Results 31 to 40 of about 2,342 (296)

Direct Determination of Nitrate Using Nitrate Reductase in a Flow System.

Journal of Health Science, 2000
Nitrate was determined using nitrate reductase (NR) in a flow system. A merging zone method was applied in the system in which a zone of NR and that of nitrate in separate streams were merged to react. The NADPH decreased by the enzymatic reaction was detected at 340 nm. The length of the reaction coil used for the enzymatic reaction was 250 cm. Of the
openaire   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Assessing the Influence of Summer Organic Fertilization Combined with Nitrogen Inhibitor on a Short Rotation Woody Crop in Mediterranean Environment

International Journal of Forestry Research, 2014
The European Union Directive 91/676/EEC, known as Nitrates Directive, has dictated basic agronomic principles regarding the use of animal manure source as well as livestock and waste waters from small food companies.
Anita Maienza, Giovanni Mughini, Luca Salvati, Anna Benedetti, Maria Teresa Dell'Abate   +4 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Protection de la ressource en eau du bassin de l’Auxerrois et transformation des pratiques agricoles

VertigO, 2021
With nitrate levels exceeding the thresholds defined by the European "nitrates" Directive, and the presence of pesticide residues used by the sector's agricultural activities, the water in the catchment areas of the Auxerrois has been the subject of ...
Simon Calla, Sébastien Dassé, Lou Lécuyer, Juliette Young   +3 more
doaj   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Wetland restoration and nitrate reduction: the example of the periurban wetland of Vitoria-Gasteiz (Basque Country, North Spain) [PDF]

, 2003
Changes in land use and agricultural intensification caused wetlands on the quaternary aquifer of Vitoria-Gasteiz (Basque Country) to disappear some years ago and nitrate concentration in groundwaters increased very quickly.
Martínez-Bilbao, V., J. M. Sánchez-Pérez, M. Martínez-Santos, C. García-Linares, Sánchez-Pérez, J. M., V. Martínez-Bilbao, Martínez-Santos, M., I. Antiguedad, Martínez-Santos, Miren, Sanchez-Pérez, José-Miguel, Antigüedad, Iñaki, Antiguedad, I., C. García-Linares, García-Linares, Cristina, García-Linares, C.   +14 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

FEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

STUDY ON THE PHYSICOCHEMICAL AND CHEMICAL PARAMETERS OF DRINKING AND SURFACE WATERS FROM MINE AREA AT VILLAGE BOV, BALKAN MOUNTAIN, BULGARIA

Studia Universitatis Babes-Bolyai Chemia, 2018
Water quality is influenced by natural local climate, geology and anthropogenic effects. In the present study, surface waters in hydrographic areas situated in a mining region, in the vicinity of an abandoned copper mine, are characterized and their ...
Metodi MLADENOV, Irina KARADJOVA, Galia GENTSCHEVA, Albena PREDOEVA   +3 more
doaj   +1 more source