Results 61 to 70 of about 70,788 (268)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Large Eddy Simulation of Shallow Cumulus Clouds in the Southern Great Plains With an Interactive Land Surface Model

Journal of Advances in Modeling Earth Systems
Shallow cumulus clouds are ubiquitous over continental land masses in summertime. They impart complex patterns of solar heating on the surface below. These patterns are dominated by cloud shadows, which drive spatial variability in the surface latent and
Jake J. Gristey, Graham Feingold, Wayne M. Angevine, Yao‐Sheng Chen   +3 more
doaj   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Influence of the details of topography onweather forecast – evaluation of HARMONIEexperiments in the Sochi Olympics domainover the Caucasian mountains

Frontiers in Earth Science, 2016
New fine-resolution surface elevation data was implemented intoHARMONIE-AROME-SURFEX Numerical Weather Prediction (NWP) system. Thegrid-scale mean orography, used as a basis of the model'sterrain-following vertical coordinate, as well as variables ...
Laura eRontu, Clemens eWastl, Sami eNiemela   +2 more
doaj   +1 more source

A new sensitive eddy flux instrumentation [PDF]

Tellus A: Dynamic Meteorology and Oceanography, 1967
An instrument for direct measurement of the turbulent water vapour flux has been constructed and used in a field program in southern Sweden. The instrument consists of wet and dry bulb platinum resistance elements of Australian design and of a new fast response wind instrument. The instantaneous wind vector is measured by the aid of strain gauges fixed
openaire   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Research on B2O3–NaF–SiO2 fluxes for improved fusion defects and mechanical properties of A7N01 alloy with unequal thickness

Journal of Materials Research and Technology
In the aerospace industry, the additive manufacturing of aluminum alloys has encountered significant challenges owing to fusion defects. In this work, the B2O3–NaF–SiO2 fluxes are employed for the first time to improve the fusion defects and mechanical ...
Ning Cui, Yaojie Chao, Yu Tang, Qian Luo, Desheng Li, Sitong Zang, Hai Lin   +6 more
doaj   +1 more source

The Two-layer Surface Energy Balance Parameterization Scheme (TSEBPS) for estimation of land surface heat fluxes [PDF]

Hydrology and Earth System Sciences, 2010
A Two-layer Surface Energy Balance Parameterization Scheme (TSEBPS) is proposed for the estimation of surface heat fluxes using Thermal Infrared (TIR) data over sparsely vegetated surfaces.
X. Xin, Q. Liu
doaj  

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source