Results 81 to 90 of about 169,956 (263)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Complete plastome sequences of 14 African yam species (Dioscorea spp.)

Mitochondrial DNA. Part B. Resources, 2019
Complete plastomes of 14 African yam species were reconstructed from whole genome sequencing. These plastomes sizes varied from 151,908 to 155,155 bp. We predicted 130 genes, including 18 duplicated genes located in the two inverted regions. Phylogenetic
Jacqueline Magwé-Tindo, Jan J. Wieringa, Bonaventure Sonké, Louis Zapfack, Yves Vigouroux, Thomas L. P. Couvreur, Nora Scarcelli   +6 more
doaj   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Detectarea și analiza mutațiilor somatice în unele probe de adenocarcinom pulmonar prin tehnologia de secvențiere ION TORRENT.

Buletinul Academiei de Ştiinţe a Moldovei: Ştiinţe Medicale, 2019
Cancerul pulmonar este cea mai comună cauză a mortalității prin cancer și cel mai comun cancer din lume cu peste 1,5 milioane de decese anual. Explorarea aspectelor genomice, studiul profilului molecular și selectarea terapiilor țintite pentru aceste ...
Valentina Stratan, Victor Sîtnic, Valeri Ţuţuianu, Valeriu Bîlba, Sergiu Brenişter, Vladimir Şutkin, Ana Lozovanu, Viorel Munteanu, Cristina Popa   +8 more
doaj  

Missense mutations of NCPAG gene affect calving ease in Piedmontese cattle: preliminary evidences

Italian Journal of Animal Science, 2018
A previous genome scan on 323 Piedmontese individuals identified a cluster of 13 SNPs significantly associated with direct calving ease and centred on the three genes LAP3, LCORL and NCAPG in chromosome 6.
Giovanni Chillemi, Silvia Bongiorni, Silvia Gioiosa, Tiziano Flati, Tiziana Castrignanò, Marco Milanesi, Paolo Ajmone Marsan, Alessio Valentini   +7 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

NGS-InVi: Integration and visualization of NGS data

, 2017
Visualization of Next Generation Sequencing (NGS) data is vital to allow researchers to explore and understand the data from experiments or large-scale datasets. A common process involves processing NGS files to create suitable input files (e.g. files in BED format) to be used by visualization software like Circos (http://circos.ca/).
Ricaud, Luciano Francisco Fernández, López, Marcela Dávila   +1 more
openaire   +1 more source