A Fast and Accurate Method to Identify and Quantify Enzymes in Brush-Border Membranes: In Situ Hydrolysis Followed by Nano LC-MS/MS [PDF]
Methods and Protocols, 2020 A simple method for the identification of brush-border membrane α-glucosidases is described. The proteins were first solubilized and separated in a gel under native, non-denaturing, conditions.
Antonio Brun +5 more
doaj +2 more sources
Quantifying the Role of Lysine in Prion Replication by Nano-LC Mass Spectrometry and Bioassay. [PDF]
Front Bioeng Biotechnol, 2020 Prions propagate by a template driven process, inducing the normal cellular isoform (PrPC) to adopt the prion (PrPSc) conformation. In PrPC, the positions of lysines are highly conserved and strongly influence prion propagation. In this study, covalent modification was used to quantitate the role of lysines in the PrPSc template that drives prion ...
Silva CJ, Erickson-Beltran ML, Dynin IC.
europepmc +5 more sources
Combination of GC-IMS and Nano-LC/HRMS Reveals the Mechanism of Superheated Steam Glycosylation Modification in Improving Oyster Peptide Flavor [PDF]
FoodsThis study investigated the effect of superheated steam (SS) assisted glycosylation modification on the flavor profile of oyster peptides (OP), and explored the correlation between key flavor compounds and glycosylation degree using Gas Chromatography ...
Li-Hong Wang +5 more
doaj +2 more sources
Nano-LC with New Hydrophobic Monolith Based on 9-Antracenylmethyl Methacrylate for Biomolecule Separation. [PDF]
Int J Mol SciIn this study, new monolithic poly(9-anthracenylmethyl methacrylate-co-trimethylolpropane trimethacrylate (TRIM) columns, referred as ANM monoliths were prepared, for the first time, and were used for the separation media for biomolecules and proteomics analysis by nano-liquid chromatography (nano-LC).
Aydoğan C, Alharthi S.
europepmc +3 more sources
Simultaneous Analysis of L-Carnitine and Acetyl-L-Carnitine in Food Samples by Hydrophilic Interaction Nano-Liquid Chromatography [PDF]
Methods and ProtocolsL-Carnitine (L-CAR) and acetyl-L-carnitine (Acetyl L-CAR) are the essential cofactor compounds in lipid metabolism and are used in the treatment of various diseases.
Cemil Aydoğan +2 more
doaj +2 more sources
Enantiomeric Separation of Colchicine and Lacosamide by Nano-LC. Quantitative Analysis in Pharmaceutical Formulations [PDF]
Separations, 2020 A chiral analytical methodology was developed by nano-liquid chromatography (nano-LC) enabling the enantiomeric separation of two chiral drugs, lacosamide (novel antiepileptic drug) and colchicine (antiuremic drug), commercialized as pure enantiomers.
Natalia Casado +3 more
doaj +3 more sources
Label-Free Quantitative Proteomics Analysis of COVID-19 Vaccines by Nano LC-HRMS. [PDF]
Vaccines (Basel)A nanoliter liquid chromatography–high resolution mass spectrometry-based method was developed for quantitative proteomics analysis of COVID-19 vaccines. It can be used for simultaneous qualitative and quantitative analysis of target proteins and host cell proteins (HCPs) in vaccine samples. This approach can directly provide protein information at the
Zhao H +6 more
europepmc +4 more sources
Miniaturized-LC in the Analysis of Emerging Organic Contaminants in Food and Environmental Samples: Recent Advances and Applications [PDF]
MoleculesMini-LC systems, including Cap-LC, Nano-LC and Chip-LC, offer a sustainable alternative to conventional LC methods thanks to their reduced solvent consumption, enhanced separation efficiency and environmentally friendly operation. Integrating micro-scale
Cemil Aydoğan +4 more
doaj +2 more sources
Nano-LC with new neutral monolith for biomolecule separation
Green Analytical ChemistryA new capillary monolith based on the co-polymerization of 2-napthylmethacrylate (NMM) as the functional monomer and trimethylolpropane trimethacrylate as the crosslinker was introduced for nano-LC.
Cemil Aydoğan +3 more
doaj +2 more sources
Micro and Nano Engineering, 2021 This study demonstrates the fabrication of periodically unidirectional nano-wedges using nanoimprint lithography to achieve homeotropic alignments in sandwiched liquid crystal (LC) cells.
Chia-Rong Sheu +2 more
doaj +1 more source