Results 151 to 160 of about 3,444,607 (339)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

The Impact of a Multichannel Environment on Television News Viewing: A Longitudinal Study of News Audience Polarization in South Korea

International Journal of Communication, 2012
The abundant choices offered by digital media have raised concerns that news consumption might become polarized into people to either seek out or avoid the news.
Su Jung Kim, James G. Webster
doaj  

Pratiques médiatiques et segmentation des publics de l’information politique en France

Communication
This article analyzes political news consumption during the 2022 French presidential election campaign. Based on a national survey (n = 1,358), it aims to identify how political news consumption and media choices vary according to individuals’ social ...
Franck Bousquet, Julien Figeac, Marie Neihouser   +2 more
doaj   +1 more source

Intertemporal Asset Pricing Without Consumption Data [PDF]

This paper proposes a new way to generalize the insights of static asset pricing theory to a multi-period setting. The paper uses a loglinear approximation to the budget constraint to substitute out consumption from a standard intertemporal asset pricing
John Y. Campbell
core  

News consumption and green habits on the use of circular packaging in online shopping in Taiwan: An extension of the theory of planned behavior. [PDF]

Front Psychol, 2022
Lee YC.
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Beliefs in Conspiracy Theories and Online News Consumption during the onset of the COVID-19 pandemic

Journal of Quantitative Description: Digital Media
Using an original survey covering 17 countries, this paper documents the prevalence of beliefs in conspiracy theories related to the COVID-19 pandemic and characterizes the informational, demographic, and trust profiles of individuals who believe them ...
Soyeon Jin, Jan Zilinsky, Franziska Pradel, Yannis Theocharis   +3 more
doaj   +1 more source

Vintage Capital and Expectations Driven Business Cycles [PDF]

This paper demonstrates that increased optimism about future productivity can generate an immediate economic expansion in a neoclassical model with vintage capital and variable capacity utilization.
Floden, Martin
core