Results 241 to 250 of about 46,863 (279)

Redundant DNA of Neurospora crassa

Biochemical Genetics, 1972
Approximately 20% of the DNA of Neurospora crassa consists of redundant sequences. This is calculated from the reassociation rate of fragmented, denatured DNA as measured by hydroxyapatite column chromatography. The redundant DNA has a complexity of 105 base pairs and a repetition frequency of up to 60 copies per genome.
R R, Brooks, P C, Huang
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Inositol biosynthesis in Neurospora crassa

Biochimica et Biophysica Acta (BBA) - General Subjects, 1967
Abstract 1. 1. The biosynthesis of myo -inositol in wild-type Neurospora crassa and in inositol-less mutant 896001 has been studied. 2. 2. Two enzyme preparations capable of synthesizing myo -inositol were obtained, separated and partially purified. One of them, which has been purified 360-fold, is present only in the wild-type strain and
E, Piña, E L, Tatum
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Chitinase from Neurospora crassa

1988
Publisher Summary This chapter describes the assay method and purification procedures for chitinase from Neurospora crassa. The enzyme behaves as an cndochitinase; it produces low-molecular-weight, soluble multimers of N-acctyl-D-glucosaminc, the dimer N,N'-diacetylchitobiose being predominant.
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Protease secretion in Neurospora crassa

Biochemical and Biophysical Research Communications, 1974
Abstract Secreted and constitutive intracellular proteases of Neurospora crassa differ with regard to inhibitor sensitivity, substrate specificity, isoelectric points and other properties. Upon the induction of protease secretion the enzymes released from the mycelium are formed de novo as demonstrated by density labelling with D2O ...
U, Heiniger, P, Matile
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Vaccine production in Neurospora crassa

Biologicals, 2009
We have chosen to use the filamentous fungus Neurospora crassa to produce subunit vaccines. Here we describe the production and purification of Influenza hemagglutinin and neuraminidase antigens in N. crassa. The N. crassa system used by Neugenesis offers many advantages over other systems for production of recombinant protein. In contrast to mammalian
Silke, Allgaier, Rebecca D, Taylor, Yuliya, Brudnaya, David J, Jacobson, Edward, Cambareri, W Dorsey, Stuart   +5 more
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Map Construction in Neurospora Crassa

1954
Publisher Summary In this chapter linkage and centromere data from neurospora crassa have been compiled from all published material, as well as unpublished sources. Tetrad data from gene–centromere and gene–gene intervals have been placed on a uniform basis for mapping by computing map lengths from second-division segregation frequencies and ...
R W, BARRATT, D, NEWMEYER, D D, PERKINS, L, GARNJOBST   +3 more
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Neurospora crassa mitochondrial transfer RNAs

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
Total mitochondrial tRNA from Neurospora crassa was characterized by base composition analysis, one- and two-dimensional gel electrophoreses and reversed-phase chromatography on RPC5. The guanosine + cytidine content was about 43%, as compared to 60% for cytoplasmic tRNA. The modified nucleoside content was low and about the same as that of total yeast
de Vries, Hans, de Jonge, JennyC., Schneller, J.M., Martin, Robert P., Dirheimer, Guy, Stahl, Andre J.C.   +5 more
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Postreplication repair in Neurospora crassa

Molecular and General Genetics MGG, 1982
Changes in the molecular weight of nascent DNA made after ultraviolet (UV) irradiation have been studied in the excision-defective Neurospora mutant uvs-2 using isotopic pulse labeling, alkaline gradient centrifugation and alkaline filter elution. Both the size of nascent DNA and the rate of incorporation of label into DNA was reduced by UV light in a ...
R E, Calza, A L, Schroeder
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Sorbose transport in Neurospora crassa

Biochimica et Biophysica Acta (BBA) - Biomembranes, 1967
Abstract 1. 1.|The transport of sorbose into late log-phase cultures of Neurospora crassa does not occur against a concentration gradient, but obeys saturation kinetics, is energy-requiring, and is competitively inhibited by glucose. 2. 2.|The apparent K m for transport is 116 mM, and the maximal velocity is 0.92 μmol/mg dry wt. per h.
B, Crocken, E L, Tatum
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Rhythmic Conidiation in Neurospora crassa

2007
In the filamentous fungus Neurospora crassa the production of asexual spores (conidia) is regulated by its circadian clock. When the fungus is grown on a thin layer of agar medium in long growth tubes (so-called "race tubes"), restricting its growth to one direction only, bright orange bands are clearly visible.
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