Results 91 to 100 of about 2,106,515 (290)

Fear and Loathing on the Margins of Empire: Socio-religious perspectives connecting the Netherlands and South Africa before and into the South African War

open access: yesRevista de História da Sociedade e da Cultura
Focusing especially on writings by the theologian Abraham Kuyper and the impact of two Dutch born clergymen with substantial careers in South Africa’s Dutch Reformed Church in the late 19th century, this article develops a perspective on the intertwined
Retief Muller
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Kimya Öğretmeni Yetiştirme Programlarının Karşılaştırılması: Türkiye ve Hollanda Örneği

open access: yesVan Yüzüncü Yıl Üniversitesi Eğitim Fakültesi Dergisi
Bu karşılaştırmalı eğitim araştırmasında, Türkiye’de Hacettepe Üniversitesi ve Hollanda’da Hogeschool Utrecht Kimya Öğretmeni yetiştirme programlarının öğretmen yetiştirme sistemi, zorunlu alan eğitimi dersleri, zorunlu öğretmenlik meslek bilgisi ...
Sinem Dinçol Özgür   +2 more
doaj  

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

open access: yesFEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara   +3 more
wiley   +1 more source

Religious Autobiographies in the Netherlands: Authors, Publishers and Readers, 1750–1950

open access: yesEuropean Journal of Life Writing, 2018
In this article more than 200 religious autobiographies written by Dutch  orthodox pietist men and women are analyzed. Although hardly studied so far, these texts were a substantial part of all printed Dutch egodocuments,  especially in the period 1850 ...
Arianne Baggerman, Rudolf Dekker
doaj   +1 more source

THE NETHERLANDS

open access: yes, 2019
Contains fulltext : 206826.pdf (Publisher’s version ) (Open Access)
openaire   +2 more sources

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Home - About - Disclaimer - Privacy