Results 21 to 30 of about 24,889 (252)
SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions
Bio-Protocol, 2014 We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants.
Shisong Ma, Savithramma Dinesh-Kumar
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Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene [PDF]
E3S Web of Conferences, 2021 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends ...
Zhao Meiwei +4 more
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Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana [PDF]
Plant Biotechnology Journal, 2016 SummaryPlant‐based platforms are extensively used for the expression of recombinant proteins, including monoclonal antibodies. However, to harness the approach effectively and leverage it to its full potential, a better understanding of intracellular processes that affect protein properties is required.
Carolina Gabriela Ocampo +6 more
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Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System
Bio-Protocol, 2015 Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N.
Fang Xu, Charles Copeland, Xin Li
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Description of the Nicotiana benthamiana−Cercospora nicotianae Pathosystem [PDF]
Phytopathology®, 2018 Nicotiana benthamiana is a valuable model organism in plant biology research. This report describes its extended applicability in the field of molecular plant pathology by introducing a nonbiotrophic fungal pathogen Cercospora nicotianae that can be conveniently used under laboratory conditions, consistently induces a necrotic leaf spot disease on ...
Fodor, József +5 more
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Reactions of
Virology Journal, 2011 Background Some Nicotiana species are widely used as experimental hosts for plant viruses. Nicotiana species differ in ploidy levels, chromosome numbers and have diverse geographical origins.
Mansoor Shahid +2 more
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Physiological Function of NbRanBP1 in Nicotiana benthamiana
Molecules and Cells, 2008 This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily ...
Hui-Kyung, Cho +2 more
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四川大学学报. 自然科学版, 2021 In order to explore the possibility of tobacco rattle virus overexpression and silencing system for oil-related gene research, this study cloned Arabidopsis thaliana WRI1 and FAD2 genes, and constructed TRV overexpression and silencing vectors ...
JIANG Yue +3 more
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Nicotiana benthamiana as a Production Platform for Artemisinin Precursors
PLoS ONE, 2010 Production of pharmaceuticals in plants provides an alternative for chemical synthesis, fermentation or natural sources. Nicotiana benthamiana is deployed at commercial scale for production of therapeutic proteins. Here the potential of this plant is explored for rapid production of precursors of artemisinin, a sesquiterpenoid compound that is used for
van Herpen, T.W.J.M. +5 more
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Reproducibility and flexibility of monoclonal antibody production with Nicotiana benthamiana [PDF]
mAbs, 2022 The ongoing SARS-CoV-2 coronavirus pandemic of 2020-2021 underscores the need for manufacturing platforms that can rapidly produce monoclonal antibody (mAb) therapies. As reported here, a platform based on Nicotiana benthamiana produced mAb therapeutics with high batch-to-batch reproducibility and flexibility, enabling production of 19 different mAbs ...
Kelsi Swope +17 more
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