Results 271 to 280 of about 22,400 (295)

Extracellular matrix effects on neurosphere cell motility

Experimental Neurology, 2003
There is a paucity of information on the roles of extracellular matrix (ECM) and substrate molecules in general with regard to the growth and differentiation of neural stem and progenitor cells. There are well-established findings of a dense, presumably astrocyte-derived ECM in the persistently neurogenic subependymal zone and its migratory extension ...
Dennis A. Steindler, Sean M. Kearns, Eric D. Laywell, V.K. Kukekov   +3 more
openaire   +3 more sources

Differentiation of neurospheres from the enteric nervous system

Pediatric Surgery International, 2003
The enteric nervous system (ENS) derives from neural crest cells, which migrate from the neural tube into the developing gut. The neuronal and glial precursor cells migrate mainly from the oral towards the anal end of the gastrointestinal tract. So far, knowledge about the multipotent influences upon the ENS development, especially its neurotrophic ...
Karl-Herbert Schäfer, Ulrich Rauch, Cornelia Irene Hagl   +2 more
openaire   +3 more sources

Spontaneous apoptosis in murine free-floating neurospheres

Experimental Cell Research, 2004
We have shown successful in vitro expansion of rodent and human neural precursor cells (NPC) derived from fetal midbrain and forebrain. Here, we show that mouse neural precursor cells growing in neurospheres proliferate, but also undergo spontaneous apoptosis in vitro. On average, 30.7 +/- 3.4% cells of midbrain-derived neural precursors and 32.1 +/- 2.
Johannes Schwarz, Javorina Milosevic, Alexander Storch   +2 more
openaire   +3 more sources

Alginate Encapsulation as a Novel Strategy for the Cryopreservation of Neurospheres

Tissue Engineering Part C: Methods, 2010
Primary cultures of brain cell neurospheres are valuable in vitro models for neurotoxicology and brain cell research. Such applications would greatly benefit from the development of efficient cryopreservation protocols that assure the availability of viable and genetically stable stocks of functional neurospheres. In this work we aimed at developing an
Heiko Zimmermann, Daniela S. Ferreira, Friederike Ehrhart, Paula M. Alves, Catarina Brito, Rita Malpique, Luísa M. Osório   +6 more
openaire   +3 more sources

Neurospheres from Ovarian Cortical Cells in Mammals: Generation In Vitro and Neurosphere Assay

Isolation of neural stem cells/neural progenitor cells (NSC/NPC) from extra-neural origin, or adult stem cells able to specify into NSC/NPC in vitro under particular cell culture conditions, offers an interesting alternative experimental model with prospective benefits in basic research and translational medicine.In this chapter, we describe a ...
Alfredo González-Gil, Rosa Ana Picazo
openaire   +2 more sources

Production of neurospheres from mammalian Müller cells in culture

Neuroscience Letters, 2007
In lower vertebrates, like fishes and amphibians, retina is able to self-regenerate whereas Mammalian retina has lost this property. Nevertheless, recently, it has turned out that retinal glial Müller cells were playing a role in neuronal regeneration of the adult rodent retina, in case of acute damages, by dedifferentiating and redifferentiating in ...
Germaine Michel, Julie Monnin, David Hicks, Claudine Versaux-Botteri, Claudine Versaux-Botteri, Nadège Morand-Villeneuve   +5 more
openaire   +4 more sources

The mechanical effects of chemical stimuli on neurospheres

Biomechanics and Modeling in Mechanobiology
The formulation of more accurate models to describe tissue mechanics necessitates the availability of tools and instruments that can precisely measure the mechanical response of tissues to physical loads and other stimuli. In this regard, neuroscience has trailed other life sciences owing to the unavailability of representative live tissue models and ...
Yun-Han, Huang, Roza, Vaez Ghaemi, James, Cheon, Vikramaditya G, Yadav, John M, Frostad   +4 more
openaire   +2 more sources

Neural stem cells and neurospheres—re-evaluating the relationship

Nature Methods, 2005
For most of the past century, the prospect of replacing lost or damaged cells in the central nervous system (CNS) was hampered by the opinion that the adult mammalian CNS was incapable of generating new nerve cells. This belief, like most dogmas, was essentially founded on a lack of experimental evidence to the contrary. The overturning of this 'no new
Reynolds, Brent A., Rietze, Rodney L.
openaire   +3 more sources

Growth hormone promotes proliferation of adult neurosphere cultures

Growth Hormone & IGF Research, 2009
Growth hormone (GH) and its receptor (GHR) are widely expressed in the CNS. During development, GH signaling regulates both proliferation of neural progenitor cells as well as their differentiation into neurons and glia. Here we have examined the effect of GH signaling on adult subventricular zone derived neural progenitor cells cultured as ...
McLenachan, S, Lum, M-G, Waters, MJ, Turnley, AM   +3 more
openaire   +3 more sources

Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping

2013
Cell cultures constitute an important tool for research as a way to reproduce pathological processes in a controlled system. However, the culture of brain-derived cells in monolayer presents significant challenges that obscure the fidelity of in vitro results.
Amanda P. Struckhoff, Luis Del Valle
openaire   +3 more sources