Results 241 to 250 of about 149,500 (265)

Next-generation sequencing as source of SNP and structural variants information to improve breeding programs in Populus nigra

, 2010
Stefania Giacomello, Giusi Zaina, Fabio Marroni, Sara Pinosio, Cristian Del Fabbro, Simone Scalabrin, Federica Cattonaro, Frédérique Bitton, Marie‐Christine Le Paslier, Dominique Brunel, Stéphane Schlub, Catherine Bastien, Véronique Jorge, Jennifer Dewoody, Maurizio Sabatti, Wout Boerjan, Gail Taylor, Patricia Faivre‐Rampant, Michele Morgante   +18 more
openalex   +1 more source

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

FEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang   +13 more
wiley   +1 more source

Comparative single‐cell transcriptomic profiling of patient‐derived renal carcinoma cells in cellular and animal models of kidney cancer

FEBS Open Bio, EarlyView.
We generated and characterized clear cell renal cell carcinoma models using the patient‐derived RCC243 cell line—including cell culture, orthotopic, and metastatic tumors—via single‐cell RNA‐sequencing for comparisons between models and patient tumor datasets.
Richard Huang, Lynn Kee, Alexander Gont, Jalna Meens, Fraser G. Ferens, Meredith S. Irwin, Laurie Ailles, Scott A. Yuzwa, Claire M. Robinson, Michael Ohh   +9 more
wiley   +1 more source

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Next-Generation Sequencing

2016
Endometrial cancers are the most frequently diagnosed gynecological malignancy and were expected to be the seventh leading cause of cancer death among American women in 2015. The majority of endometrial cancers are of serous or endometrioid histology. Most human tumors, including endometrial tumors, are driven by the acquisition of pathogenic mutations
Daphne W. Bell, Matthieu Le Gallo, Fred Lozy   +2 more
openaire   +4 more sources

Next-Generation Sequencing

Neuromuscular Disorders, 2019
Next-generation sequencing (NGS) is a powerful technology that allows for high-throughput multiplex sequencing. This chapter reviews the key steps in NGS wet lab and dry lab processing. The library preparation process prepares the substrates to be sequenced through nucleic acid isolation, fragmentation, isolation, end repair, adapter ligation, size ...
B. Udd, Johanna Palmio, M. Jokela, Tiina Suominen, Sini Penttilä, Sara Lehtinen   +5 more
openaire   +4 more sources

Next-generation sequencing in the clinic

Nature Biotechnology, 2013
Pools of cell lines carrying a variety of known mutations are used to validate the performance of a cancer diagnostic test based on next-generation sequencing.
Jason Y. Park, Larry J. Kricka, FORTINA, PAOLO   +2 more
openaire   +4 more sources

Next Generation Sequencing: Transcriptomics

2021
Fungal transcriptomics is a rising field which has gained attention in the last two decades. The recent democratization of high throughput next-generation sequencing (NGS) techniques for large-scale transcriptomic analysis provided the opportunity to explore transcriptomes at an unprecedented level of resolution.
Sillo, Fabiano
openaire   +1 more source

Next-Generation Sequencing in Cancer

Journal of Maxillofacial and Oral Surgery, 2020
In this article, we provide a gestalt idea about NGS technologies and their applications in cancer research and molecular diagnosis.Next-generation sequencing (NGS) advancements like DNA sequencing and RNA sequencing allow uncovering of genomic, transcriptomic, and epigenomic scenes of individual malignant growths.
S. Vinod Nair, null Madhulaxmi, Gigi Thomas, Ravindran Ankathil   +3 more
openaire   +3 more sources

The chemistry of next-generation sequencing

Nature Biotechnology, 2023
The first large genome fully sequenced by next-generation sequencing (NGS) was that of a bacteriophage using sequencing by synthesis (SBS) as a paradigm. SBS in NGS is underpinned by 'reversible-terminator chemistry'. To grow from proof of concept to being both affordable and practical, SBS needed to overcome a series of challenges, each of which ...
Raphaël Rodriguez, Yamuna Krishnan
openaire   +2 more sources

Next-Generation Sequencing

2017
This chapter summarizes the frequency and role of point mutations and small insertions or deletions (indels) in primary prostate cancer (PCa). Compared to other malignancies, PCa is rather characterized by chromosomal aberrations than by gene mutations.
Sven Perner, Anne Offermann
openaire   +2 more sources