Results 301 to 310 of about 13,066,219 (329)

Multiplexing N‐glycan analysis by DNA analyzer

ELECTROPHORESIS, 2017
Analysis of N‐glycan structures has been gaining attentions over the years due to their critical importance to biopharma‐based applications and growing roles in biological research. Glycan profiling is also critical to the development of biosimilar drugs.
Hua‐tao Feng, Pingjing Li, Guo Rui, James Stray, Shaheer Khan, Shiaw‐Min Chen, Sam F. Y. Li   +6 more
openaire   +2 more sources

The role of N-glycans in spermatogenesis

Cytogenetic and Genome Research, 2003
Many proteins, in particular those in the plasma membranes, are glycosylated with carbohydrates, which are grouped into O-glycans and N-glycans. O-glycans are synthesized step by step by glycosyltransferases, whereas N-glycans are synthesized by en-bloc transfer of the so-called high-mannose-type oligosachharide from lipid-linked precursor to ...
M N, Fukuda, T O, Akama
openaire   +2 more sources

N-glycan occupancy of Arabidopsis N-glycoproteins

Journal of Proteomics, 2013
Most secreted proteins in eukaryotes are modified on the amino acid consensus sequence NxS/T by an N-glycan through the process of N-glycosylation. The N-glycans on glycoproteins are processed in the endoplasmic reticulum (ER) to different mannose-type N-glycans or, when the protein passes through the Golgi apparatus, to different complex glycan forms.
Song, W., Mentink, R., Henquet, M.G.L., Cordewener, J.H.G., van Dijk, A.D.J., Bosch, H.J., America, A.H.P., van der Krol, A.R.   +7 more
openaire   +2 more sources

Permethylated N-Glycan Analysis with Mass Spectrometry

2013
Protein glycosylation plays an important role in multiple cell functions, and aberrations of protein glycosylation are associated with various malignancies including cancer. In this chapter, we provide a detailed protocol for MALDI MS analysis of permethylated N-glycans extracted from human serum proteins.
Zhenxin, Lin, David M, Lubman
openaire   +2 more sources

Cytokine Sensitivity and N‐Glycan Processing Mutations

2006
The EGF and TGF-beta families of cytokines are critical regulators of cell proliferation, morphogenesis, and tissue repair. The signaling pathways downstream of EGF and TGF-beta receptors also contribute to cancer growth and metastasis. Cytokine receptors are glycoproteins, and we have recently shown that GlcNAc-branching of N-glycans enhances their ...
Emily A, Partridge, Pam, Cheung, James W, Dennis   +2 more
openaire   +2 more sources

Chemical and enzymatic N-glycan release comparison for N-glycan profiling of monoclonal antibodies expressed in plants

Analytical Biochemistry, 2010
Plants synthesize N-glycans containing the antigenic sugars alpha(1,3)-fucose and beta(1,2)-xylose. Therefore it is important to monitor these N-glycans in monoclonal antibodies produced in plants (plantibodies). We evaluated several techniques to characterize the N-glycosylation of a plantibody produced in tobacco plants with and without the KDEL ...
Triguero, Ada, Cabrera, Gleysin, Royle, Louise, Harvey, David, Rudd, Pauline, Dwek, Raymond, Bardor, Muriel, Lerouge, Patrice, Cremata, José   +8 more
openaire   +3 more sources

Free Complex-Type N-Glycans

2014
The occurrence of free high-mannose type N-glycans is well demonstrated in mammalian cells. However, free complex-type N-glycans are not normally observed. Recent glycomic analyses of human cancers have revealed the accumulation of significant amounts of free sialylated complextypeN-glycans in pancreatic and prostate cancers.
openaire   +1 more source

N-Glycan biosynthesis

Reactome - a curated knowledgebase of biological pathways, 2010
G Dall'Olio, P Gagneux
openaire   +1 more source

N-Glycans and Diabetes

2023
Tamara Štambuk, Gordan Lauc
openaire   +1 more source

Glycan optimization of a human monoclonal antibody in the aquatic plant Lemna minor

Nature Biotechnology, 2006
K. Cox, J. Sterling, Jeff T. Regan, J. R. Gasdaska, K. Frantz, Charles G. Peele, A. Black, D. Passmore, Cristina Moldovan-Loomis, M. Srinivasan, Severino Cuison, P. Cardarelli, L. Dickey   +12 more
semanticscholar   +1 more source