Transient Expression of Flavivirus Structural Proteins in Nicotiana benthamiana
Flaviviruses are a threat to public health and can cause major disease outbreaks. Tick-borne encephalitis (TBE) is caused by a flavivirus, and it is one of the most important causes of viral encephalitis in Europe and is on the rise in Sweden. As there is no antiviral treatment available, vaccination remains the best protective measure against TBE ...
Naveed Asghar +10 more
openaire +6 more sources
Citrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues. Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable ...
Silvia Ambrós +6 more
doaj +1 more source
RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin [PDF]
Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways.
Garcia, Maria Laura +2 more
core +2 more sources
Virus-induced gene complementation reveals a transcription factor network in modulation of tomato fruit ripening [PDF]
Plant virus technology, in particular virus-induced gene silencing, is a widely used reverse- and forward-genetics tool in plant functional genomics. However the potential of virus technology to express genes to induce phenotypes or to complement mutants
Fan, Zaifeng +15 more
core +3 more sources
Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT
Background The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant’s genomic and transcriptomic features.
Matteo Schiavinato +4 more
doaj +1 more source
Members of the XB3 family from diverse plant species induce programmed cell death in Nicotiana benthamiana. [PDF]
Programmed cell death has been associated with plant immunity and senescence. The receptor kinase XA21 confers resistance to bacterial blight disease of rice (Oryza sativa) caused by Xanthomonas oryzae pv. oryzae (Xoo). Here we show that the XA21 binding
Xiaoen Huang +4 more
doaj +1 more source
Physiological Function of NbRanBP1 in Nicotiana benthamiana
This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily ...
Hui-Kyung, Cho +2 more
openaire +2 more sources
Antimicrobial peptides (AMPs) are next-generation antibiotics that can be used to combat drugresistant pathogens. Here, the authors report efficient production of bioactive amidated AMPs by transient expression in Nicotiana benthamiana line expressing ...
Shahid Chaudhary +13 more
doaj +1 more source
High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity [PDF]
The plant innate immune system employs plasma membrane-localized receptors that specifically perceive pathogen/microbe-associated molecular patterns (PAMPs/MAMPs).
Aragon +68 more
core +1 more source
Virus Infection Suppresses Nicotiana benthamiana Adaptive Phenotypic Plasticity
Competition and parasitism are two important selective forces that shape life-histories, migration rates and population dynamics. Recently, it has been shown in various pathosystems that parasites can modify intraspecific competition, thus generating an indirect cost of parasitism.
Stéphanie Bedhomme, Santiago F Elena
openaire +7 more sources

