Results 121 to 130 of about 792 (145)

Microbial nattokinase: from synthesis to potential application

Food & Function, 2023
Outline of the source, purification, synthesis process, physical and chemical properties, intervention to disease and improvement of activity of nattokinase.
Yanan Sheng, Jiani Yang, Changyuan Wang, Xindi Sun, Lei Yan   +4 more
openaire   +2 more sources

Development of Novel Monoclonal Antibodies Against Nattokinase

Monoclonal Antibodies in Immunodiagnosis & Immunotherapy, 2023
Nattokinase is a protease produced by Bacillus subtilis var. natto that exhibits various beneficial biological effects. Thus, a reliable assay to determine nattokinase levels is needed. In this study, we developed novel mouse monoclonal antibodies (mAbs)
Takashi Tanikawa, James Yu, Kate Hsu, Shinder Chen, Ayako Ishii, Takami Yokogawa, Yutaka Inoue, Masashi Kitamura   +7 more
openaire   +2 more sources

Nattokinase atherothrombotic prevention study: A randomized controlled trial

Clinical Hemorheology and Microcirculation, 2021
BACKGROUND: Described to be antithrombotic and antihypertensive, nattokinase is consumed for putative cardiovascular benefit. However, no large-scale, long-term cardiovascular study has been conducted with nattokinase supplementation. OBJECTIVE: To determine the effect of nattokinase on subclinical atherosclerosis progression and atherothrombotic ...
Howard N, Hodis, Wendy J, Mack, Herbert J, Meiselman, Vijay, Kalra, Howard, Liebman, Juliana, Hwang-Levine, Laurie, Dustin, Naoko, Kono, Melissa, Mert, Rosalinda B, Wenby, Emiliano, Huesca, Leanne, Rochanda, Yanjie, Li, Mingzhu, Yan, Jan A, St John, Lora, Whitfield   +15 more
openaire   +2 more sources

Nattokinase: production and application

Applied Microbiology and Biotechnology, 2014
Nattokinase (NK, also known as subtilisin NAT) (EC 3.4.21.62) is one of the most considerable extracellular enzymes produced by Bacillus subtilis natto. The main interest about this enzyme is due to its direct fibrinolytic activity. Being stable enough in the gastrointestinal tract makes this enzyme a useful agent for the oral thrombolytic therapy ...
Fatemeh, Dabbagh, Manica, Negahdaripour, Aydin, Berenjian, Abdolazim, Behfar, Fatemeh, Mohammadi, Mozhdeh, Zamani, Cambyz, Irajie, Younes, Ghasemi   +7 more
openaire   +2 more sources

Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM

Protein Expression and Purification, 2023
Nattokinase, a serine protease, was discovered in Bacillus subtilis during the fermentation of a soybean byproduct. Nattokinase is essential for the lysis of blood clots and the treatment of cardiac diseases including atherosclerosis, thrombosis, high blood pressure, and stroke.
Akhilesh Modi, Ishan Raval, Pooja Doshi, Madhvi Joshi, Chaitanya Joshi, Amrutlal K. Patel   +5 more
openaire   +2 more sources

Nattokinase for prevention of thrombosis

American Journal of Health-System Pharmacy, 2006
Natto, a traditional food made of soybeans fermented with Bacillus subtilis , has been consumed for over 1000 years, especially in the Eastern Kanto region of Japan.[1][1]–[6][2] It has a cheese-like appearance, a strong smell, and a sticky consistency, which may affect its acceptance as a ...
Ming-Wai, Tai, Burgunda V, Sweet
openaire   +2 more sources

Mechanisms of Nattokinase in protection of cerebral ischemia

European Journal of Pharmacology, 2014
In vivo, the level of cyclic Adenosine Monophosphate (cAMP) and the pathway of the Janus Kinase1/Signal Transducers and Activators of Transcription1 (JAK1/STAT1) were studied. In vitro, the Ca(2+) mobilization in human platelet stimulated by thrombin was observed.
Hongrui, Ji, Liang, Yu, Keyu, Liu, Zhigang, Yu, Qian, Zhang, Fengjuan, Zou, Bo, Liu   +6 more
openaire   +2 more sources

Nattokinase: An Updated Critical Review on Challenges and Perspectives

Cardiovascular & Hematological Agents in Medicinal Chemistry, 2018
Natto, a fermented soybean food, has been consumed by oriental people for more than 1000 years. Nattokinase, formerly called subtilisin NAT is a well studied protease of microbial origin that possesses fibrinolytic (anti-clotting) activities. Due to its strong fibrinolytic and thrombolytic activity, Nattokinase is regarded as a precious dietary ...
Ethiraj, Selvarajan, Niharika, Bhatnagar
openaire   +2 more sources

Stabilization and Target Delivery of Nattokinase Using Compression Coating

Drug Development and Industrial Pharmacy, 2007
The aim of the work is to develop a new formulation in order to stabilize a nutraceutical enzyme Nattokinase (NKCP) in powders and to control its release rate when it passes through the gastrointestinal tract of human. NKCP powders were first compacted into a tablet, which was then coated with a mixture of an enteric material Eudragit L100-55 (EL100-55)
D, Law, Z, Zhang
openaire   +2 more sources

Secretory expression of a heterologous nattokinase in Lactococcus lactis

Applied Microbiology and Biotechnology, 2007
Nattokinase has been reported as an oral health product for the prevention of atherosclerosis. We developed a novel strategy to express a nattokinase from Bacillus subtilis in a live delivery vehicle, Lactococcus lactis. Promoter P( nisZ) and signal peptide SP(Usp) were used for inducible and secretory expression of nattokinase in L. lactis.
Xiaobo, Liang, Lixin, Zhang, Jin, Zhong, Liandong, Huan   +3 more
openaire   +2 more sources