Results 51 to 60 of about 24,889 (252)
Molecular Plant-Microbe Interactions, 2009 An overexpression screen of Nicotiana benthamiana cDNAs identified a gene for the LCB2 subunit of serine palmitoyltransferase (SPT) as a potent inducer of hypersensitive response-like cell death.
Yoshihiro Takahashi +6 more
doaj +1 more source
Agroinfiltration and PVX agroinfection in potato and Nicotiana benthamiana.
Journal of visualized experiments : JoVE, 2014 Agroinfiltration and PVX agroinfection are two efficient transient expression assays for functional analysis of candidate genes in plants. The most commonly used agent for agroinfiltration is Agrobacterium tumefaciens, a pathogen of many dicot plant species. This implies that agroinfiltration can be applied to many plant species.
Du, J. +2 more
openaire +3 more sources
Reconstitution of the Costunolide Biosynthetic Pathway in Yeast and Nicotiana benthamiana
PLoS ONE, 2011 The sesquiterpene costunolide has a broad range of biological activities and is the parent compound for many other biologically active sesquiterpenes such as parthenolide. Two enzymes of the pathway leading to costunolide have been previously characterized: germacrene A synthase (GAS) and germacrene A oxidase (GAO), which together catalyse the ...
Liu, Q. +9 more
openaire +6 more sources
Advanced Science, EarlyView.Floral meristem (FM) activity is crucial for the initiation of floral organs. In this study, SE represses IPT7 expression and cytokinin signaling via both the miR171c‐5p–KNAT2 and miR164c–CUC1/2–KNAT2 regulatory modules, thereby repressing FM activity. In contrast, STM induces both KNAT2 and IPT7 expression.
Wen Yang +11 more
wiley +1 more source
Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum
Plant Protection Science, 2002 Resistance to Potato mop-top virus (PMTV) was studied in Nicotiana benthamiana and potato (Solanum tuberosum cv.Saturna) transformed with the coat protein (CP) gene of PMTV. In N.
A. Germundsson +4 more
doaj +1 more source
Transposase‐Assisted Donor Tethering Boosts Large‐Fragment HDR in Plants
Advanced Science, EarlyView.A transposase‐assisted donor tethering strategy is developed to enhance homology‐directed repair in plants. By recruiting donor DNA to double‐strand breaks and synergizing with repair pathway reprogramming and transcription‐coupled donor design, this system markedly improves large‐fragment targeted insertion efficiency, providing a robust platform for ...
Sha Wei +8 more
wiley +1 more source
BMC Genomics, 2019 Background Nicotiana benthamiana is an important model organism of the Solanaceae (Nightshade) family. Several draft assemblies of the N. benthamiana genome have been generated, but many of the gene-models in these draft assemblies appear incorrect ...
Jiorgos Kourelis +5 more
doaj +1 more source
PlantGFM: A Genomic Foundation Model for Discovery and Creation of Plant Genes
Advanced Science, EarlyView.A plant genomic foundation model pre‐trained on 12 species enables both accurate gene prediction and de novo gene design. Through AI‐human knowledge screening, seven designed sequences showed transcriptional activity in plants, with two expressing stable proteins—demonstrating the first DNA‐RNA‐protein expression of LLM‐generated genes in plants and ...
Changhao Li +10 more
wiley +1 more source
Фактори експериментальної еволюції організмів, 2015 Aims. The use of plants as a recombinant peptides expression system is a valuable alternative for the production of collagen for various medical and scientific purposes.
Н. Л. Щербак +2 more
doaj
Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta
Molecular Plant-Microbe Interactions, 2000 The ability of the Pseudomonas syringae pv. phaseolicola harpin (HrpZPsph) to elicit hypersensitive response was investigated in three Nicotiana genotypes. The hrpZPsph gene was placed under chemical regulation (tetracycline induction) in TetR+ Nicotiana
Anastasia P. Tampakaki +1 more
doaj +1 more source