Results 161 to 170 of about 3,644 (182)

Proteomic identification of allergenic seed proteins, napin and cruciferin, from cold-pressed rapeseed oils

Food Chemistry, 2015
In Finland and France atopic children commonly react to seeds of oilseed rape and turnip rape in skin prick tests (SPT) and open food challenges. These seeds are not as such in dietary use and therefore the routes of sensitization are unknown. Possible allergens were extracted from commercial cold-pressed and refined rapeseed oils and identified by gel-
Puumalainen, T. J., Puustinen, A., Poikonen, S., Turjanmaa, K., Palosuo, T., Vaali, K.   +5 more
openaire   +3 more sources

The Biosynthesis, Structure and Properties of Napin - the Storage Protein From Rape Seeds

Journal of Plant Physiology, 1999
Summary A growing interest in the study of many aspects of seed storage proteins has been observed recently. Various functional and structural data on napin, the 2S storage protein of rape ( Brassica napus ), have been accumulated. The primary structures of genes, eDNA as well as amino acid sequences of napin have been solved. There are also NMR data
Jan Barciszewski
exaly   +2 more sources

Isolation and characterization of an expressed napin gene fromBrassica rapa

Seed Science Research, 1991
AbstractAn expressed napin storage protein gene fromBrassica rapa, BcNA1, has been cloned and sequenced. The gene is a member of a family of four to seven napin genes inB. rapaand is highly expressed in developing seeds. An expression cassette containing the DNA flanking the napin coding region of BcNA1 has been engineered and demonstrated to function ...
Jean C. Kridl, David W. McCarter, Ronald E. Rose, Donna E. Scherer, Deborah S. Knutzon, Sharon E. Radke, Vic C. Knauf   +6 more
openaire   +1 more source

Expression of the BnmNAP subfamily of napin genes coincides with the induction of Brassica microspore embryogenesis

Plant Molecular Biology, 1994
Brassica napus cv. Topas microspores can be diverted from pollen development toward haploid embryo formation in culture by subjecting them to a heat stress treatment. We show that this switch in developmental pathways is accompanied by the induction of high levels of napin seed storage protein gene expression.
K A, Boutilier, M J, Ginés, J M, DeMoor, B, Huang, C L, Baszczynski, V N, Iyer, B L, Miki   +6 more
exaly   +3 more sources

pH and NaCl dependent complexation between rapeseed cruciferin and napin

Food Hydrocolloids
The interaction between the main protein components of rapeseed, also called canola, was investigated in water as a function of the pH and in the presence of NaCl. Firstly, the solubility of purified cruciferin and napin was determined and the soluble fraction was characterized using light scattering techniques.
Colleen P.K. Mudau, Maria Moutkane, Christophe Chassenieux, Marianne N. Lund, Taco Nicolai   +4 more
openaire   +2 more sources

The structure and properties of Napin-seed storage protein from rape (Brassica napus L.)

Nahrung / Food, 1998
Purification to homogeneity of 2S albumin storage protein from rape seeds (napin) was achieved and its secondary structure and conformational stability were characterised by circular dichroism (CD), and high-sensitivity differential scanning calorimetry (HS-DSC).
Krzyaniak, A., Burova, T.V., Haertlé, Thomas, Barciszewski, J.   +3 more
openaire   +2 more sources

A 13-week dietary toxicity study in rats of a Napin-Rich Canola Protein Isolate

Regulatory Toxicology and Pharmacology, 2009
The objective was to study the safety of a Napin-Rich Canola Protein Isolate (NRCPI) fed to rats at various levels for 13-weeks. The study included four groups (20 animals/sex/group) of young Sprague Dawley rats. They were fed ad libitum with an AIN-93G based protein-free diet containing, respectively, 5%, 10% and 20% (w/w) NRCPI (test article) or 20% (
Luis A, Mejia, Chandrashekhar K, Korgaonkar, Martin, Schweizer, Christopher, Chengelis, Meliton, Novilla, Ellen, Ziemer, Patricia S, Williamson-Hughes, Richard, Grabiel, Mark, Empie   +8 more
openaire   +2 more sources

Selective extraction, structural characterisation and antifungal activity assessment of napins from an industrial rapeseed meal

Food Chemistry, 2012
This article reports an extraction-purification of napins from an industrial rapeseed meal and the assessment of their antimicrobial activity against Fusarium langsethiae. The best extraction conditions are observed at pH 2, 12% (w/w) of rapeseed meal after 15 min of extraction in water at room temperature.
Nioi, Claudia, Kapel, Romain, Rondags, Emmanuel, Marc, Ivan   +3 more
openaire   +2 more sources

Isolation and sequence analysis of napin seed specific promoter from Iranian Rapeseed (Brassica napus L.)

Gene, 2015
Rapeseed (Brassica napus L.) has become an important crop during the last 30years. In addition to a high lipid level, the seeds also have a significant protein content, which constitutes 20-25% of the dry seed weight. The synthesis of storage proteins is primarily controlled at transcriptional level and seed-specific expression has been shown to be ...
Maryam, Sohrabi, Alireza, Zebarjadi, Abdollah, Najaphy, Danial, Kahrizi   +3 more
openaire   +2 more sources

Differential expression of members of the napin storage protein gene family during embryogenesis inBrassica napus

Plant Molecular Biology, 1991
S1 nuclease analysis and sub-family-specific oligonucleotide probes were used to characterize the expression during embryogenesis of the napin storage protein gene family of Brassica napus (oilseed rape). The expression of one sub-class represented by the napin gene gNa peaks and declines earlier than the other members of the family. This sub-class was
K S, Blundy, M A, Blundy, M L, Crouch
openaire   +2 more sources