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Strengths and Limitations of the Neurosphere Culture System [PDF]

Molecular Neurobiology, 2006
After the initial reports of free-floating cultures of neural stem cells termed neurospheres (1,2), a wide array of studies using this promising culture system emerged. In theory, this was a near-perfect system for large-scale production of neural cells for use in cell replacement therapies and to assay for and characterize neural stem cells. More than
Malin Parmar, Josephine B. Jensen
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Genome-wide pooled CRISPR screening in neurospheres

Nature Protocols, 2023
Spheroid culture systems have allowed in vitro propagation of cells unable to grow in canonical cell culturing conditions, and may capture cellular contexts that model tumor growth better than current model systems. The insights gleaned from genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screening of thousands of cancer ...
Tanaz Abid, Amy B. Goodale, Zohra Kalani, Meghan Wyatt, Elizabeth M. Gonzalez, Kevin Ning Zhou, Kenin Qian, Dana Novikov, Alexandra-Larisa Condurat, Pratiti Bandopadhayay, Federica Piccioni, Nicole S. Persky, David E. Root   +12 more
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Retinal neurospheres prepared as tissue for transplantation

Developmental Brain Research, 2005
The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of ...
Ingela Liljekvist-Larsson, Kjell Johansson   +1 more
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The effect of transferrin-targeted, resveratrol-loaded liposomes on neurosphere cultures of glioblastoma: implications for targeting tumour-initiating cells

Journal of drug targeting (Print), 2018
Glioblastomas (GBMs) are known to harbour subsets of cells known as tumour-initiating cells (TICs), which are responsible for the maintenance, invasiveness and recurrence of GBMs.
Aditi Jhaveri, E. Luther, V. Torchilin
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A protocol for immunofluorescence staining of floating neurospheres

Neuroscience Letters, 2010
This protocol describes the immunofluorescence staining of floating neurospheres in culture plates. Although this protocol is similar to conventional immunofluorescence staining, the staining procedure of floating neurospheres in multiwell culture plates and the washing procedure are different.
Keiji Wada, Teruo Okano, Tomohiro Ando, Hideki Ogiuchi, Hiroto Uchiyama, Masayuki Yamato, Ryo Sasaki, Shunsuke Aoki   +7 more
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Neurosphere Development from Hippocampal and Cortical Embryonic Mixed Primary Neuron Culture: A Potential Platform for Screening Neurochemical Modulator.

ACS Chemical Neuroscience, 2018
Reconstitution of a complex biological structure or system following a simple and facile strategy using minimum physiochemical cues is challenging for an in-depth understanding of the system. In particular, the brain is a highly sophisticated and complex
Juhee Khan, Gaurav Das, Varsha Gupta, Saswat Mohapatra, Subhajit Ghosh, Surajit Ghosh   +5 more
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Neurosphere segmentation in brightfield images

SPIE Proceedings, 2014
The challenge of segmenting neurospheres (NSPs) from brightfield images includes uneven background illumination (vignetting), low contrast and shadow-casting appearance near the well wall. We propose a pipeline for neurosphere segmentation in brightfield images, focusing on shadow-casting removal.
Wei Xiong, Joo-Hwee Lim, Sohail Ahmed, Shue Ching Chia, Shvetha Sankaran, Jierong Cheng   +5 more
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Stigmasterol promotes neuronal migration via reelin signaling in neurosphere migration assays

Nutritional neuroscience, 2018
Stigmasterol (ST) is a multifunctional phytosterol and is found in diverse food. In our previous transcriptomics study, we found ST upregulated migration-related genes.
Md. Nazmul Haque, I. Moon
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Regional specification of rodent and human neurospheres

Developmental Brain Research, 2002
Neural precursor cells were isolated from various regions of the developing rat and human brain and grown in culture as aggregates termed neurospheres. We asked whether cells within human and rodent neurospheres are identical, or whether they have species specific characteristics or differences based on their region of origin.
Clive N. Svendsen, Anna L. Peters, T Ostenfeld, Yu-Tzu Tai, Etienne Joly, Eric Jauniaux, Maeve A. Caldwell   +6 more
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Neurospheres as a Model for Developmental Neurotoxicity Testing

2011
Developmental neurotoxicity (DNT) of environmental chemicals is a serious threat to human health. So far, DNT testing is performed in animals. Such in vivo testing is time-consuming, expensive, and uses large numbers of animals. Moreover, species differences (rat-human) bear the problem of extrapolation.
Kathrin Gassmann, Ellen Fritsche, Ellen Fritsche, Timm Schreiber   +3 more
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