Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene [PDF]
E3S Web of Conferences, 2021 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends ...
Zhao Meiwei +4 more
doaj +1 more source
In vitro micro propagation of Nicotiana benthamiana via axillary shoots [PDF]
, 2015 Axillary shoots of Nicotiana benthamiana were regenerated from nodal explants in two weeks using MS media supplemented with the cytokinin, kinetin (0.5 mg/L), and the auxin, indole-3-butyric acid (IBA) (0.1 mg/L). Ninety two percent of shoots were 2.1-20
Deo, Pradeep C. +4 more
core +1 more source
Vacuolar targeting of recombinant antibodies in Nicotiana benthamiana [PDF]
Plant Biotechnology Journal, 2016 SummaryPlant‐based platforms are extensively used for the expression of recombinant proteins, including monoclonal antibodies. However, to harness the approach effectively and leverage it to its full potential, a better understanding of intracellular processes that affect protein properties is required.
Carolina Gabriela Ocampo +6 more
openaire +4 more sources
Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System
Bio-Protocol, 2015 Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N.
Fang Xu, Charles Copeland, Xin Li
doaj +1 more source
A cost-effective dual reporter system in <i>Nicotiana benthamiana</i>. [PDF]
Front Plant SciDual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening. Here, we systematically characterized seven commonly used or potential reporters,
Zhang Z +11 more
europepmc +3 more sources
Importin-α-mediated nucleolar localization of potato mop-top virus TRIPLE GENE BLOCK1 (TGB1) protein facilitates virus systemic movement, whereas TGB1 self-interaction is required for cell-to-cell movement in Nicotiana benthamiana [PDF]
, 2015 This work was supported by the Swedish Research Council Formas (to E.I.S.), the Carl Tryggers Foundation (to E.I.S. and N.I.L.), the Swedish Institute (to N.I.L.), and the Rural and Environmental Science and Analytical Services Division strategic ...
Savenkov, Eugene I. +5 more
core +1 more source
Physiological Function of NbRanBP1 in Nicotiana benthamiana
Molecules and Cells, 2008 This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily ...
Hui-Kyung, Cho +2 more
openaire +2 more sources
Reactions of
Virology Journal, 2011 Background Some Nicotiana species are widely used as experimental hosts for plant viruses. Nicotiana species differ in ploidy levels, chromosome numbers and have diverse geographical origins.
Mansoor Shahid +2 more
doaj +1 more source
Acylsugars protect Nicotiana benthamiana against insect herbivory and desiccation. [PDF]
, 2022 peer reviewedKEY MESSAGE: Nicotiana benthamiana acylsugar acyltransferase (ASAT) is required for protection against desiccation and insect herbivory.
Moghe, Gaurav D. +17 more
core +2 more sources
四川大学学报. 自然科学版, 2021 In order to explore the possibility of tobacco rattle virus overexpression and silencing system for oil-related gene research, this study cloned Arabidopsis thaliana WRI1 and FAD2 genes, and constructed TRV overexpression and silencing vectors ...
JIANG Yue +3 more
doaj