Results 231 to 240 of about 17,973 (254)
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Spontaneous apoptosis in murine free-floating neurospheres
Experimental Cell Research, 2004We have shown successful in vitro expansion of rodent and human neural precursor cells (NPC) derived from fetal midbrain and forebrain. Here, we show that mouse neural precursor cells growing in neurospheres proliferate, but also undergo spontaneous apoptosis in vitro. On average, 30.7 +/- 3.4% cells of midbrain-derived neural precursors and 32.1 +/- 2.
Javorina, Milosevic +2 more
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Regional specification of rodent and human neurospheres
Developmental Brain Research, 2002Neural precursor cells were isolated from various regions of the developing rat and human brain and grown in culture as aggregates termed neurospheres. We asked whether cells within human and rodent neurospheres are identical, or whether they have species specific characteristics or differences based on their region of origin.
T, Ostenfeld +6 more
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Imaging of neurosphere oxygenation with phosphorescent probes
Biomaterials, 2013Multicellular spheroids are useful models of mammalian tissue for studies of cell proliferation, differentiation, replacement therapies and drug action. Having a size of 100-500 μm they mimic in vivo micro-environment and characteristic gradients of O2, pH and nutrients.
Ruslan I. Dmitriev +3 more
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Simulation of the growth of neurospheres
Europhysics Letters (EPL), 2004Under appropriate conditions, the neural stem cells are able to proliferate and to form suspended neurospheres containing stem cells and neuronal and glial progenitors in different stages of differentiation. Our lattice Monte Carlo simulations, taking into account the effect of spatial constraints and cell-cell adhesion on cell diffusion and division ...
V. P Zhdanov, B Kasemo
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Growth hormone promotes proliferation of adult neurosphere cultures
Growth Hormone & IGF Research, 2009Growth hormone (GH) and its receptor (GHR) are widely expressed in the CNS. During development, GH signaling regulates both proliferation of neural progenitor cells as well as their differentiation into neurons and glia. Here we have examined the effect of GH signaling on adult subventricular zone derived neural progenitor cells cultured as ...
McLenachan, S +3 more
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Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay
Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine.In this chapter, we describe a ...Alfredo González-Gil, Rosa Ana Picazo
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Neurospheres: Insights into neural stem cell biology
Journal of Neuroscience Research, 2004AbstractNeural stem cells (NSC) are a tissue‐specific subtype of self‐renewing and multipotent cells that can give rise to all neural populations. In this review, the importance of maintaining cell–cell contacts in the study of NSC is highlighted, and data obtained from some crucial single‐cell studies is compared to results obtained from neurospheres,
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Differentiation of neurospheres from the enteric nervous system
Pediatric Surgery International, 2003The enteric nervous system (ENS) derives from neural crest cells, which migrate from the neural tube into the developing gut. The neuronal and glial precursor cells migrate mainly from the oral towards the anal end of the gastrointestinal tract. So far, knowledge about the multipotent influences upon the ENS development, especially its neurotrophic ...
Karl-Herbert, Schäfer +2 more
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Metabolic properties of astrocytes differentiated from rat neurospheres
Brain Research, 2006The metabolic properties of astroglia differentiated from neurospheres have not been fully assessed. In this study, the glycolytic and oxidative metabolism of glucose in astroglia differentiated from rat tertiary neurospheres (astroglia(NS)) was compared with that in astroglia prepared from the striata of embryonic day 16 rats (astroglia(ST)).
Takato, Abe +2 more
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A protocol for immunofluorescence staining of floating neurospheres
Neuroscience Letters, 2010This protocol describes the immunofluorescence staining of floating neurospheres in culture plates. Although this protocol is similar to conventional immunofluorescence staining, the staining procedure of floating neurospheres in multiwell culture plates and the washing procedure are different.
Ryo, Sasaki +7 more
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