Results 261 to 270 of about 22,400 (295)
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Genome-wide pooled CRISPR screening in neurospheres
Nature Protocols, 2023Spheroid culture systems have allowed in vitro propagation of cells unable to grow in canonical cell culturing conditions, and may capture cellular contexts that model tumor growth better than current model systems. The insights gleaned from genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screening of thousands of cancer ...
Tanaz Abid+12 more
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Retinal neurospheres prepared as tissue for transplantation
Developmental Brain Research, 2005The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of ...
Ingela Liljekvist-Larsson+1 more
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Journal of drug targeting (Print), 2018
Glioblastomas (GBMs) are known to harbour subsets of cells known as tumour-initiating cells (TICs), which are responsible for the maintenance, invasiveness and recurrence of GBMs.
Aditi Jhaveri, E. Luther, V. Torchilin
semanticscholar +1 more source
Glioblastomas (GBMs) are known to harbour subsets of cells known as tumour-initiating cells (TICs), which are responsible for the maintenance, invasiveness and recurrence of GBMs.
Aditi Jhaveri, E. Luther, V. Torchilin
semanticscholar +1 more source
Neurosphere segmentation in brightfield images
SPIE Proceedings, 2014The challenge of segmenting neurospheres (NSPs) from brightfield images includes uneven background illumination (vignetting), low contrast and shadow-casting appearance near the well wall. We propose a pipeline for neurosphere segmentation in brightfield images, focusing on shadow-casting removal.
Wei Xiong+5 more
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ACS Chemical Neuroscience, 2018
Reconstitution of a complex biological structure or system following a simple and facile strategy using minimum physiochemical cues is challenging for an in-depth understanding of the system. In particular, the brain is a highly sophisticated and complex
Juhee Khan+5 more
semanticscholar +1 more source
Reconstitution of a complex biological structure or system following a simple and facile strategy using minimum physiochemical cues is challenging for an in-depth understanding of the system. In particular, the brain is a highly sophisticated and complex
Juhee Khan+5 more
semanticscholar +1 more source
Isolation and culture of adult neurons and neurospheres
Nature Protocols, 2007Here we present a protocol for extraction and culture of neurons from adult rat or mouse CNS. The method proscribes an optimized protease digestion of slices, control of osmolarity and pH outside the incubator with Hibernate and density gradient separation of neurons from debris.
Gregory J. Brewer, John R. Torricelli
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Stigmasterol promotes neuronal migration via reelin signaling in neurosphere migration assays
Nutritional neuroscience, 2018Stigmasterol (ST) is a multifunctional phytosterol and is found in diverse food. In our previous transcriptomics study, we found ST upregulated migration-related genes.
Md. Nazmul Haque, I. Moon
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A protocol for immunofluorescence staining of floating neurospheres
Neuroscience Letters, 2010This protocol describes the immunofluorescence staining of floating neurospheres in culture plates. Although this protocol is similar to conventional immunofluorescence staining, the staining procedure of floating neurospheres in multiwell culture plates and the washing procedure are different.
Keiji Wada+7 more
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Regional specification of rodent and human neurospheres
Developmental Brain Research, 2002Neural precursor cells were isolated from various regions of the developing rat and human brain and grown in culture as aggregates termed neurospheres. We asked whether cells within human and rodent neurospheres are identical, or whether they have species specific characteristics or differences based on their region of origin.
Clive N. Svendsen+6 more
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Neurospheres as a Model for Developmental Neurotoxicity Testing
2011Developmental neurotoxicity (DNT) of environmental chemicals is a serious threat to human health. So far, DNT testing is performed in animals. Such in vivo testing is time-consuming, expensive, and uses large numbers of animals. Moreover, species differences (rat-human) bear the problem of extrapolation.
Kathrin Gassmann+3 more
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