Results 201 to 210 of about 436,057 (310)

Exploring the Impact of Child Opportunity on PICU Utilization and the Role of Public Insurance. [PDF]

Crit Care Explor
Myers CN, Duan Q, Magee PM, Pitkowsky Z, Riley CL, Beck AF.   +5 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

hoodscanR: profiling single-cell neighborhoods in spatial transcriptomics data. [PDF]

Bioinformatics
Liu N, Martin J, Bhuva DD, Chen J, Li M, Lee SC, Kharbanda M, Cheng J, Mohamed A, Kulasinghe A, Chen Y, Tan CW, Li F, Polo JM, Davis MJ.   +14 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Built Environment Audits Across Space and Time for Estimating Time-Varying Exposures: Cohort Study. [PDF]

JMIR Form Res
Wang F, Xu X, Ghias E, Mooney SJ, Rundle AG, Sowa WA, Henry K, Stroup A, Harris G, Li J, Stanich PP, Hampel H, Plascak JJ.   +12 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Long-Term Industrial Emissions Exposure and Cognitive Impairment in Older U.S. Adults: A Longitudinal Cohort Study. [PDF]

Health Sci Rep
Husted GE, Cassels SL, Ackert ES, Bendlin BB.   +3 more
europepmc   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Neighborhood Socioeconomic Status and Heart Failure or All-Cause Readmission Within a Municipal Hospital System. [PDF]

JACC Adv
Jeon D, Durstenfeld MS, Ma Y, Thakkar AB, Hsue PY.   +4 more
europepmc   +1 more source