Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The state of natural capital: protecting and improving natural capital for prosperity and wellbeing [PDF]
, 2019 This is the final version. Available from Defra via the link in this record.This is the Natural Capital Committee’s (NCC) third report to government. The report sets out recommendations to achieve government’s vision, ‘to be the first generation to leave
Natural Capital Committee
core
Resilience After Fire in Mediterranean Reforested Areas
LandWildfires play a key ecological role in Mediterranean ecosystems but increasing fire frequency and severity, exacerbated by climate change, threaten vegetation integrity, biodiversity, and ecosystem services.
Ivo Rossetti +2 more
doaj +1 more source
EFFECTIVENESS OF AGRICULTURAL DEVELOPMENT PROGRAMME COMMUNICATION METHODS ON BROILER PRODUCTION TECHNOLOGY ADOPTION AMONG FARMERS IN ANAMBRA STATE, NIGERIA [PDF]
Scientific Papers Series : Management, Economic Engineering in Agriculture and Rural Development, 2019 Pines stands from outside their areal were created from 1972 in field and plain areas with the purpose of substituting degraded stands (oaks) or derived ones (linden, hornbeam).
Eucharia Ogochukwu CHUKWUDUM
doaj
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Trees, Forests and PeopleThe mechanisms by which environmental conditions in plantations and mixed natural forests drive changes in understory diversity through functional traits remain unclear, particularly regarding their dynamic effects during stand development.
Xiaoyan Zhang +5 more
doaj +1 more source
Irrigated urban trees exhibit greater functional trait plasticity compared to natural stands. [PDF]
Biol Lett, 2023 Ibsen PC +4 more
europepmc +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
The government’s response to the Natural Capital Committee’s third State of Natural Capital report [PDF]
, 2019 This is the final version. Available from Defra via the link in this recordThis response sets out how we will act on the recommendations in the Natural Capital Committee’s third State of Natural Capital report.
Natural Capital Committee
core
Forest Systems, 2016 Aim of study: a) To present a new version of the forest simulator Vorest, an individual-based spatially explicit model that uses weighted Voronoi diagrams to simulate the natural dynamics of forest stands with closed canopies.
Begoña Abellanas +4 more
doaj +1 more source