Results 311 to 320 of about 142,834 (335)

Nested PCR for the Detection of Cryptosporidium parvum

Parasitology Today, 1999
In their recent review, Morgan and Thompson1xMorgan, U.M. and Thompson, R.C.A. Parasitol. Today. 1998; 14: 241–245Abstract | Full Text | Full Text PDF | PubMed | Scopus (48)See all References1 described the pitfalls of using nested PCR for the detection of the diarrhoea-causing protozoan, Cryptosporidium parvum, in faecal samples or in waste or surface
C L, Gibbons, F M, Awad-El-Kariem
openaire   +2 more sources

Application of nested PCR for diagnosis of histoplasmosis

Journal of Infection and Chemotherapy, 2013
Histoplasmosis is a fungal infection that, although not endemic in Japan, has seen a rise in the number of Japanese cases since the mid-1980s. Diagnosis of the disease is not straightforward, and the main method of detection, fungal culture (which has biosafety-related issues), is of low sensitivity in general.
Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Yukihiro Kaneko, Satoshi Yamagoe, Yoshitsugu Miyazaki, Hideaki Ohno, Koichi Tanabe, Takashi Umeyama, Satoshi Yamagoe   +9 more
openaire   +2 more sources

Detection of Brucella Abortus Using Nested-PCR Molecular Method

Clinical Laboratory, 2021
Common detection methods of brucellosis are always accompanied by technical problems in infection diagnosis. In this study, the nested PCR technique was used for the detection of Brucella abortus, and it was compared with other methods.This case-control study was done in Kurdistan.
Fatemeh, Keshavarzi, Zahra, Abdolmohammadi   +1 more
openaire   +2 more sources

Rapid HLA‐DRB1 genotyping by nested PCR amplification

Tissue Antigens, 1992
Abstract: State of the art genotyping of HLA class II alleles with group‐specific DNA amplification by the polymerase chain reaction (PCR) (1) and subsequent probing with sequence‐specific oligonucleotides (2–4) is not suitable for typing cadaveric organ donors since the typing procedure takes far more than one working day.
G, Bein, R, Gläser, H, Kirchner
openaire   +2 more sources

A rapid hemi-nested PCR for HTLV-I detection

Journal of Clinical Virology, 2003
A hemi-nested PCR approach was adopted to detect HTLV-1 infection in clinical samples of peripheral blood mononuclear cells (PBMCs) from subjects with positive or indeterminate serological results. Our results showed that the hemi-nested PCR quickly solved the diagnostic query, detecting the presence of proviral HTLV-1 DNA in two of the 252 patients ...
Davide Gibellini, Paola Monari, Monica Sassi, Maria Carla Re   +3 more
openaire   +2 more sources

Single bovine sperm sex typing by amelogenin nested PCR

Theriogenology, 2008
Sex-sorted bovine semen has become a valuable tool in animal production for sex preselection. Development of novel sperm sexing technologies, or evaluation of the quality of existing methods, often requires a single-sperm, sex-typing method that is reliable and easy to perform.
A, Colley, M, Buhr, S P, Golovan
openaire   +2 more sources

Detection of Schistosoma mansoni in Biomphalaria Using Nested PCR

The Journal of Parasitology, 1997
A nested polymerase chain reaction (PCR) protocol was developed for detecting the presence of Schistosoma mansoni sporocysts in intermediate host snails of the genus Biomphalaria. To accomplish this, rDNA genes encoding the 18S rRNA of S. mansoni and Biomphalaria alexandrina from Egypt were sequenced, as were 18S-encoding genes of the 13-16-R1 and ...
B, Hanelt, C M, Adema, M H, Mansour, E S, Loker   +3 more
openaire   +2 more sources

Nested PCR ve Kullanım Alanları

2013
Nested PCR, PCR tekniğinin hassasiyetini artırmak için geliştirilen en popular yöntemlerden biridir. İstenilen DNA dizisinin bulunup çoğaltılmasında hassasiyeti 104 kat artırdığı tahmin edilmektedir. Bu reaksiyon mekanizmasında birbirini takip eden iki polimeraz zincir reaksiyonunu içermektedir. İlk amplifikasyonda hedef DNA’nın dış bölgesine özgün iki
SEVİNDİK, Emre, ABACI, Z. Tuğba
openaire   +1 more source

NESTED-PCR ASSAYS FOR DETECTION OF PHYTOPLASMAS IN STRAWBERRY

Acta Horticulturae, 1997
Flower and fruit virescence and phyllody are known to be associated with the presence of phytoplasmas in symptomatic tissues. During surveys in North and South Italy symptomatic and asymptomatic strawberry samples were examined by nested polymerase chain reaction (PCR) to verify the presence of phytoplasmas using 16S ribosomal DNA primers.
Bertaccini A., Vibio M., Pastore M., Recupero S., Guerrini S., Grimaldi D.   +5 more
openaire   +2 more sources

Lariat-Dependent Nested PCR for Flanking Sequence Determination

2010
Methods detailed in this chapter relate to the use of Lariat-dependent Nested (LaNe) PCR to characterize unknown RNA or DNA sequence flanking known regions. A multitude of approaches designed to determine flanking sequences have been described in the literature.
openaire   +2 more sources