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Measurement of Chloroplastic NAD Kinase Activity and Whole Tissue NAD Kinase Assay [PDF]

open access: yesBio-Protocol, 2020
Nicotinamide adenine dinucleotide phosphate (NADP) synthesis requires nicotinamide adenine dinucleotide (NAD) kinase activity, substrate NAD and ATP.
Yuma Ishikawa   +2 more
doaj   +6 more sources

Mitochondrial NAD kinase in health and disease [PDF]

open access: yesRedox Biology, 2023
Nicotinamide adenine dinucleotide phosphate (NADP), a co-enzyme and an electron carrier, plays crucial roles in numerous biological functions, including cellular metabolism and antioxidation.
Ren Zhang, Kezhong Zhang
doaj   +4 more sources

New Chemical Probe Targeting Bacterial NAD Kinase [PDF]

open access: yesMolecules, 2020
Nicotinamide adenine dinucleotide (NAD) kinases are essential and ubiquitous enzymes involved in the tight regulation of NAD/nicotinamide adenine dinucleotide phosphate (NADP) levels in many metabolic pathways.
David A. Clément   +8 more
doaj   +8 more sources

Metabolically distinct roles of NAD synthetase and NAD kinase define the essentiality of NAD and NADP in Mycobacterium tuberculosis [PDF]

open access: yesmBio, 2023
Nicotinamide adenine dinucleotide (NAD) and its phosphorylated derivative (NADP) are essential cofactors that participate in hundreds of biochemical reactions and have emerged as therapeutic targets in cancer, metabolic disorders, neurodegenerative ...
Ritu Sharma   +11 more
doaj   +4 more sources

Molecular properties and regulation of NAD+ kinase (NADK). [PDF]

open access: yesRedox Biol, 2023
Nicotinamide adenine dinucleotide (NAD+) kinase (NADK) phosphorylates NAD+, thereby producing nicotinamide adenine dinucleotide phosphate (NADP). Both NADK genes and the NADP(H)-producing mechanism are evolutionarily conserved among archaea, bacteria, plants and mammals.
Oka SI   +3 more
europepmc   +4 more sources

The NAD Kinases of Saccharomyces cerevisiae [PDF]

open access: bronzeEuropean Journal of Biochemistry, 1970
Subcellular fractions of a strain of Saccharomyces cerevisiae have been prepared by enzymic digestion of the cell walls, osmotic lysis of the protoplasts and sorbitol density gradient centrifugation of the lysate. The mitochondrial fraction has both NAD‐ and NADH‐phosphorylating enzymes, whilst the cytoplasm contains a single NAD‐specific kinase. These
D.K. Apps
openalex   +5 more sources

Inhibition of pigeon liver NAD kinase by a non‐phosphorylatible analogue of NAD [PDF]

open access: bronzeFEBS Letters, 1971
Nicotinamide-2’-deoxy-adenosine dinucleotide tNdAD) has previously been prepared enzymatically [ 11 and chemically [2] , and its interactions with dehydrogenases were studied. Since this analogue of NAD differs from the natural coenzyme in lacking the 2’-hydroxyl on the adenosine ribose, it cannot be phosphorylated by NAD kinase (ATP:NAD 2 ...
David K. Apps
openalex   +5 more sources

Kinetic Studies of Pigeon Liver NAD Kinase [PDF]

open access: bronzeEuropean Journal of Biochemistry, 1968
A procedure for the purification of NAD kinase from pigeon liver acetone powder is described. Although not homogeneous, the preparation is largely free of enzymes which interfere with the assay of NAD kinase. The kinetics with magnesium as the activating ion are described: from Interrelationships between the kinetic coefficients, the modes of ...
D.K. Apps
openalex   +5 more sources

Physiological Significance of NAD Kinases in Cyanobacteria

open access: yesFrontiers in Plant Science, 2019
Unicellular cyanobacteria are thought to be the evolutionary ancestors of plant chloroplasts and are widely used both for chemical production and as model organisms in studies of photosynthesis. Although most research focused on increasing reducing power
Yuuma Ishikawa, Maki Kawai-Yamada
doaj   +4 more sources

An increase of NAD+ kinase activity in Neurospora cells during adaptation to environmental stress [PDF]

open access: hybrid, 1984
An increase of NAD+ kinase activity in Neurospora cells during adaptation to environmental ...
T. P. Afanasieva   +2 more
openalex   +4 more sources

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