Results 71 to 80 of about 1,511,709 (299)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Bye-bye dark sky: is light pollution costing us more than just the night-time?

open access: yes, 2018
Humans, birds and several other animals are finding it increasingly challenging to experience night-time uninterrupted by artificial light, while some creatures are handling the change better than others.
West, S, Van Grouw, H, Lotzof, K
core  

Modeling Natural Light Availability in Skyscraper Farms

open access: yes, 2021
Lighting is a major component of energy consumption in controlled environment agriculture (CEA) operations. Skyscraper farms (multilevel production in buildings with transparent glazing) have been proposed as alternatives to greenhouse or plant factories
Timothy Shelford   +3 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Light attenuation characteristics of glacially-fed lakes

open access: yes, 2014
Transparency is a fundamental characteristic of aquatic ecosystems and is highly responsive to changes in climate and land use. The transparency of glacially-fed lakes may be a particularly sensitive sentinel characteristic of these changes.
Olson, MH   +8 more
core   +1 more source

Converting a Natural-Light-Driven Outward Proton Pump Rhodopsin into an Artificial Inward Proton Pump

open access: yes, 2023
Microbial rhodopsins are a large family of photoreceptive membrane proteins with diverse light-regulated functions. While the most ubiquitous microbial rhodopsins are light-driven outward proton (H+) pumps, new subfamilies of microbial rhodopsins ...
Hiromu Yawo   +7 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Diferentes substratos e ambientes no enraizamento in vitro de mirtilo Different substrates and environment conditions on in vitro rooting of blueberry

open access: yesCiência Rural, 2009
O objetivo neste trabalho foi comparar a capacidade de enraizamento in vitro de mirtilo (Vaccinium ashei Reade cv. 'Delite' e V. corymbosum L. cv. 'Georgiagem') e estudar o efeito de substratos alternativos ao ágar (perlita, vermiculita e ágar + carvão ...
Cláudia Roberta Damiani   +1 more
doaj   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

open access: yesFEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Absorbance based light emitting diode optical sensors and sensing devices [PDF]

open access: yes, 2008
The ever increasing demand for in situ monitoring of health, environment and security has created a need for reliable, miniaturised sensing devices. To achieve this, appropriate analytical devices are required that possess operating characteristics of ...
O'Toole, Martina   +5 more
core   +1 more source

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