Results 31 to 40 of about 382,279 (282)

Pathogen Genomic Surveillance and Bioinformatics Training and Capacity Development Across Africa

International Journal of Infectious Diseases
Introduction: The recent emergence and re-emergence of infectious diseases across Africa have emphasised the critical need for vigorous pathogen genomic surveillance systems on the continent.
Ms Siddiqah George, Ms Kirsty Lee Garson, Mr Tony Yiquan Li, Mr Perceval Maturure, Professor Nicola Mulder   +4 more
doaj   +1 more source

ddSeeker: a tool for processing Bio-Rad ddSEQ single cell RNA-seq data

BMC Genomics, 2018
Background New single-cell isolation technologies are facilitating studies on the transcriptomics of individual cells. Bio-Rad ddSEQ is a droplet-based microfluidic system that, when coupled with downstream Illumina library preparation and sequencing ...
Dario Romagnoli, Giulia Boccalini, Martina Bonechi, Chiara Biagioni, Paola Fassan, Roberto Bertorelli, Veronica De Sanctis, Angelo Di Leo, Ilenia Migliaccio, Luca Malorni, Matteo Benelli   +10 more
doaj   +1 more source

Standardization of sequencing coverage depth in NGS: Recommendation for detection of clonal and subclonal mutations in cancer diagnostics [PDF]

, 2019
The insufficient standardization of diagnostic next-generation sequencing (NGS) still limits its implementation in clinical practice, with the correct detection of mutations at low variant allele frequencies (VAF) facing particular challenges. We address
Dýšková, Tereza, Kriegová, Eva, Novosad, Tomáš, Papajík, Tomáš, Petráčková, Anna, Schneiderová, Petra, Sedlaříková, Lenka, Vašinek, Michal   +7 more
core   +1 more source

Seq2scFv: a toolkit for the comprehensive analysis of display libraries from long-read sequencing platforms

mAbs
Antibodies have emerged as the leading class of biotherapeutics, yet traditional screening methods face significant time and resource challenges in identifying lead candidates.
Marianne Bachmann Salvy, Luca Santuari, Emanuel Schmid-Siegert, Nikolaos Lykoskoufis, Ioannis Xenarios, Bulak Arpat   +5 more
doaj   +1 more source

Examination of two major approximations used in the scalar airborne gravimetric system — a case study based on the LCR system

Journal of Geodetic Science, 2013
Airborne gravimetry has been proved to be the primary technique to efficiently obtain middle to short wavelength signals of the Earth’s gravity field in regional geodetic applications. In particular, the LCR (LaCoste & Romberg) based scalar system (i.e.,
Li X.
doaj   +1 more source

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Adey, Andrea Gehring, Anna Benet-Pagès, Bainbridge, Bentley, Carsten Bergmann, Clark, Clement, Ding, Dohm, Gerlinger, Gregory, Greif, Greif, Hanno Jörn Bolz, Hanns-Georg Klein, Harismendy, Ina Vogl, Jiang, Johansson, Kaimo Hirv, Kalari, Klaus H. Metzeler, Koboldt, Li, Lister, Loman, Mamanova, Manfred Stuhrmann, Mardis, Marius Kuhn, Mertes, Meyerson, Minoche, Nakamura, Philipp A. Greif, Robinson, Rothberg, Saskia Biskup, Sebastian H. Eck, Shendure, Stefan Kotschote, Stratton   +42 more
core   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

AI-accelerated therapeutic antibody development: practical insights

Frontiers in Drug Discovery
Antibodies represent the largest class of biotherapeutics thanks to their high target specificity, binding affinity and versatility. Recent breakthroughs in Artificial Intelligence (AI) have enabled information-rich in silico representations of ...
Luca Santuari, Marianne Bachmann Salvy, Ioannis Xenarios, Ioannis Xenarios, Bulak Arpat   +4 more
doaj   +1 more source

Atmospheric tomography with separate minimum variance laser and natural guide star mode control [PDF]

, 2009
This paper introduces a novel, computationally efficient, and practical atmospheric tomography wavefront control architecture with separate minimum variance laser and natural guide star mode estimation.
Ellerbroek, B. L., Gilles, L.
core   +1 more source

A new targeted CFTR mutation panel based on next-generation sequencing technology [PDF]

, 2017
Searching for mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR) is a key step in the diagnosis of and neonatal and carrier screening for cystic fibrosis (CF), and it has implications for prognosis and personalized therapy ...
Alberti, Luisella, Biffignandi, Alice, Bruno, Sabina Maria, Colombo, Carla, Corbetta, Carlo, Costantino, Lucy, Giannone, Valentina, Lucarelli, Marco, Porcaro, Luigi, Seia, Manuela, Torresani, Erminio   +10 more
core   +2 more sources