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Application of ultrasound-assisted physical mixing treatment improves in vitro protein digestibility of rapeseed napin

open access: yesUltrasonics Sonochemistry, 2020
The in vitro protein digestibility (IVPD) of napin was studied using different pretreatment methods, including ultrasound, mixing napin with lactalbumin, and ultrasound-assisted protein mixing. The relationships between IVPD, molecular structure, and disulfide bonds were explored, showing that the IVPD of napin was the highest compared with the control
Feiran Xu, Xiang Xiao, Na Zhang
exaly   +3 more sources
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In vitro refolded napin-like protein of Momordica charantia expressed in Escherichia coli displays properties of native napin

Biochimica Et Biophysica Acta - Proteins and Proteomics, 2006
Napins belong to the family of 2S albumin seed storage proteins and are shown to possess antifungal activity. Napins, in general, consist of two subunits (derived from single precursor) linked by disulphide bridges. Usually, reducing environment of the E.
Tejram Sahu, Aparna Dixit
exaly   +3 more sources

An integrated method of isolating napin and cruciferin from defatted canola meal

open access: yesLWT - Food Science and Technology, 2015
Abstract Canola proteins are known to have great potential for use in food and non-food applications due to their nutritional, biological and functional properties. However, canola protein extraction remains a challenge, due mainly to the complexity of canola proteins and the presence a variety of undesirable compounds.
Jianping Wu
exaly   +2 more sources

A strategy for targeting recombinant proteins to protein storage vacuoles by fusion to Brassica napus napin in napin-depleted seeds

Protein Expression and Purification, 2014
Seeds are capable of accumulating high levels of seed storage proteins (SSP), as well as heterologous proteins under certain conditions. Arabidopsis thaliana was used to develop a strategy to deplete seeds of an endogenous SSP and then replenish them with the same protein fused to a heterologous protein.
Dwayne D Hegedus   +2 more
exaly   +3 more sources

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