrophy, we have produced a polyclonal antibody against a peptide sequence of the predicted gene product. This antibody specifically recognizes a 54 kDa protein in human skeletal muscle. This protein phosphorylates a co-polymer Glu/Tyr but not Myelin Basic Protein.
P, Etongué-Mayer, R, Faure, J P, Bouchard, M C, Thibault, J, Puymirat   +4 more
openaire   +2 more sources

Decreased Myotonin-Protein Kinase in the Skeletal and Cardiac Muscles in Myotonic Dystrophy

Biochemical and Biophysical Research Communications, 1994
To investigate the role of myotonin-protein kinase (MT-PK) in the pathophysiology of myotonic dystrophy (DM), we developed specific antibodies against synthetic MT-PK peptides. The antibody identified a 53kDa protein in skeletal muscle and recognized decreases in the amount of the protein in both adult and congenital DM patients, compared with amounts ...
R, Koga, Y, Nakao, Y, Kurano, T, Tsukahara, A, Nakamura, S, Ishiura, I, Nonaka, K, Arahata   +7 more
openaire   +2 more sources

Immunocytochemical localization of a full-length myotonin protein kinase in rat L6 myoblasts

Neuroscience Letters, 1996
Expansion mutation of CTG-repeat motifs within myotonin protein kinase (MtPK) gene is responsible for pathological changes in myotonic dystrophy (DM). To explore its pathological role in skeletal muscle, a full-length human MtPK cDNA was transfected into rat L6 myogenic cell line.
N, Saitoh, N, Sasagawa, H, Koike, M, Shimokawa, H, Sorimachi, S, Ishiura, K, Suzuki   +6 more
openaire   +2 more sources

Myotonic Dystrophy: Decreased Levels of Myotonin Protein Kinase (Mt-PK) Leads to Apoptosis in Muscle Cells

Experimental Neurology, 1997
The pathogenesis of myotonic dystrophy (DM) and the function of the product of the DM gene, myotonin protein kinase (Mt-PK), and its relationship to the disease are uncertain. To gain insight into the function of Mt-PK we studied the effect of decreasing the levels of Mt-PK in cultured human myoblasts.
S, Bhagavati, S, Bhagwati, B, Leung, S A, Shafiq, A, Ghatpande   +4 more
openaire   +2 more sources

Decreased Expression of Myotonin-Protein Kinase Messenger RNA and Protein in Adult Form of Myotonic Dystrophy

Science, 1993
The myotonic dystrophy mutation has recently been identified; however, the molecular mechanism of the disease is still unknown. The sequence of the myotonin-protein kinase gene was determined, and messenger RNA spliced forms were identified in various tissues. Antisera were developed for analytical studies. Quantitative reverse transcription-polymerase
Ying-Hui Fu, David L. Friedman, Stephen Richards, Joel A. Pearlman, Richard A. Gibbs, Antonio Pizzuti, Tetsuo Ashizawa, M. Benjamin Perryman, Guglielmo Scarlato, Raymond G. Fenwick, C. Thomas Caskey   +10 more
openaire   +2 more sources

Differential Signaling Pathways Following Oxidative Stress in Mutant Myotonin Protein Kinase cDNA-Transfected C2C12 Cell Lines

Biochemical and Biophysical Research Communications, 2000
We investigated the response to oxidative stress in a model system established in C2C12 cells stably transfected with myotonin protein kinase (MtPK) cDNAs having 5, 46, 60, or 160 CTG repeats. The transformants showed CTG repeat number-dependent susceptibility to oxidative stress.
F, Usuki, N, Takahashi, N, Sasagawa, S, Ishiura   +3 more
openaire   +2 more sources

[Myotonin protein kinase].

Rinsho shinkeigaku = Clinical neurology, 1996
The mutation underlying myotonic dystrophy is the expansion of polymorphic CTG repeat in the 3'-noncoding region of the myotonin protein kinase (MtPK) gene mapping to chromosome 19q13.3. A full-length cDNA of human MtPK was cloned and expressed in COS-1 cells.
S, Ishiura, N, Sasagawa, N, Saitoh, H, Koike, H, Sorimachi, K, Suzuki, M, Shimokawa, F, Usuki, H, Nakase, K, Kamakura   +9 more
openaire   +1 more source

Different Expression of the Myotonin Protein Kinase Gene in Discrete Areas of Human Brain

Biochemical and Biophysical Research Communications, 1995
We have examined the myotonin protein kinase (MT-PK) gene expression in different human brain areas. Four different spliced forms, comprising exons 13 and 14, were identified and characterized. One form (MYOT-A), lacking the entire exon 13, had not been detected in other studies and it is likely to be a brain-specific transcript.
GENNARELLI M., LUCARELLI, Marco, ZELANO G., PIZZUTI, Antonio, NOVELLI G., DALLAPICCOLA B.   +5 more
openaire   +4 more sources