Results 141 to 150 of about 1,618,479 (311)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

From SO2 to Greenhouse Gases: Trends and Events Shaping Future Emissions Trading Programs in the United States [PDF]

Cap-and-trade programs have become widely accepted for the control of conventional air pollution in the United States. However, there is still no political consensus to use these programs to address greenhouse gases. Meanwhile, in the wake of the success
Kruger, Joseph
core  

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Soil legacy data rescue via GlobalSoilMap and other international and national initiatives. [PDF]

GeoResJ, 2017
Arrouays D, Leenaars JGB, Richer-de-Forges AC, Adhikari K, Ballabio C, Greve M, Grundy M, Guerrero E, Hempel J, Hengl T, Heuvelink G, Batjes N, Carvalho E, Hartemink A, Hewitt A, Hong SY, Krasilnikov P, Lagacherie P, Lelyk G, Libohova Z, Lilly A, McBratney A, McKenzie N, Vasquez GM, Leatitia Mulder V, Minasny B, Luca M, Odeh I, Padarian J, Poggio L, Roudier P, Saby N, Savin I, Searle R, Solbovoy V, Thompson J, Smith S, Sulaeman Y, Vintila R, Rossel RV, Wilson P, Zhang GL, Swerts M, Oorts K, Karklins A, Feng L, Ibelles Navarro AR, Levin A, Laktionova T, Dell'Acqua M, Suvannang N, Ruam W, Prasad J, Patil N, Husnjak S, Pasztor L, Okx J, Hallet S, Keay C, Farewell T, Lilja H, Juilleret J, Marx S, Takata Y, Kazuyuki Y, Mansuy N, Panagos P, Van Liedekerke M, Skalsky R, Sobocka J, Kobza J, Eftekhari K, Kacem Alavipanah S, Moussadek R, Badraoui M, Da Silva M, Paterson G, da Conceicao Gonsalves M, Theocharopoulos S, Yemefack M, Tedou S, Vrscaj B, Grob U, Kozak J, Boruvka L, Dobos E, Taboada M, Moretti L, Rodriguez D.   +88 more
europepmc   +1 more source

Digital twins to accelerate target identification and drug development for immune‐mediated disorders

FEBS Open Bio, EarlyView.
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

National initiatives to improve outcomes from out-of-hospital cardiac arrest in England. [PDF]

Emerg Med J, 2016
Perkins GD, Lockey AS, de Belder MA, Moore F, Weissberg P, Gray H, Community Resuscitation Group.   +6 more
europepmc   +1 more source

Horizon scanning: bringing strategic insight to national security policymaking [PDF]

The Australian Government has recently undertaken a number of concrete initiatives to enhance national security policy coordination. As of the date of publication of this paper, some have been implemented, such as the ‘All Hazards National Assessment’ on
David Connery
core  

Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment

Aging and Cancer, EarlyView.
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey, Murilo Ramos Rocha, Cheyenne Palm, Vania Wang, Denys Balandin, Ethan Black, Kevin Y. Zhang, Fan Huang, Daniel J. Zabransky, Yash Chhabra, Mareike Peters, Gabriel Ghiaur, Ashani Weeraratna   +12 more
wiley   +1 more source