Virus-induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana [PDF]
, 2018 Virus-induced gene silencing (VIGS) is an important forward and reverse genetics method for the study of gene function in many plant species, especially Nicotiana benthamiana.
Chakravarthy, Suma +15 more
core +2 more sources
SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions
Bio-Protocol, 2014 We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants.
Shisong Ma, Savithramma Dinesh-Kumar
doaj +1 more source
Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene [PDF]
E3S Web of Conferences, 2021 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends ...
Zhao Meiwei +4 more
doaj +1 more source
Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
, 2018 Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
core +3 more sources
Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System
Bio-Protocol, 2015 Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N.
Fang Xu, Charles Copeland, Xin Li
doaj +1 more source
The role of the Cucumber mosaic virus 2b protein in viral movement and symptom induction [PDF]
, 2009 The Cucumber mosaic virus (CMV) 2b protein is a counter-defense factor and symptom determinant. Conserved domains in the 2b protein sequence were mutated in the 2b gene of strain Fny-CMV.
Chen H.-Y. +15 more
core +2 more sources
Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco [PDF]
, 2014 The proposed clinical trial in Africa of VRC01, a potent broadly neutralizing antibody (bNAb) capable of neutralizing 91% of known HIV-1 isolates, raises concerns about testing a treatment which will be too expensive to be accessible by the most ...
EMEA +4 more
core +1 more source
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Virology Journal, 2011 Background Some Nicotiana species are widely used as experimental hosts for plant viruses. Nicotiana species differ in ploidy levels, chromosome numbers and have diverse geographical origins.
Mansoor Shahid +2 more
doaj +1 more source
Stromule extension along microtubules coordinated with actin-mediated anchoring guides perinuclear chloroplast movement during innate immunity. [PDF]
, 2018 Dynamic tubular extensions from chloroplasts called stromules have recently been shown to connect with nuclei and function during innate immunity. We demonstrate that stromules extend along microtubules (MTs) and MT organization directly affects stromule
Alqarni, Ali +10 more
core +3 more sources
Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana [PDF]
Plant Biotechnology Journal, 2016 SummaryPlant‐based platforms are extensively used for the expression of recombinant proteins, including monoclonal antibodies. However, to harness the approach effectively and leverage it to its full potential, a better understanding of intracellular processes that affect protein properties is required.
Carolina Gabriela Ocampo +6 more
openaire +4 more sources