Results 51 to 60 of about 44,886 (288)

UBP12 and UBP13 negatively regulate the activity of the ubiquitin-dependent peptidases DA1, DAR1 and DAR2 [PDF]

open access: yes, 2020
Protein ubiquitination is a very diverse post-translational modification leading to protein degradation or delocalization, or altering protein activity. In Arabidopsis thaliana, two E3 ligases, BIG BROTHER (BB) and DA2, activate the latent peptidases DA1,
Chen, Ying   +10 more
core   +1 more source

Influence of retinoblastoma-related gene silencing on the initiation of DNA replication by African cassava mosaic virus Rep in cells of mature leaves in Nicotiana benthamiana plants [PDF]

open access: yes, 2011
Background Geminiviruses mainly infect terminally differentiated tissues and cells in plants. They need to reprogramme host cellular machinery for DNA replication.
Bruce, Gareth   +4 more
core   +2 more sources

Pathogen manipulation of chloroplast function triggers a light-dependent immune recognition [PDF]

open access: yes, 2020
In plants and animals, nucleotide-binding leucine-rich repeat (NLR) proteins are intracellular immune sensors that recognize and eliminate a wide range of invading pathogens.
Bozkurt, Tolga O.   +13 more
core   +1 more source

Stable transformation of Nicotiana benthamiana v1 [PDF]

open access: yes, 2018
Detailed protocol with pictures for the transformation of Nicotiana benthamiana. The same protocol will also work for transformation of Nicotiana tabaccum, but regeneration of transformed plants will take more time.
Jana Ordon   +3 more
openaire   +1 more source

Integrated Single‐Cell and Spatial Transcriptomics Reveal Cell‐Type‐Specific Immune Regulatory Networks in Maize Responding to Southern Corn Rust

open access: yesAdvanced Science, EarlyView.
By integrating single‐nuclei and spatial transcriptomics, this study presents a stereoscopic landscape of maize leaf to Puccinia polysora infection. Epidermal and mesophyll cells initiate primary defenses via RLPs/RLKs and jasmonic acid signaling. Cell‐cell communication analyses further reveal the underlying the dynamics of the underlying immune ...
Qiongqiong Wang   +16 more
wiley   +1 more source

Discovery of a Plant Pictet–Spenglerase With R‐Stereoselectivity

open access: yesAngewandte Chemie, EarlyView.
Strictosidine synthases (STRs) are plant Pictet–Spenglerases catalyzing the coupling of a secoiridoid with tryptamine. All previously characterized strictosidine synthases are strictly S‐selective. Here, we report the discovery of Epi‐STR, an R‐selective STR ortholog from the medicinal plant Pogonopus speciosus.
Clara Morweiser   +3 more
wiley   +2 more sources

Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana

open access: yesBio-Protocol, 2018
Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens.
Teruhiko Kuroyanagi   +2 more
doaj   +1 more source

Overexpression of Nictaba-like lectin genes from glycine max confers tolerance towards Pseudomonas syringae infection, aphid infestation and salt stress in transgenic Arabidopsis plants [PDF]

open access: yes, 2016
Plants have evolved a sophisticated immune system that allows them to recognize invading pathogens by specialized receptors. Carbohydrate-binding proteins or lectins are part of this immune system and especially the lectins that reside in the ...
Smagghe, Guy   +2 more
core   +3 more sources

Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana [PDF]

open access: yesPlant Methods, 2018
Agroinfiltration is a simple and effective method of delivering transgenes into plant cells for the rapid production of recombinant proteins and has become the preferred transient expression platform to manufacture biologics in plants. Despite its popularity, few studies have sought to improve the efficiency of agroinfiltration to further increase ...
Karlah Norkunas   +3 more
openaire   +4 more sources

Mass Spectrometry Imaging‐Assisted Discovery of Gallotannin Biosynthetic Genes in the Root of Paeonia suffruticosa

open access: yesAdvanced Science, EarlyView.
An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu   +4 more
wiley   +1 more source

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