Results 171 to 180 of about 271,470 (302)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Effect of PBAT-g-MAH Compatibilization and Bamboo Flour Loadings on Melt Flow and Early Soil-Compost Mineralization of PLA Biocomposites for FFF 3D Printing. [PDF]

Polymers (Basel)
Paltán CA, Fajardo JI, Rodriguez DV, Carrasquero E.   +3 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Multiplexed Nanoparticle Protein Corona Enables Accurate and Precise Deep Plasma Proteomics. [PDF]

J Proteome Res
Huang T, Wang J, Stukalov A, Kreimer S, Zhao X, Cantrell LS, Zhou X, Brewer A, Ho G, Murolo F, Quach K, Figa M, Just S, Castro G, Elgierari E, Benz RW, Motamedchaboki K, Batzoglou S, Farokhzad OC, Van Eyk JE, Siddiqui A.   +20 more
europepmc   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

FEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu, Hao Jiang, Limin Chen, Fulai Zhou, Ying Jin, Mark L. Chiu   +5 more
wiley   +1 more source

Carbene-Metal-Amide Materials Design: Tailoring π-Extended Amides for High-Performance Organic Light-Emitting Diodes. [PDF]

Adv Sci (Weinh)
Brannan AC, Ji J, Phuoc NL, Lee D, Kim J, Linnolahti M, Yoo S, Romanov AS.   +7 more
europepmc   +1 more source

Evaluation of in vitro toxicity of common phytochemicals included in weight loss supplements using 1H NMR spectroscopy

FEBS Open Bio, EarlyView.
We investigated the toxicity of 12 active compounds commonly found in herbal weight loss supplements (WLS) using human liver and colon cell models. Epigallocatechin‐3‐gallate was the only compound showing significant toxicity. Metabolic profiling revealed protein degradation, disrupted energy and lipid metabolism suggesting that the inclusion of EGCG ...
Emily C. Davies, Garth L. Maker, Ian F. Musgrave, Samantha Lodge   +3 more
wiley   +1 more source

Design and Physicochemical Characterization of Hybrid PLGA-Curcumin/Carbon Dot Nanoparticles for Sustained Galantamine Release: A Proof-of-Concept Study. [PDF]

Biomolecules
Samiotaki C, Nanaki S, Bikiaris RE, Christodoulou E, Kyzas GZ, Barmpalexis P, Bikiaris DN.   +6 more
europepmc   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Highly Asymmetric Water Permeation in Dense Laminated Membranes. [PDF]

ACS Appl Polym Mater
Grillo L, Weder C.
europepmc   +1 more source