Results 21 to 30 of about 136,576 (266)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Denitrifier Still Has the Important Role in Nitrate Reduction to N2 Although It is Not the Predominant Population in the Estuarine Bacterial Community of Nitrate Reducing Bacteria

Hayati Journal of Biosciences
Denitrification and nitrate-ammonification are the responsible processes for nitrate removal in the estuaries. Temperature, nitrate and organic carbon availability are key factors controlling a rate of the nitrate reduction processes.
Iman Rusmana, David B. Nedwell
doaj   +1 more source

Rapid Nitrate Reduction Assay with Intact Microbial Cells or Spores

Bio-Protocol, 2014
Many microorganisms have the capacity to use nitrate as a respiratory electron acceptor. Reduction of nitrate is catalyzed by a multi-subunit nitrate reductase that is often located associated with the cytoplasmic membrane and has its active site ...
Marco Fischer, Dörte Falke, Gary Sawers   +2 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Alternative Oxidase Alleviates Mitochondrial Oxidative Stress during Limited Nitrate Reduction in Arabidopsis thaliana

Biomolecules
The conversion of nitrate to ammonium, i.e., nitrate reduction, is a major consumer of reductants in plants. Previous studies have reported that the mitochondrial alternative oxidase (AOX) is upregulated under limited nitrate reduction conditions ...
Daisuke Otomaru, Natsumi Ooi, Kota Monden, Takamasa Suzuki, Ko Noguchi, Tsuyoshi Nakagawa, Takushi Hachiya   +6 more
doaj   +1 more source

A Review of Environment Effects on Nitrate Accumulation in Leafy Vegetables Grown in Controlled Environments

Foods, 2020
Excessive accumulation of nitrates in vegetables is a common issue that poses a potential threat to human health. The absorption, translocation, and assimilation of nitrates in vegetables are tightly regulated by the interaction of internal cues ...
Zhonghua Bian, Yu Wang, Xiaoyan Zhang, Tao Li, Steven Grundy, Qichang Yang, Ruifeng Cheng   +6 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Interaction between nitrate and trichloroethene bioreduction in mixed anaerobic cultures

Frontiers in Microbiology
Bioremediation of trichloroethene (TCE)-contaminated sites often leads to groundwater acidification, while nitrate-polluted sites tend to generate alkalization.
Dong-Mei Yang, Fen-Li Min, Ying Li, Jia-Lu Ling, Hui-Xian Zhong, Yu-Chun Xia, Ying Feng, Li-Ya Zhao, Zhao-Hua Li, Li-Lian Wen   +9 more
doaj   +1 more source

Fabrication of Nanostructured Copper Thin Films at Disposable Pencil Graphite Electrode and its Application to Elecrocatalytic Reduction of Nitrate

International Journal of Electrochemical Science, 2011
In this work, a galvanostatic double-pulse technique was presented for fabrication of nanostructured copper thin films. Multi-layer structure was obtained for copper deposits.
Mir Reza Majidi, K. Asadpour-Zeynali, B. Hafezi   +2 more
doaj   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source