Results 91 to 100 of about 8,630,188 (308)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Radiofrequency and 2.45 GHz electron cyclotron resonance H− volume production ion sources

New Journal of Physics, 2016
The volume production of negative hydrogen ions ( ${{\rm{H}}}^{-}$ ) in plasma ion sources is based on dissociative electron attachment (DEA) to rovibrationally excited hydrogen molecules (H _2 ), which is a two-step process requiring both, hot electrons
O Tarvainen, S X Peng
doaj   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Beam formation in CERNs cesiated surfaces and volume H− ion sources

New Journal of Physics, 2016
At CERN, a high performance negative ion (NI) source is required for the 160 MeV H ^− linear accelerator named Linac4. The source should deliver 80 mA H ^− ion beams within an emittance of 0.25 mm·mrad.
Serhiy Mochalskyy, Jacques Lettry, Tiberiu Minea   +2 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Discharge characterization of two-region arc plasma (TRAP) ion source

Nuclear Engineering and Technology
The Korea Atomic Energy Research Institute (KAERI) is developing a novel Two-Region Arc Plasma Ion Source (TRAP) as a negative hydrogen (deuterium) ion source for a Neutral Beam Injection (NBI) system in a fusion tokamak.
Kihyun Lee, Seung Ho Jeong, Tae-Seong Kim, Dae-Sik Chang, Sung-Ryul Huh   +4 more
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Development of Calorimeter Target for Long Pulse High Power Negative Ion Source Test Platform

南方能源建设
[Introduction] High energy, strong current, and long pulse neutral beam negative ion source technology are one of the core technologies for achieving combustion conditions in magnetic confinement fusion reactor plasma.
Hongxia ZHOU, Yinxiang WAN, Bowen ZHOU, Peixuan YU, Peiqin TANG, Huiling WEI   +5 more
doaj   +1 more source

Modeling of Beam Acceleration for the Negative Ion Source NIO1

, 2017
The RF negative ion source NIO1 (Negative Ion Optimization 1) [1], built at Consorzio RFX in Padova (Italy), aims at investigating basic issues of ion source physics while providing a tool to benchmark and validate beam simulation codes. Due to its small
Pimazzoni Antonio, Veltri Pierluigi, Cavenago Marco, Serianni Gianluigi   +3 more
core   +1 more source

Recent insights into the molecular mechanism of ubiquinol oxidation by cytochrome bc1

FEBS Open Bio, EarlyView.
The review reflects on the mechanism of the catalytic reaction in cytochrome bc1: the electron bifurcation that involves the separation of two electrons derived from the quinol oxidation to opposite sides of the enzyme across the membrane. This review summarizes the long‐standing effort to understand the mechanism of quinol oxidation catalyzed by ...
Anna Wójcik‐Augustyn, Artur Osyczka, Marcin Sarewicz   +2 more
wiley   +1 more source