Results 261 to 270 of about 22,400 (295)
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Genome-wide pooled CRISPR screening in neurospheres

Nature Protocols, 2023
Spheroid culture systems have allowed in vitro propagation of cells unable to grow in canonical cell culturing conditions, and may capture cellular contexts that model tumor growth better than current model systems. The insights gleaned from genome-wide clustered regularly interspaced short palindromic repeat (CRISPR) screening of thousands of cancer ...
Tanaz Abid   +12 more
openaire   +3 more sources

Retinal neurospheres prepared as tissue for transplantation

Developmental Brain Research, 2005
The present work was conducted to study the cellular composition and developmental capacity of retinal neurospheres. Furthermore, the ability of grafted neurospheres to integrate into adult retinal tissue was studied in an in vitro model. Retinal progenitor cells isolated from rat embryos were expanded into neurospheres in vitro in the presence of ...
Ingela Liljekvist-Larsson   +1 more
openaire   +3 more sources

The effect of transferrin-targeted, resveratrol-loaded liposomes on neurosphere cultures of glioblastoma: implications for targeting tumour-initiating cells

Journal of drug targeting (Print), 2018
Glioblastomas (GBMs) are known to harbour subsets of cells known as tumour-initiating cells (TICs), which are responsible for the maintenance, invasiveness and recurrence of GBMs.
Aditi Jhaveri, E. Luther, V. Torchilin
semanticscholar   +1 more source

Neurosphere segmentation in brightfield images

SPIE Proceedings, 2014
The challenge of segmenting neurospheres (NSPs) from brightfield images includes uneven background illumination (vignetting), low contrast and shadow-casting appearance near the well wall. We propose a pipeline for neurosphere segmentation in brightfield images, focusing on shadow-casting removal.
Wei Xiong   +5 more
openaire   +2 more sources

Neurosphere Development from Hippocampal and Cortical Embryonic Mixed Primary Neuron Culture: A Potential Platform for Screening Neurochemical Modulator.

ACS Chemical Neuroscience, 2018
Reconstitution of a complex biological structure or system following a simple and facile strategy using minimum physiochemical cues is challenging for an in-depth understanding of the system. In particular, the brain is a highly sophisticated and complex
Juhee Khan   +5 more
semanticscholar   +1 more source

Isolation and culture of adult neurons and neurospheres

Nature Protocols, 2007
Here we present a protocol for extraction and culture of neurons from adult rat or mouse CNS. The method proscribes an optimized protease digestion of slices, control of osmolarity and pH outside the incubator with Hibernate and density gradient separation of neurons from debris.
Gregory J. Brewer, John R. Torricelli
openaire   +3 more sources

Stigmasterol promotes neuronal migration via reelin signaling in neurosphere migration assays

Nutritional neuroscience, 2018
Stigmasterol (ST) is a multifunctional phytosterol and is found in diverse food. In our previous transcriptomics study, we found ST upregulated migration-related genes.
Md. Nazmul Haque, I. Moon
semanticscholar   +1 more source

A protocol for immunofluorescence staining of floating neurospheres

Neuroscience Letters, 2010
This protocol describes the immunofluorescence staining of floating neurospheres in culture plates. Although this protocol is similar to conventional immunofluorescence staining, the staining procedure of floating neurospheres in multiwell culture plates and the washing procedure are different.
Keiji Wada   +7 more
openaire   +3 more sources

Regional specification of rodent and human neurospheres

Developmental Brain Research, 2002
Neural precursor cells were isolated from various regions of the developing rat and human brain and grown in culture as aggregates termed neurospheres. We asked whether cells within human and rodent neurospheres are identical, or whether they have species specific characteristics or differences based on their region of origin.
Clive N. Svendsen   +6 more
openaire   +3 more sources

Neurospheres as a Model for Developmental Neurotoxicity Testing

2011
Developmental neurotoxicity (DNT) of environmental chemicals is a serious threat to human health. So far, DNT testing is performed in animals. Such in vivo testing is time-consuming, expensive, and uses large numbers of animals. Moreover, species differences (rat-human) bear the problem of extrapolation.
Kathrin Gassmann   +3 more
openaire   +3 more sources

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