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Stable Transformation of Chlorella : Rescue of Nitrate Reductase-Deficient Mutants with the Nitrate Reductase Gene

Current Microbiology, 1997
Unicellular green algae, like Chlorella, offer a potentially useful system for the expression of heterologous proteins. However, the development of Chlorella as a bioreactor has been delayed owing to the lack of a stable transformation technique. Here we report on the use of micro-projectile bombardment to introduce the nitrate reductase (NR) gene from
Andrew C. Cannons   +2 more
openaire   +3 more sources

Degradation of explosives by nitrate ester reductases

Biochemical Society Symposia, 2001
Explosive-contaminated land poses a hazard both to the environment and to human health. Microbial enzymes, either in their native or heterologous hosts, are a powerful and low-cost tool for eliminating this environmental hazard. As many explosives have only been present in the environment for 10 years, and with similar molecules not known in Nature ...
Williams, Richard E.   +4 more
openaire   +4 more sources

A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater.

Analytical Chemistry, 2001
We report a new method for measurement of the isotopic composition of nitrate (NO3-) at the natural-abundance level in both seawater and freshwater.
D. Sigman   +5 more
semanticscholar   +1 more source

In vitro reconstitution of NADH: nitrate reductase in nitrate reductase-deficient mutants of barley

Molecular and General Genetics MGG, 1984
In vitro complementation of the nitrate reductase-deficient barley mutant nar2a extracts with molybdenum cofactor from commercial xanthine oxidase resulted in reactivation of NADH: nitrate reductase activity. Maximum reactivation was achieved with 7.5 μg/ml xanthine oxidase (final concentration), 10 mM glutathione (final concentration) and incubation ...
Andris Kleinhofs   +3 more
openaire   +2 more sources

Formation of active heterologous nitrate reductases between nitrate reductases A and Z of Escherichia coli

Molecular Microbiology, 1992
SummaryTwo nitrate reductases, NRA and NRZ, are present in Escherichia coli. These isoenzymes have the same αβγ, subunits composition and have similar size and genetic organization. Corresponding subunits of the complexes share at least 75% identity.
Blasco, Francis   +5 more
openaire   +3 more sources

The Nitrate Reductase System

1984
It has been proposed by Klemm and Ninnemann [29] that nitrate reductase (NR) may act as a photoreceptor in blue light-stimulated conidiation of Neurospora crassa (carotenoid-less mutant al-2 bd). Nitrate reduction and conidiation appear to be correlated in some specific ways: Hochberg and Sargent [19] and Weiss and Turian [61] reported that conidiation
openaire   +2 more sources

Functional domains of assimilatory nitrate reductases and nitrite reductases

Trends in Biochemical Sciences, 1990
Biochemical investigation of nitrate assimilation enzymes spans the past four decades. With the molecular cloning of genes for nitrate reductases and nitrite reductases, exciting new prospects are developing for the study of these enzymes. As large, complex enzymes with multiple redox centers, these two types of reductases should help us gain ...
Wilbur H. Campbell, James R. Kinghorn
openaire   +3 more sources

Nitrate Reductase

Research Methods of Environmental Physiology in Aquatic Sciences, 2020
D. Zou
semanticscholar   +1 more source

Nitrate reductase (NADH) [PDF]

open access: possible, 1994
Dietmar Schomburg   +2 more
openaire   +2 more sources

Nitrate Reductase Regulates Plant Nitric Oxide Homeostasis.

Trends in Plant Science, 2017
Alejandro Chamizo-Ampudia   +4 more
semanticscholar   +1 more source

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