HIV-1 Envelope and MPER Antibody Structures in Lipid Assemblies
Cell Reports, 2020 Summary: Structural and functional studies of HIV envelope glycoprotein (Env) as a transmembrane protein have long been complicated by challenges associated with inherent flexibility of the molecule and the membrane-embedded hydrophobic regions. Here, we
Kimmo Rantalainen +22 more
doaj +1 more source
Nature Communications, 2020 The spread of the SARS-CoV-2 into a global pandemic within a few months of onset motivates the development of a rapidly scalable vaccine. Here, we present a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid ...
P. McKay +14 more
semanticscholar +1 more source
RSV Neutralizing Antibodies in Dried Blood
The Journal of Infectious Diseases, 2023 Abstract Background The key correlate of protection of respiratory syncytial virus (RSV) vaccines and monoclonal antibodies (mAbs) is virus neutralization, measured via sera obtained through venipuncture.
Jonne Terstappen +7 more
openaire +3 more sources
Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays
Bio-Protocol, 2015 Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process.
Jidnyasa Ingale, Richard Wyatt
doaj +1 more source
Cell Reports, 2020 Summary: Rational immunogen design aims to focus antibody responses to vulnerable sites on primary antigens. Given the size of these antigens, there is, however, potential for eliciting unwanted, off-target responses. Here, we use our electron microscopy
Bartek Nogal +10 more
doaj +1 more source
Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population. [PDF]
PLoS ONE, 2013 Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis ...
Xiaoqing Liu +8 more
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Selection of neutralizing antibody escape mutants with type A influenza virus HA-specific polyclonal antisera: possible significance for antigenic drift [PDF]
, 1997 Ten antisera were produced in rabbits by two or three intravenous injections of inactivated whole influenza type A virions. All contained haemagglutination-inhibition (HI) antibody directed predominantly to an epitope in antigenic site B and, in addition,
Cleveland, S. Matthew +2 more
core +2 more sources
A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation
Nature Communications, 2020 Virus neutralization remains the gold standard for determining antibody efficacy. Therefore, a high-throughput assay to measure SARS-CoV-2 neutralizing antibodies is urgently needed for COVID-19 serodiagnosis, convalescent plasma therapy, and vaccine ...
Antonio E. Muruato +6 more
semanticscholar +2 more sources
Neutralizing antibody fails to impact the course of Ebola virus infection in monkeys. [PDF]
PLoS Pathogens, 2007 Prophylaxis with high doses of neutralizing antibody typically offers protection against challenge with viruses producing acute infections. In this study, we have investigated the ability of the neutralizing human monoclonal antibody, KZ52, to protect ...
Wendelien B Oswald +7 more
doaj +1 more source
Protection and mechanism of action of a novel human respiratory syncytial virus vaccine candidate based on the extracellular domain of small hydrophobic protein [PDF]
, 2014 Infections with human respiratory syncytial virus (HRSV) occur globally in all age groups and can have devastating consequences in young infants. We demonstrate that a vaccine based on the extracellular domain (SHe) of the small hydrophobic (SH) protein ...
Bert Schepens +20 more
core +3 more sources