Results 21 to 30 of about 22,400 (295)

Characterization of neurosphere cell phenotypes by flow cytometry [PDF]

Cytometry, 2000
Neural stem cell research regularly utilizes neurosphere cultures as a continuous source of primitive neural cells. Results from current progenitor cell assays show that these cultures contain a low number of neural progenitors. Our goal is to characterize neurosphere cultures and define subpopulations in order to purify neural progenitor cells.Cells ...
Ruud Hulspas, Peter J. Quesenberry
openalex   +4 more sources

537. Further Characterisation of Myo-Neurospheres [PDF]

Molecular Therapy, 2004
Adult stem cells have been isolated from several tissues like bone marrow, brain and skin. We report the isolation and characterisation of neuronal precursor cells, potentially stem cells, from primate and mouse adult muscle and their differentiation in neuronal cells.
Simon Quenneville, Marie-Eve Ducharme, Joël Rousseau, Alain Garnier, Jacques P. Tremblay   +4 more
openalex   +3 more sources

Enteric Neurospheres Are Not Specific to Neural Crest Cultures: Implications for Neural Stem Cell Therapies [PDF]

, 2015
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work ...
A Barlow, A Pini Prato, A Zani, AG Baynash, AG Catto-Smith, AJ Burns, AJ Burns, Alan J. Burns, AS Wallace, C Caldwell, C Kalcheim, C Laranjeira, CH Knowles, Conor McCann, D Natarajan, Dipa Natarajan, DM Rodrigues, E Panza, Ellen Binder, GE Boeckxstaens, H Tsuji, HM Pallari, J Amiel, Jean-Marie Delalande, Julie Cooper, L Becker, L Ludman, M Hanani, M Metzger, Mara Cananzi, MC Nunes, MD Gershon, Mirella Dottori, MP Sanchez, MT Liu, N Bondurand, N Nagoshi, N Thapar, Nikhil Thapar, NR Druckenbrod, PS Danielian, R Hotta, R Hotta, R Zufferey, Rania Kronfli, RC Mifflin, RM Lindley, S Almond, S Bixby, S Hetz, S Srinivas, S Theocharatos, SJ Conway, SJ Howe, SJ McKeown, TA Heanue, TP Rothman, U Rauch, V Sasselli, X Jiang, Y Matsuda, YH Tsai   +61 more
core   +26 more sources

Adherent vs. Free-Floating Neural Induction by Dual SMAD Inhibition for Neurosphere Cultures Derived from Human Induced Pluripotent Stem Cells [PDF]

Front. Cell Dev. Biol., 2018
Keeping neural stem cells under proliferation, followed by terminal differentiation, can substantially increase the number of neurons generated. With regard to the usability of proliferating neurospheres (NSPHs) cultures, adherent induction protocols ...
Martje G. Pauly, Victor Krajka, Felix Stengel, Philip Seibler, Christine Klein, Philipp Capetian   +5 more
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Neurosphere and adherent culture conditions are equivalent for malignant glioma stem cell lines [PDF]

Anatomy & Cell Biology, 2015
Certain limitations of the neurosphere assay (NSA) have resulted in a search for alternative culture techniques for brain tumor-initiating cells (TICs). Recently, reports have described growing glioblastoma (GBM) TICs as a monolayer using laminin.
Maryam Rahman, Karina Reyner, Loic P. Deleyrolle, Sebastien Millette, Hassan Azari, Bryan W. Day, Brett W. Stringer, Andy Boyd, Terrance G. Johns, Vincent Blot, Rohit Duggal, Brent A. Reynolds   +11 more
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Establishment and phenotyping of neurosphere cultures from primary neuroblastoma samples [PDF]

F1000Research, 2019
Background: Primary cell culture using serum free media supplemented with growth factors has been used in a number of cancers to propagate primary cells with stem like properties, which form as spherical cellular aggregates.
Jack Barton, Katherine Pacey, Neha Jain, Tessa Kasia, Darren Edwards, Christine Thevanesan, Karin Straathof, Giuseppe Barone, John Anderson   +8 more
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Accelerating the in vitro emulation of Alzheimer’s disease-associated phenotypes using a novel 3D blood-brain barrier neurosphere co-culture model

Frontiers in Bioengineering and Biotechnology, 2023
High failure rates in clinical trials for neurodegenerative disorders such as Alzheimer’s disease have been linked to an insufficient predictive validity of current animal-based disease models. This has created an increasing demand for alternative, human-
Eunkyung Clare Ko, Sarah Spitz, F. M. Pramotton, Olivia M. Barr, Ciana Xu, Georgios Pavlou, Shun Zhang, Alice Tsai, Anna Maaser-Hecker, Mehdi Jorfi, Se Hoon Choi, Rudolph E. Tanzi, R. Kamm   +12 more
semanticscholar   +1 more source

Valproic acid enhances neurosphere formation in cultured rat embryonic cortical cells through TGFβ1 signaling

Journal of Biomedical Research, 2022
This study aimed to investigate the effect and mechanism of valproic acid (VPA) on the neurosphere formation in rat embryonic cortical cells. We used free-floating neurosphere formation as a model system to evaluate the VPA on the proliferation of neural
C. Qi, Jiaqi Zhang, Yuanyuan Wang, Mingyan Lin, Jun Gao, Haiying Lu   +5 more
semanticscholar   +1 more source

A tenascin-C mimetic peptide amphiphile nanofiber gel promotes neurite outgrowth and cell migration of neurosphere-derived cells

Acta Biomaterialia, 2016
Eric J. Berns, Zaida Álvarez, Joshua E. Goldberger, Job Boekhoven, John A. Kessler, H. Georg Kuhn, Samuel I. Stupp   +6 more
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Prion infection of mouse neurospheres [PDF]

Proceedings of the National Academy of Sciences, 2006
Only a few cell lines have been infected with prions, offering limited genetic diversity and sensitivity to several strains. Here we report that cultured neurospheres expressing cellular prion protein (PrP C ) can be infected with prions. Neurosphere lines isolated from the brains of mice at embryonic day 13–15 grow
George A. Carlson, Stephen J. DeArmond, Stanley B. Prusiner, Ranjit K. Giri, Rose Pitstick, Rebecca L. Young   +5 more
openaire   +3 more sources