Results 141 to 150 of about 33,309 (204)
Animal Research and One Health, EarlyView.Dietary glutamine (Gln) supplementation increased the ATP levels and promoted cell proliferation in the rumen epithelium of feed‐restricted yaks. Besides, Gln inhibited ROS accumulation and reduced oxidative damage and apoptosis in the rumen epithelium of feed‐restricted yaks.
Ziqi Yue +10 more
wiley +1 more source
Luminescent Turn-On Upconversion Nanoprobes for Monitoring NADH In Vivo
Mitochondrial damage is implicated with a variety of pathological processes and is featured by the dramatically raised NADH level. To date, the detection and in situ imaging of NADH in living animals is still a challenge.
Liqiang Yang (692639) +6 more
core +1 more source
British Journal of Clinical Pharmacology, EarlyView.Abstract Aim Critical care for COVID‐19 patients presents unique challenges, particularly in sedation management. Patients demonstrate notable dose‐related resistance to propofol, potentially due to complex pharmacokinetic alterations. Emerging evidence suggests that plasma extracellular vesicle‐associated miRNA represents valuable predictive ...
Debora Curci +10 more
wiley +1 more source
Artificial Cell Catalysts for Biotransformation: Design Principles and Process Integration
Biotechnology and Bioengineering, EarlyView.ABSTRACT Artificial cell catalysts are emerging as programmable biocatalytic platforms for sustainable molecular conversion. By reconstituting enzymes and functional modules within synthetic compartments, they combine the selectivity of biological catalysis with the design flexibility of synthetic materials.
Iori Kobayashi +2 more
wiley +1 more source
Metabolic engineering of cofactors (NADH/NAD+) in Escherichia coli
, 2002 Metabolic engineering studies have generally focused on manipulating enzyme levels. However, cofactors play a major role in the production of fermentation products.
Berrios-Rivera, Susana Joanne
core
Biotechnology and Bioengineering, EarlyView.ABSTRACT The biocatalytic production of nicotinic acid using nitrilases has emerged as a promising alternative to conventional chemical synthesis, offering improved selectivity and environmental sustainability. In this study, we developed a continuous‐flow millireactor system employing Escherichia coli cells expressing a nitrilase from Paraburkholderia
Jonathan Bassut +8 more
wiley +1 more source
Biotechnology and Bioengineering, EarlyView.ABSTRACT A method to select Escherichia coli citrate synthase variants having reduced activity was developed based initially on the increased generation of acid, observed visually by prolonged color change in the pH indicator bromocresol purple. Five hundred fifty‐two potential variants were isolated and each were cultured on glucose as sole carbon ...
Jeffrey K. Dodelin +3 more
wiley +1 more source
, 2012 碩士NAD+/NADH是相當重要的輔酶，超過300種的酵素會利用此對分子以進行氧化還原作用，因此NADH的量測可發展大量的生化感測器。而在歷史文獻中，已經知道在石墨電極上偵測NADH的氧化時，需使用相當高的過電壓，且在NADH氧化過程中會產生自由基陽離子中間體的氧化物種吸附在電極表面上，造成電極產生毒化現象造成直傳的阻礙以及靈敏度下降。因此，本研究傾向發展低電位偵測NADH的方式。 本研究中進一步探討流注分析系統(FIA)以評估偵測NADH時的最佳化條件：70% Co3O4修飾電極，偵測環境為0.2M ...
陳映慈; Chen, Ying-Cih
core
Biotechnology and Bioengineering, EarlyView.ABSTRACT Inclusion body (IB) processes yielding the recombinant product only as IBs are long‐established in the biopharmaceutical industry. Yet, for recombinant proteins that accumulate both IBs and soluble fraction depending on the selected upstream processing (USP) strategy, the influence of process parameters on the partitioning between both forms ...
Mohamed Elshazly +7 more
wiley +1 more source
Harnessing ferroptosis from multilayer defense networks to nanoplatforms for specific cancer therapy
BMEMat, EarlyView.Nanomaterials target metabolically‐regulated ferroptosis for cancer therapy. Iron‐based or alternative nanoplatforms integrate ferroptosis with chemotherapy, immunotherapy, or radiotherapy. They enable stimulus‐responsive therapies (photothermal, photodynamic, sonodynamic) activated by near‐infrared, light, or ultrasound, achieving potent synergistic ...
Xinyue Xu +5 more
wiley +1 more source