Results 21 to 30 of about 230,179 (219)

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source

Identification of A p300–SP1–BRD4 Transcriptional Axis as a Key Driver of AR Hyperactivation in Polycystic Ovarian Syndrome

Advanced Science, EarlyView.
This study reveals a pivotal epigenetic regulatory role for the histone acetyltransferase p300, demonstrating that its acetylation of histone 3 at lysine 18 and 27 (H3K18ac and H3K27ac), alongside the formation of the p300/BRD4/SP1 complex, drives AR activation and ovarian fibrosis in PCOS.
Zhengquan Zhu, Yihan Wang, Haiyun Chen, Xinye Yu, Tingyu Wang, Yajing Weng, Meihong Guo, Ying Huang, Gaojian Tao, Wangsen Cao, Yong Wang, Daojuan Wang   +11 more
wiley   +1 more source

Integrative Approaches to Treating Cellular Senescence in Kidney Disease

Advanced Science, EarlyView.
ABSTRACT Cellular senescence in the kidney plays a crucial role in the progression of acute kidney injury and chronic kidney disease. Therapeutic approaches targeting senescent cells, such as small molecule senolytic and senomorphic drugs, display efficacy in preclinical models.
Tomoka Misawa, Amruta A, LaTonya J. Hickson, Joy Wolfram   +3 more
wiley   +1 more source

TRAP5 Inhibition Targeting Scar‐Associated Macrophages Ameliorates Acute Kidney Injury to Chronic Kidney Disease Transition

Advanced Science, EarlyView.
This study delineates macrophage heterogeneity along the acute kidney injury to chronic kidney disease transition. Single‐cell RNA sequencing reveals a TRAP5+ scar‐associated macrophage subset driven by Spp1–Cd44 signaling and mitochondrial metabolic reprogramming.
Chenxi Wang, Yaodong Gu, Wen Du, Lin Xie, Jinwei Quan, Yu Zhao, Ye Cheng, Zhaonan Wei, Yuanyuan Sha, Yi Wang, Dechao Xu, Xiang Gao, Min Chen, Xiangchen Gu   +13 more
wiley   +1 more source

Multimodal AI‐Driven Identification of Dehydrocostus Lactone as a Potent Renal Fibrosis Attenuator Targeting IQGAP1

Advanced Science, EarlyView.
Renal fibrosis, a hallmark of CKD, lacks effective treatments. Herein, we developed a multimodal AI model (TCM‐SPred) to identify anti‐fibrotic agents and found that dehydrocostus lactone (DCL) targets IQGAP1 to inhibit Wnt signaling, blocking the interaction between IQGAP1 and CCT3, demonstrating potent anti‐fibrotic activity in vitro and in vivo ...
Weijiang Lin, Wenzhuo Xu, Kang Liu, Ping Wang, Zhenzhen Zhu, Wenyu Lu, Zhe Zheng, Xiaoqian Peng, Xunkai Yin, Shulan Mei, An Pan, Jian Liu, Lihong Hu   +12 more
wiley   +1 more source

A Reflection on the Use of Additive Manufacturing in Nephrology for Education and Surgical Planning

Apollo Medicine, 2020
Additive manufacturing (AM) or three-dimensional printing (3DP) is a new technology known for rapid fabrication of customized or finely detailed parts with decreased cost.
Azhar Equbal, Shahid Akhtar, Md. Asif Equbal   +2 more
doaj   +1 more source

Endogenous Amplification of Apoptosis via p53 Regulation using a Cascade Nanocatalytic Medicine

Advanced Science, EarlyView.
An engineered MOF nanomedicine (MAL) triggers cascade nanocatalysis in tumors, converting H2O2 and L‐Arg into cytotoxic peroxynitrite. This induces DNA damage, activates the p53 pathway, and amplifies mitochondrial apoptosis via the BAX/Bcl‐2/Caspase‐3 axis, effectively inhibiting colorectal cancer growth with high biocompatibility.
Tan Wu, Xiaoyue Xu, Dan Xu, Hui Zhang, Nan Wang, Yang Yang, Qian Chen, Shunjie Chen, Cheng Li   +8 more
wiley   +1 more source

Deciphering the Impact of RAC1‐SPTAN1 in ARPKD Cystogenesis Using Multifaceted Models

Advanced Science, EarlyView.
Distal/connecting tubules expressing SLC8A1 have been suggested as a potential origin of ARPKD cysts. SPTAN1 has been identified as a key molecule in ARPKD cyst formation. Restoring SPTAN1 in PKHD1−/− organoids reduced cyst formation, normalized calcium levels, and decreased RAC1/c‐FOS expression, highlighting SPTAN1's role in ARPKD and the potential ...
Shohei Kuraoka, Yuhei Higashi, Suguru Saito, Solmaz Pourgonabadi, Honami Honjoh, Sho Ishigaki, Peter C. Harris, Lisa M. Satlin, Michifumi Yamashita, Ryuji Morizane   +9 more
wiley   +1 more source

Lysozyme nephropathy in chronic myelomonocytic leukemia

Clinical Case Reports, 2019
Lysozyme nephropathy is a frequently unrecognized cause of renal disease in chronic myelomonocytic leukemia and may serve as a novel indication for treatment in this patient population.
Ami B. Patel, Rodney R. Miles, Michael W. Deininger   +2 more
doaj   +1 more source

Integrating Artificial Intelligence With Droplet‐Based Microfluidics: Advances, Challenges, and Emerging Opportunities

Advanced Intelligent Systems, EarlyView.
Droplet‐based microfluidics enables precise, high‐throughput microscale reactions but continues to face challenges in scalability, reproducibility, and data complexity. This review examines how artificial intelligence enhances droplet generation, detection, sorting, and adaptive control and discusses emerging opportunities for clinical and industrial ...
Junyan Lai, Sukrit Gaira, Rongfeng Wang, Qingtian Zhang, Ming Li, Liao Wu, Diangeng Li, Yi Li, Chengchen Zhang, Jinhong Guo, Shi‐Yang Tang   +10 more
wiley   +1 more source