Results 241 to 250 of about 9,148 (283)
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Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Myrosinases (EC 3.2.3.1) are glucosinolate (thioglucoside) hydrolases primarily occurring in plants of the Cruciferae family. 1. 1.|The separation of three myrosinase isoenzymes in Sinapis alba seed was achieved by DEAE-cellulose chromatography on Whatman DE-52. 2.
Jan-Christer Janson, Rune Björkman
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Myrosinases (EC 3.2.3.1) are glucosinolate (thioglucoside) hydrolases primarily occurring in plants of the Cruciferae family. 1. 1.|The separation of three myrosinase isoenzymes in Sinapis alba seed was achieved by DEAE-cellulose chromatography on Whatman DE-52. 2.
Jan-Christer Janson, Rune Björkman
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Characterization of rapeseed myrosinase-binding protein
Planta, 1995Myrosinase-binding proteins (MBPs) were purified from seeds of Brassica napus L. (oilseed rape). The proteins were characterized with respect to amino-acid composition, peptide sequence and isoelectric points. Gel electrophoresis and Western blotting of protein extracts from mature seeds showed the existence of at least ten proteins reacting with a ...
Lars Rask +4 more
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Myrosinase Activity in Soil Extracts
Soil Science Society of America Journal, 1996AbstractMyrosinase (β‐thioglucoside glucohydrolase; EC 3.2.3.1) catalyzes the hydrolysis of glucosinolates to form a variety of potential allelochemicals. Although these allelochemicals may exert an influence on soil‐borne organisms, the extracellular preservation of plant‐derived myrosinase in soil has not been determined. Soil samples from a field of
Vladimir Borek +2 more
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Myrosinase activity of cruciferous vegetables
Journal of the Science of Food and Agriculture, 1984AbstractMyrosinase activity in partially purified extracts of 12 cruciferous vegetables and an acetone powder preparation of Sinapis alba L. (white mustard) was determined by the initial rate of glucose formation from glucosinolate hydrolysis using a coupled assay. Of the species studied Raphanus sativus L.
Michael J. C. Rhodes +2 more
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Plant Science, 2001
A method for analyzing the tissue distribution of myrosinase activity in Brassicaceous plants was developed. This technique is based on 'tissue printing' to visualize enzyme activity. The freshly-cut surface (transverse direction) of the root of three species, Japanese radish (Raphanus sativus), turnip (Brassica campestris) and Japanese horseradish ...
Hideo Eto, Toru Kuboi, Masakazu Hara
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A method for analyzing the tissue distribution of myrosinase activity in Brassicaceous plants was developed. This technique is based on 'tissue printing' to visualize enzyme activity. The freshly-cut surface (transverse direction) of the root of three species, Japanese radish (Raphanus sativus), turnip (Brassica campestris) and Japanese horseradish ...
Hideo Eto, Toru Kuboi, Masakazu Hara
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Biochimica et Biophysica Acta (BBA) - Enzymology, 1973
Abstract Myrosinases (EC 3.2.3.1) are a group of isoenzymes present in Cruciferaes which catalyze the hydrolysis of glucosinolates. The myrosinases from rapeseed (Brassica napus L.) have been purified and the main component isolated and characterized.
Jan Christer Janson, Bo Lönnerdal
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Abstract Myrosinases (EC 3.2.3.1) are a group of isoenzymes present in Cruciferaes which catalyze the hydrolysis of glucosinolates. The myrosinases from rapeseed (Brassica napus L.) have been purified and the main component isolated and characterized.
Jan Christer Janson, Bo Lönnerdal
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Comparison of Myrosinase Activity Assays
1987Plant thioglucoside glucohydrolase (myrosinase EC 3.2.3.1) catalyzes the hydrolysis of glucosinolates to isothiocyanate, glucose and sulfate. We have purified myrosinase from white mustard seed (Sinapis alba) in high yields and with a considerable specific activity in a single step by affinity chromatography on Con-A Sepharose.
Sandro Palmieri +2 more
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Characterization of a new myrosinase in Brassica napus
Plant Molecular Biology, 1995A full-length cDNA clone defining the new myrosinase gene family MC in Brassica napus was isolated and sequenced. Southern hybridization showed that the MC family probably consists of 3 or 4 genes in B. napus. MC genes are expressed in the developing seed, but not in the vegetative tissues investigated. In situ hybridizations to developing seeds showed
Bo Ek, Anders Falk, Lars Rask
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Functional Plant Biology, 2020
Clubroot is a devastating disease of Brassicaceae caused by the biotrophic protist Plasmodiophora brassicae. The progression of clubroot disease is modulated by the glucosinolate (GSL) profile of the host plant. GSL is hydrolysed by the enzyme myrosinase
M. Kayum +6 more
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Clubroot is a devastating disease of Brassicaceae caused by the biotrophic protist Plasmodiophora brassicae. The progression of clubroot disease is modulated by the glucosinolate (GSL) profile of the host plant. GSL is hydrolysed by the enzyme myrosinase
M. Kayum +6 more
semanticscholar +1 more source
The glucosinolate–myrosinase system in an ecological and evolutionary context
Current Opinion in Plant Biology, 2005Functional analysis of natural variation in the model species Arabidopsis thaliana has enabled the cloning of many glucosinolate biosynthesis and hydrolysis genes. Variation in these genes is central to understanding the ecological role of the glucosinolate-myrosinase defense system, and allows us to dissect the evolutionary and ecological forces that ...
Kliebenstein, Dan +2 more
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