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The bacterial nitrate reductases: EPR studies on nitrate reductase A from Micrococcus denitrificans

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
EPR studies on nitrate reductase indicate at 80 °K a signal at g=1.985 and g=2.045 attributable to Mo(V). At 15 °K a signal is observed at g=2.016 due to Fe(III) in an unknown symmetry. The reaction of nitrate reductase with nitrate, nitrite or azide results in changes of the Mo(V) signal.
Daniel V. DerVartanian, P. Forget
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Evolution of the soluble nitrate reductase: defining the monomeric periplasmic nitrate reductase subgroup

Biochemical Society Transactions, 2006
Bacterial nitrate reductases can be classified into at least three groups according to their localization and function, namely membrane-bound (NAR) or periplasmic (NAP) respiratory and cytoplasmic assimilatory (NAS) enzymes. Monomeric NASs are the simplest of the soluble nitrate reductases, although heterodimeric NASs exist, and a common structural ...
Jepson, B. J. N.   +5 more
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Molecular evolution of nitrate reductase genes

Journal of Molecular Evolution, 1996
To understand the evolutionary mechanisms and relationships of nitrate reductases (NRs), the nucleotide sequences encoding 19 nitrate reductase (NR) genes from 16 species of fungi, algae, and higher plants were analyzed. The NR genes examined show substantial sequence similarity, particularly within functional domains, and large variations in GC ...
Jizhong Zhou, Andris Kleinhofs
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Stable Transformation of Chlorella : Rescue of Nitrate Reductase-Deficient Mutants with the Nitrate Reductase Gene

Current Microbiology, 1997
Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce the nitrate reductase (NR) gene from
Andrew C. Cannons   +2 more
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Degradation of explosives by nitrate ester reductases

Biochemical Society Symposia, 2001
Explosive-contaminated land poses a hazard both to the environment and to human health. Microbial enzymes, either in their native or heterologous hosts, are a powerful and low-cost tool for eliminating this environmental hazard. As many explosives have only been present in the environment for 10 years, and with similar molecules not known in Nature ...
Williams, Richard E.   +4 more
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In vitro reconstitution of NADH: nitrate reductase in nitrate reductase-deficient mutants of barley

Molecular and General Genetics MGG, 1984
In vitro complementation of the nitrate reductase-deficient barley mutant nar2a extracts with molybdenum cofactor from commercial xanthine oxidase resulted in reactivation of NADH: nitrate reductase activity. Maximum reactivation was achieved with 7.5 μg/ml xanthine oxidase (final concentration), 10 mM glutathione (final concentration) and incubation ...
Andris Kleinhofs   +3 more
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Formation of active heterologous nitrate reductases between nitrate reductases A and Z of Escherichia coli

Molecular Microbiology, 1992
SummaryTwo nitrate reductases, NRA and NRZ, are present in Escherichia coli. These isoenzymes have the same αβγ, subunits composition and have similar size and genetic organization. Corresponding subunits of the complexes share at least 75% identity.
Blasco, Francis   +5 more
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nitrate reductase and nitric oxide

2010
International ...
Kaiser, Werner   +2 more
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The Nitrate Reductase System

1984
It has been proposed by Klemm and Ninnemann [29] that nitrate reductase (NR) may act as a photoreceptor in blue light-stimulated conidiation of Neurospora crassa (carotenoid-less mutant al-2 bd). Nitrate reduction and conidiation appear to be correlated in some specific ways: Hochberg and Sargent [19] and Weiss and Turian [61] reported that conidiation
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Functional domains of assimilatory nitrate reductases and nitrite reductases

Trends in Biochemical Sciences, 1990
Biochemical investigation of nitrate assimilation enzymes spans the past four decades. With the molecular cloning of genes for nitrate reductases and nitrite reductases, exciting new prospects are developing for the study of these enzymes. As large, complex enzymes with multiple redox centers, these two types of reductases should help us gain ...
Wilbur H. Campbell, James R. Kinghorn
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