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Purification, immobilization, and characterization of nattokinase on PHB nanoparticles
Bioresource Technology, 2009In this study, nattokinase was purified from Bacillus subtilis using ion exchange chromatography and immobilized upon polyhydroxybutyrate (PHB) nanoparticles. A novel strain isolated from industrial dairy waste was found to synthesize polyhydroxyalkanoates (PHA) and the strain was identified as Brevibacterium casei SRKP2.
Venkataraman, Deepak +3 more
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Microbial production of nattokinase: current progress, challenge and prospect
World Journal of Microbiology and Biotechnology, 2017Nattokinase (EC 3.4.21.62) is a profibrinolytic serine protease with a potent fibrin-degrading activity, and it has been produced by many host strains. Compared to other fibrinolytic enzymes (urokinase, t-PA and streprokinase), nattokinase shows the advantages of having no side effects, low cost and long life-time, and it has the potential to be used ...
Dongbo, Cai +2 more
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Bioproperties of Potent Nattokinase from Bacillus subtilis YJ1
Journal of Agricultural and Food Chemistry, 2010Fibrinolytic enzyme activity was observed during cultivation of Bacillus subtilis YJ1 in a medium containing 1% skim milk, 1% rice husk, 0.5% NaCl, and 0.25% glucose. It was purified to electrophoretical homogeneity after CM-sepharose FF chromatography. The specific activity and yield were 1791.9 FU/mg and 9.5%, respectively. This purified fibrinolytic
Li-Jung, Yin +2 more
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Journal of Biomedical Nanotechnology, 2015
The present research work describes the downstreaming of nattokinase (NK) produced by Bacillus subtilis under solid state fermentation; and the role of efficient oral formulation of purified NK in the management of thrombotic disorders. Molecular weight of purified NK was estimated to be 28 kDa with specific activity of 504.4 FU/mg.
Rohit, Kapoor +4 more
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The present research work describes the downstreaming of nattokinase (NK) produced by Bacillus subtilis under solid state fermentation; and the role of efficient oral formulation of purified NK in the management of thrombotic disorders. Molecular weight of purified NK was estimated to be 28 kDa with specific activity of 504.4 FU/mg.
Rohit, Kapoor +4 more
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Reverse micelles extraction of nattokinase from fermentation broth
Biochemical Engineering Journal, 2004Nattokinase is a new fibrinolytic enzyme, which cleaves directly cross-linked fibrin in vitro. As its name implies, it is produced in the natto fermentation process. In this study, the purification of nattokinase from fermentation broth with AOT/isooctane micellar solution as the extractant was studied.
Jun-Guo Liu +4 more
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Nattokinase: Structure, applications and sources
Biocatalysis and Agricultural Biotechnology, 2023Navid Jamali +7 more
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International Journal of Biological Macromolecules
Nattokinase (NK, EC 3.4.21.62) is an alkaline serine protease secreted by B. subtilis, which has a strong fibrinolytic activity in vitro and in vivo. Here, we fermented NK using a B. subtilis strain and purified the protein, designed a peptide segment derived from fibrin as a substrate of NK.
Jinwen, Li, Baohui, Li
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Nattokinase (NK, EC 3.4.21.62) is an alkaline serine protease secreted by B. subtilis, which has a strong fibrinolytic activity in vitro and in vivo. Here, we fermented NK using a B. subtilis strain and purified the protein, designed a peptide segment derived from fibrin as a substrate of NK.
Jinwen, Li, Baohui, Li
openaire +2 more sources