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CRISPR-Cas9 enabled molecular genetic studies of natural rubber biosynthesis in lettuce

2022
Natural rubber (NR) is an industrially essential biopolymer due to its irreplicable properties. To date the only necessary components identified for NR biosynthesis are two membrane-bound proteins, cis-prenyltransferase (CPT) and CPT binding protein (CBP) that form a complex in which CPT catalyzes the addition of isopentyl pyrophosphate (IPP) subunits ...
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Type-B authentic response regulators regulate natural rubber biosynthesis by targeting small rubber particle proteins in rubber-producing plants

Functional & Integrative Genomics
Natural rubber, predominantly derived from Hevea brasiliensis, is an indispensable industrial material whose supply faces increasing threat. Alternative rubber-producing species, including Eucommia ulmoides and Taraxacum kok-saghyz, provide promising supplementary sources.
Xiaoyou, Wu +7 more
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UNRAVELING THE MYSTERY OF NATURAL RUBBER BIOSYNTHESIS. PART I: INVESTIGATION OF THE COMPOSITION AND GROWTH OF IN VITRO NATURAL RUBBER USING HIGH RESOLUTION SIZE EXCLUSION CHROMATOGRAPHY

Rubber Chemistry and Technology, 2011
Abstract Monitoring the growth of in vitro natural rubber was accomplished by high resolution size exclusion chromatography, SEC. Washed rubber particles isolated from H. brasiliensis latex, containing the rubber transferase enzyme, were used to catalyze the polymerization of synthetic isopentenyl pyrophosphate monomer in the presence of ...
Chengching K. Chiang +3 more
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Overexpression of the EuFPS5 gene in Hevea brasiliensis fails to produce Eucommia rubber but affects the biosynthesis of natural rubber