Results 131 to 140 of about 22,312,818 (260)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Implementing organisational change using action research in two Australian organisations

, 2017
This paper is based on two organisational change projects implemented by the authors in their respective organisations. Both projects used an action research intervention to bring about a market orientation in the organisations and also contributed to ...
Cook, Andrew, Kalendra, Diane Robyn
core  

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Action learning, action research: Towards greater collaboration amongst networks

, 2018
Recent conversations with a variety of action learning and action research practitioners around the world have identified a growing desire for collaboration between networks.
Bradley, Colin
core  

Operational research leading to state-level policy change in tuberculosis undernutrition in Chhattisgarh, India. [PDF]

Public Health Action
Malik C, Gupta V, Shringarpure K, Gupte HA, Keshri VR, Damani M, Shah NN, Sonwani K, Nanda P, Sharma A, Mattoo SK, Shewade HD.   +11 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Feasibility of tuberculosis drug-induced liver injury screening under programme settings in Southern India. [PDF]

Public Health Action
Natarajan M, Verma M, Akshaya KM, Manohar G, Rathinam V, Ramalingam S, Kaleeswari M, Ganesan K, Gouroumourty R, Vasudevan D, Anandakrishnan S, Ramachandran A, Ramesh PM, Frederick A.   +13 more
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Implementing school-based nutrition interventions in low- and middle-income countries: a scoping review of implementation strategies, frameworks, and outcomes. [PDF]

Glob Health Action
Kamyshnyi O, Halabitska I, Petakh P, Bhattarai S, Sen A.   +4 more
europepmc   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source