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Chitinase from Neurospora crassa
1988Publisher Summary This chapter describes the assay method and purification procedures for chitinase from Neurospora crassa. The enzyme behaves as an cndochitinase; it produces low-molecular-weight, soluble multimers of N-acctyl-D-glucosaminc, the dimer N,N'-diacetylchitobiose being predominant.
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Protease secretion in Neurospora crassa
Biochemical and Biophysical Research Communications, 1974Abstract Secreted and constitutive intracellular proteases of Neurospora crassa differ with regard to inhibitor sensitivity, substrate specificity, isoelectric points and other properties. Upon the induction of protease secretion the enzymes released from the mycelium are formed de novo as demonstrated by density labelling with D2O ...
Ph. Matile, U. Heiniger
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Control of Transcription in Neurospora crassa
1974Gene expression can be assessed by the amounts and types of RNA synthesized at any growth period. No differences were observed in the polynucleotide sequences of DNA from different organs, although large differences were noted among the rapidly labeled RNAs isolated from these tissues (1). The method of molecular hybridization has permitted measurement
P. R. Mahadevan, A. S. Bhagwat
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Vaccine production in Neurospora crassa
Biologicals, 2009We have chosen to use the filamentous fungus Neurospora crassa to produce subunit vaccines. Here we describe the production and purification of Influenza hemagglutinin and neuraminidase antigens in N. crassa. The N. crassa system used by Neugenesis offers many advantages over other systems for production of recombinant protein. In contrast to mammalian
Silke Allgaier+5 more
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Mutagenicity of methylhydroxylamines in Neurospora crassa
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1967Abstract The mutagenicity of hydroxylamine (HA) and 2 hydroxylamine derivatives, methoxylamine (OMHA) and N -methylhydroxylamine (NMHA), have been tested in 22 purple adenine-requiring mutants ( ad-3B ) of Neurospara crassa . Fourteen of the mutants were previously classified as reverting by base-pair substitution and 8 as reverting by base-pair ...
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Rhythmic Conidiation in Neurospora crassa
2007In the filamentous fungus Neurospora crassa the production of asexual spores (conidia) is regulated by its circadian clock. When the fungus is grown on a thin layer of agar medium in long growth tubes (so-called "race tubes"), restricting its growth to one direction only, bright orange bands are clearly visible.
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Mutagenicity of neocarzinostatin in Neurospora crassa
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 1984Neocarzinostatin (NCS) is an acidic, single-chain polypeptide of 109 amino acids that has shown some antitumor activity in clinical trials. NCS is mutagenic in recA+ strains of Escherichia coli, but not in recA strains; on the other hand, a defect in the nucleotide-excision-repair pathway has no effect on the mutagenicity of NCS in E. coli.
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Heterocaryosis in Neurospora crassa
1953It has been shown that heterocaryon formation between certain biochemical mutants of Neurospora crassa is controlled by a number of genes apart from the biochemical mutant genes concerned. Genetic control of heterocaryosis has been shown for several different combinations of mutants.
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Isolation of nuclei of Neurospora crassa
Biochimica et Biophysica Acta, 1961Edward Reich, Seizo Tsuda
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Variations in Neurospora Crassa
1960In the year 1896, E. B. Wilson wrote, “Inheritance is the recurrence in successive generations, of like forms of metabolism.” Yet, because of differences in metabolism, there are probably no two individuals of any species completely identical. Although individuals may have exactly the same genetic complement, if that is ever actually possible, as soon ...
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