Results 271 to 280 of about 50,424 (297)
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Structure of cytochrome c nitrite reductase
Nature, 1999The enzyme cytochrome c nitrite reductase catalyses the six-electron reduction of nitrite to ammonia as one of the key steps in the biological nitrogen cycle, where it participates in the anaerobic energy metabolism of dissimilatory nitrate ammonification.
Einsle, Oliver +6 more
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Nitrite Reductases in Denitrification
2007Nitrite reductase (NIR) is a key enzyme in the dissimilatory denitrification chain, catalyzing the reduction of NO2 - to NO. Although a matter of debate for a long time, NO is now accepted product of NO2 --reduction as an obligatory intermediate in most denitrifiers, and it is further reduced to N2O by NO reductase. Purification and characterization of
RINALDO, Serena, CUTRUZZOLA', Francesca
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1971
Publisher Summary This chapter discusses the determination of nitrite reductase. The usual assay involves sodium dithionite as reductant and either ferredoxin or its artificial substitute, methyl viologen, as the electron carrier. Enzymatic activity can be best followed by measuring colorimetrically the rate of disappearance of nitrite.
Antonio Paneque, M. Losada
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Publisher Summary This chapter discusses the determination of nitrite reductase. The usual assay involves sodium dithionite as reductant and either ferredoxin or its artificial substitute, methyl viologen, as the electron carrier. Enzymatic activity can be best followed by measuring colorimetrically the rate of disappearance of nitrite.
Antonio Paneque, M. Losada
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A dissimilatory nitrite reductase in Paracoccus halodenitrificans
Archives of Microbiology, 1984Paracoccus halodenitrificans produced a membrane-associated nitrite reductase. Spectrophotometric analysis showed it to be associated with a cd-cytochrome and located on the inner side of the cytoplasmic membrane. When supplied with nitrite, membrane preparations produced nitrous oxide and nitric oxide in different ratios depending on the electron ...
Michael A. Grant, Lawrence I. Hochstein
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A nitrite reductase from Neurospora crassa
Biochimica et Biophysica Acta, 1960Abstract Nitrite reductase has been purified, over 50-fold from ordinary felts of Neurospora crassa, macroconidial wild type Em 5297a. The enzyme is a DPNH-dependent flavoprotein containing FAD, Fe and Cu and -SH groups. Copper may act by coupling the flavin component of the enzyme to nitrite since an external supply of Cu1+ reduced NO2 non ...
D. J. D. Nicholas +2 more
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Octaheme nitrite reductases: Structure and properties
Biochemistry (Moscow), 2012Octaheme oxidoreductases are widespread among various bacterial taxa involved in the biogeochemical nitrogen cycle. The evolution of octaheme oxidoreductases of the nitrogen cycle from the evolutionarily more ancient pentaheme nitrite reductases was accompanied by changes in function from reduction of nitrogen oxides to their oxidation under changing ...
A.A. Trofimov +3 more
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A spectrophotometric assay for dissimilatory nitrite reductases
Analytical Biochemistry, 1988A spectrophotometric assay for dissimilatory nitrite reductases has been developed utilizing mammalian cytochrome c (equine heart) as reductant and spectrophotometric agent. The copper-containing nitrite reductase from Achromobacter cycloclastes has been shown to have apparent Km's for reduced cytochrome c and nitrite of 86 +/- 5 and 5.63 +/- 0.03 ...
Charles L. Hulse +2 more
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Regulation of nitrite uptake and nitrite reductase expression in Chlamydomonas reinhardtii
Biochimica et Biophysica Acta (BBA) - General Subjects, 1991Expression of nitrite uptake and nitrite reductase activities has been studied in Chlamydomonas reinhardtii under different nutritional conditions. Both activities were expressed at a low level in derepressed cells (with no nitrogen source) and at a high level in induced cells (with nitrate or nitrite).
Jacobo Cárdenas +3 more
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The anoxic plant mitochondrion as a nitrite: NO reductase
Mitochondrion, 2011Under the conditions of oxygen deprivation, accumulating nitrite can be reduced in the mitochondrial electron transport chain forming free radical nitric oxide (NO). By reducing nitrite to NO, plant mitochondria preserve the capacity to oxidize external NADH and NADPH and retain a limited power for ATP synthesis complementing glycolytic ATP production.
Abir U. Igamberdiev +1 more
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Ferredoxin-nitrite reductase from spinach
Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966Summary 1. Nitrite reductase from a crude homogenate of spinach leaves has been purified about 500-fold and freed from NADP-reductase (EC 1.6.99.4) and nitrate reductase (EC 1.6.6.2). The enzyme, which does not seem to be a flavoprotein, catalyzes the reduction of nitrite to ammonia with a variety of enzyme systems as electron donors and requires
F.F. Del Campo +3 more
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