Results 1 to 10 of about 70,171 (129)

Cryo-EM structure and regulation of human NAD kinase [PDF]

Science Advances
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is a crucial reducing cofactor for reductive biosynthesis and protection from oxidative stress. To fulfill their heightened anabolic and reductive power demands, cancer cells must boost their NADPH production.
Prakash P. Praharaj, Yang Li, Charline Mary, Mona H. Soflaee, Kevin Ryu, Dohun Kim, Diem H. Tran, Trishna Dey, Harrison J. Tom, Halie Rion, Muriel Gelin, Andrew Lemoff, Lauren G. Zacharias, João S. Patricio, Thomas P. Mathews, Zhe Chen, Corinne Lionne, Gerta Hoxhaj, Gilles Labesse   +18 more
europepmc   +4 more sources

Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay [PDF]

BIO-PROTOCOL, 2020
Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP. The NAD kinase responds to various environmental stimuli and its activity is regulated via various regulatory pathways, such as Ca2+-dependent and redox-dependent signals. Conventional in vitro NAD kinase
Yuuma Ishikawa, Maki Kawai-Yamada, Shin-nosuke Hashida   +2 more
openaire   +5 more sources

Mitochondrial NAD kinase in health and disease [PDF]

Redox Biology, 2023
Nicotinamide adenine dinucleotide phosphate (NADP), a co-enzyme and an electron carrier, plays crucial roles in numerous biological functions, including cellular metabolism and antioxidation. Because NADP is subcellular-membrane impermeable, eukaryotes compartmentalize NAD kinases (NADKs), the NADP biosynthetic enzymes.
Ren Zhang, Kezhong Zhang
openaire   +4 more sources

Molecular properties and regulation of NAD+ kinase (NADK) [PDF]

Redox Biology, 2023
Nicotinamide adenine dinucleotide (NAD+) kinase (NADK) phosphorylates NAD+, thereby producing nicotinamide adenine dinucleotide phosphate (NADP). Both NADK genes and the NADP(H)-producing mechanism are evolutionarily conserved among archaea, bacteria, plants and mammals.
Shin-ichi Oka, Allen Sam Titus, Daniela Zablocki, Junichi Sadoshima   +3 more
openaire   +4 more sources

New Chemical Probe Targeting Bacterial NAD Kinase [PDF]

Molecules, 2020
Nicotinamide adenine dinucleotide (NAD) kinases are essential and ubiquitous enzymes involved in the tight regulation of NAD/nicotinamide adenine dinucleotide phosphate (NADP) levels in many metabolic pathways. Consequently, they represent promising therapeutic targets in cancer and antibacterial treatments.
Clément, David, Leseigneur, Clarisse, Gelin, Muriel, Coelho, Dylan, Huteau, Valérie, Lionne, Corinne, Labesse, Gilles, Dussurget, Olivier, Pochet, Sylvie   +8 more
openaire   +6 more sources

The NAD Kinases of Saccharomyces cerevisiae [PDF]

European Journal of Biochemistry, 1970
Subcellular fractions of a strain of Saccharomyces cerevisiae have been prepared by enzymic digestion of the cell walls, osmotic lysis of the protoplasts and sorbitol density gradient centrifugation of the lysate. The mitochondrial fraction has both NAD‐ and NADH‐phosphorylating enzymes, whilst the cytoplasm contains a single NAD‐specific kinase. These
D.K. Apps
openaire   +5 more sources

Inhibition of pigeon liver NAD kinase by a non‐phosphorylatible analogue of NAD [PDF]

FEBS Letters, 1971
Nicotinamide-2’-deoxy-adenosine dinucleotide tNdAD) has previously been prepared enzymatically [ 11 and chemically [2] , and its interactions with dehydrogenases were studied. Since this analogue of NAD differs from the natural coenzyme in lacking the 2’-hydroxyl on the adenosine ribose, it cannot be phosphorylated by NAD kinase (ATP:NAD 2 ...
David K. Apps
openaire   +5 more sources

NAD Kinases Use Substrate-assisted Catalysis for Specific Recognition of NAD [PDF]

Journal of Biological Chemistry, 2007
Here we describe the crystal structures of the NAD kinase (LmNADK1) from Listeria monocytogenes in complex with its substrate NAD, its product NADP, or two synthesized NAD mimics. We identified one of the NAD mimics, di-adenosine diphosphate, as a new substrate for LmNADK1, whereas we showed that the closely related compound di-5'-thioadenosine is a ...
Gilles Labesse, Gilles Labesse, Sylvie Pochet, Sylvie Pochet, Liliane Assairi, Liliane Assairi, G. Poncet-Montange, G. Poncet-Montange, Stefan T. Arold, Stefan T. Arold   +9 more
openaire   +6 more sources

Metabolically distinct roles of NAD synthetase and NAD kinase define the essentiality of NAD and NADP in Mycobacterium tuberculosis [PDF]

mBio, 2023
ABSTRACT Nicotinamide adenine dinucleotide (NAD) and its phosphorylated derivative (NADP) are essential cofactors that participate in hundreds of biochemical reactions and have emerged as therapeutic targets in cancer, metabolic disorders, neurodegenerative diseases, and infections, including tuberculosis.
Ritu Sharma, Travis E. Hartman, Tiago Beites, Jee-Hyun Kim, Hyungjin Eoh, Curtis A. Engelhart, Linnan Zhu, Daniel J. Wilson, Courtney C. Aldrich, Sabine Ehrt, Kyu Young Rhee, Dirk Schnappinger   +11 more
openaire   +4 more sources

Kinetic Studies of Pigeon Liver NAD Kinase [PDF]

European Journal of Biochemistry, 1968
A procedure for the purification of NAD kinase from pigeon liver acetone powder is described. Although not homogeneous, the preparation is largely free of enzymes which interfere with the assay of NAD kinase. The kinetics with magnesium as the activating ion are described: from Interrelationships between the kinetic coefficients, the modes of ...
D.K. Apps
openaire   +5 more sources