Results 171 to 180 of about 142,834 (335)

Multiplex Nested PCR for Vibrio Cholera v1

Primers targeting various regions of the V. Cholera genome were obtained from the literature or designed, and optimized for a multiplex nested PCR assay. The following primers were extracted from Hoshino et al., 1998: O1_rfbFor_Inner (modified here by Dr Alex Shaw), O1_rfbRev_Inner, O139_rfbFor_Inner, O139_rfbRev_Outer.
Shannon Fitz, Alex Shaw, Dilip Abraham, michael Owusu   +3 more
openaire   +1 more source

Cas12Fold Accurately Predicts Cas12 Nuclease Structures to Enable Structure‐based Genome‐editing Engineering

Small Structures, EarlyView.
Cas12Fold, a deep‐learning framework tailored to Cas12 nucleases, uses evolutionary signals and iterative structure‐based alignment to resolve conformational complexity. This method outperforms general models in predicting key domains and states, accurately modeling enzymes such as Casλ.
Feng Xu, Zilong Zhao, Chang Liu, Meixia Yu, Ke Li, Yilin Jing, Peiyang Li, Beibei Xin, Jian Chen, Lizhu E, Jinsheng Lai, Zhijia Yang, Hainan Zhao   +12 more
wiley   +1 more source

Phylogeny and historical biogeography of the Pachira sensu lato clade (Bombacoideae, Malvaceae) with a new infrageneric classification

TAXON, EarlyView.
Abstract We explore phylogenetic relationships within the Pachira sensu lato clade (Bombacoideae, Malvaceae), test the monophyly of the genera Eriotheca and Pachira, and investigate their biogeographic history. The Pachira s.l. clade comprises ca. 72 species that traditionally have been placed in Eriotheca and Pachira. We sampled ca. 79% of its species
Vania Nobuko Yoshikawa, Júlia Sousa Siqueira, William S. Alverson, Rafaela Passos Camargo, Alexandre Wagner Silva Hilsdorf, Letícia Rafaela de Morais, Flávia Fonseca Pezzini, Marília Cristina Duarte   +7 more
wiley   +1 more source

Sensitive poliovirus detection using nested PCR and nanopore sequencing: a prospective validation study. [PDF]

Nat Microbiol, 2023
Shaw AG, Mampuela TK, Lofiko EL, Pratt C, Troman C, Bujaki E, O'Toole Á, Akello JO, Aziza AA, Lusamaki EK, Makangara JC, Akonga M, Lay Y, Nsunda B, White B, Jorgensen D, Pukuta E, Riziki Y, Rankin KE, Rambaut A, Ahuka-Mundeke S, Muyembe JJ, Martin J, Grassly NC, Mbala-Kingebeni P.   +24 more
europepmc   +1 more source

Detection of Cryptosporidium parvum DNA in human feces by nested PCR [PDF]

, 1996
A B Balatbat, George W. Jordan, Y J Tang, J Silva   +3 more
openalex   +1 more source

A novel circumscription and classification for Neurolaeneae (Asteraceae)

TAXON, EarlyView.
Abstract Neurolaeneae (Asteraceae) have a complicated taxonomic history. The tribe was described in 1927, but subsequently disregarded in 1977, and later reinstated as a tribe following a large‐scale phylogenetic analysis of Asteraceae in 2002. To date, this tribe has remained poorly studied and it has never been the subject of a comprehensive ...
Vinicius R. Bueno, Morgan R. Gostel, Genilson A. Reis‐Silva, Jimi N. Nakajima, Gustavo Heiden   +4 more
wiley   +1 more source

Nested PCR Approach for petB Gene Metabarcoding of Marine Synechococcus Populations. [PDF]

Microbiol Spectr, 2023
Ong DRY, Gutiérrez-Rodríguez A, Garczarek L, Marie D, Lopes Dos Santos A.   +4 more
europepmc   +1 more source

An improved phylogeny and revised taxonomy of Catillariaceae (Lecanoromycetes, Ascomycota)

TAXON, EarlyView.
Abstract The boundaries of the family Catillariaceae have largely remained untested through phylogenetic methods. Recent studies have led to the transfer of several genera previously classified in Catillariaceae to the newly described family Leprocaulaceae. Despite these changes, the distinction between Catillariaceae, Leprocaulaceae, and Catinariaceae
Måns Svensson, Stefan Ekman, Martin Westberg   +2 more
wiley   +1 more source

DETECTION OF NEWCASTLE DISEASE VIRUS BY NESTED REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION

Jurnal Veteriner, 2008
A study to utilize nested reverse trancriptase-polymerase chain reaction (RT-PCR) for the detection ofNewcastle disease virus (NDV) infection was carried out.
Anak Agung Ayu Mirah Adi, Nyoman Mantik Astawa, Ketut Santhia Adhy Putra, Yasunobu Matsumoto   +3 more
doaj  

New Recording of Toxoplasma gondii in Wild Tortoise Testudo graeca Using Nested PCR Method. [PDF]

Arch Razi Inst, 2023
Aziz Anah S, Aziz Anah S.
europepmc   +1 more source