Results 31 to 40 of about 2,207,670 (307)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Rice is an important target to improve crop nitrogen (N) use efficiency (NUE), and the identification and shortlisting of the candidate genes are still in progress.
Narendra Sharma +4 more
doaj +1 more source
Liver organoids: modelling complexity in homeostasis and disease
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
Identification of genotypic variation for nitrogen response in potato (Solanum tuberosum) under low nitrogen input circumstances [PDF]
Nitrogen is an essential nutrient for crop growth. The demand for nitrogen in the potato crop is relatively high. However, in organic farming nitrogen input is rather limited, compared with conventional farming.
Tiemens-Hulscher, M. +2 more
core
The development of dynamically responsive soft porous crystals (SPCs) is crucial for advanced gas separation due to their stimuli-triggered structural transitions.
Yongzheng Wang +7 more
doaj +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Leucaena wood production and use
50 páginas : ilustracionesLa publicación es un manual práctico preparado por Nitrogen Fixing Tree Association y un grupo de autores durante la conferencia sobre leucaena (Singapore, Nov ...
core +1 more source
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante +10 more
wiley +1 more source
Polynitromethyl explosives exhibit outstanding detonation performance, yet their application is significantly hindered by safety concerns. Thus, enhancing the stability of polynitromethyl high-energy density materials (HEDMs) while maintaining high ...
Qi Xue +8 more
doaj +1 more source

